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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
06/02/2017 |
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Data da última atualização: |
30/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SIQUEIRA, L. G. B.; HANSEN, P. J. |
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Afiliação: |
LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; PETER J. HANSEN, University of Florida, Gainesville, Florida, USA. |
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Título: |
Sex differences in response of the bovine embryo to colony-stimulating factor 2. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Reproduction, n. 152, p. 645-654, 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
We tested whether gene expression of the bovine morula is modified by CSF2 in a sex-dependent manner and if sex determines the effect of CSF2 on competence of embryos to become blastocysts. Embryos were produced in vitro using X- or Y-sorted semen and treated at Day 5 of culture with 10 ng/mL bovine CSF2 or control. In experiment 1, morulae were collected at Day 6 and biological replicates (n = 8) were evaluated for transcript abundance of 90 genes by RT-qPCR using the Fluidigm Delta Gene assay. Expression of more than one-third (33 of 90) of genes examined was affected by sex. The effect of CSF2 on gene expression was modified by sex (P < 0.05) for five genes (DDX3Y/DDX3X-like, NANOG, MYF6, POU5F1 and RIPK3) and tended (P < 0.10) to be modified by sex for five other genes (DAPK1, HOXA5, PPP2R3A, PTEN and TNFSF8). In experiment 2, embryos were treated at Day 5 with control or CSF2 and blastocysts were collected at Day 7 for immunolabeling to determine the number of inner cell mass (ICM) and trophectoderm (TE) cells. CSF2 increased the percent of putative zygotes that became blastocysts for females, but did not affect the development of males. There was no effect of CSF2 or interaction of CSF2 with sex on the total number of blastomeres in blastocysts or in the number of inner cell mass or trophectoderm cells. In conclusion, CSF2 exerted divergent responses on gene expression and development of female and male embryos. These results are evidence of sexually dimorphic responses of the preimplantation embryo to this embryokine. MenosWe tested whether gene expression of the bovine morula is modified by CSF2 in a sex-dependent manner and if sex determines the effect of CSF2 on competence of embryos to become blastocysts. Embryos were produced in vitro using X- or Y-sorted semen and treated at Day 5 of culture with 10 ng/mL bovine CSF2 or control. In experiment 1, morulae were collected at Day 6 and biological replicates (n = 8) were evaluated for transcript abundance of 90 genes by RT-qPCR using the Fluidigm Delta Gene assay. Expression of more than one-third (33 of 90) of genes examined was affected by sex. The effect of CSF2 on gene expression was modified by sex (P < 0.05) for five genes (DDX3Y/DDX3X-like, NANOG, MYF6, POU5F1 and RIPK3) and tended (P < 0.10) to be modified by sex for five other genes (DAPK1, HOXA5, PPP2R3A, PTEN and TNFSF8). In experiment 2, embryos were treated at Day 5 with control or CSF2 and blastocysts were collected at Day 7 for immunolabeling to determine the number of inner cell mass (ICM) and trophectoderm (TE) cells. CSF2 increased the percent of putative zygotes that became blastocysts for females, but did not affect the development of males. There was no effect of CSF2 or interaction of CSF2 with sex on the total number of blastomeres in blastocysts or in the number of inner cell mass or trophectoderm cells. In conclusion, CSF2 exerted divergent responses on gene expression and development of female and male embryos. These results a... Mostrar Tudo |
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Palavras-Chave: |
CSF2; Embryokine. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/154808/1/Cnpgl-2016-Reproduction-Sex.pdf
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Marc: |
LEADER 02045naa a2200157 a 4500
001 2062824
005 2023-01-30
008 2016 bl uuuu u00u1 u #d
100 1 $aSIQUEIRA, L. G. B.
245 $aSex differences in response of the bovine embryo to colony-stimulating factor 2.$h[electronic resource]
260 $c2016
520 $aWe tested whether gene expression of the bovine morula is modified by CSF2 in a sex-dependent manner and if sex determines the effect of CSF2 on competence of embryos to become blastocysts. Embryos were produced in vitro using X- or Y-sorted semen and treated at Day 5 of culture with 10 ng/mL bovine CSF2 or control. In experiment 1, morulae were collected at Day 6 and biological replicates (n = 8) were evaluated for transcript abundance of 90 genes by RT-qPCR using the Fluidigm Delta Gene assay. Expression of more than one-third (33 of 90) of genes examined was affected by sex. The effect of CSF2 on gene expression was modified by sex (P < 0.05) for five genes (DDX3Y/DDX3X-like, NANOG, MYF6, POU5F1 and RIPK3) and tended (P < 0.10) to be modified by sex for five other genes (DAPK1, HOXA5, PPP2R3A, PTEN and TNFSF8). In experiment 2, embryos were treated at Day 5 with control or CSF2 and blastocysts were collected at Day 7 for immunolabeling to determine the number of inner cell mass (ICM) and trophectoderm (TE) cells. CSF2 increased the percent of putative zygotes that became blastocysts for females, but did not affect the development of males. There was no effect of CSF2 or interaction of CSF2 with sex on the total number of blastomeres in blastocysts or in the number of inner cell mass or trophectoderm cells. In conclusion, CSF2 exerted divergent responses on gene expression and development of female and male embryos. These results are evidence of sexually dimorphic responses of the preimplantation embryo to this embryokine.
653 $aCSF2
653 $aEmbryokine
700 1 $aHANSEN, P. J.
773 $tReproduction$gn. 152, p. 645-654, 2016.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| 8. |  | MARIN, F. R.; ANGELOCCI, L. R.; RIGHI, E. Z. Preliminary evaluation of sap flow data by stem heat balance method. In: REUNIÓN ARGENTINA Y LATINOAMERICANA DE AGROMETEOROLOGIA, 10., 4., 2004, Mar del Plata, Argentina. [Anais]: Agrometeorologia y Seguridad Alimentaria en América Latina. Mar del Plata, Argentina: AADA, 2004. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Territorial. |
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| 11. | | MARIN, F. R.; RIBEIRO, R. V.; ANGELOCCI, L. R.; OLIVEIRA, R. F. de. Avaliação do método do balanço de calor para medida da transpiração de mudas de laranjeira e cafeeiro. In: CONGRESSO BRASILEIRO DE AGROMETEOROLOGIA, 13, 2003, Santa Maria. Anais... Santa Maria: UNIFRA, SBA, UFSM, 2003. 79-80 folhas avulsas| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Territorial. |
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| 12. | | SAKAI, R. H.; MARIN, F. R.; NASSIF, D. S. P.; ANGELOCCI, L. R.; RIGHI, E. Z. Condutância foliar e transpiração em cafeeiro irrigado de cinco anos de cultivo. In: CONGRESSO BRASILEIRO DE AGROMETEOROLOGIA, 16, 2009, Belo Horizonte. Anais... Viçosa, MG: UFV; Sete Lagoas: Embrapa Milho e Sorgo, 2009. Não paginado. 1 CD-ROM. CBA 2009.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Agricultura Digital. |
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| 13. | | DELGADO-ROJAS, J. S.; ANGELOCCI, L. R.; FOLEGATTI, M. V.; COELHO FILHO, M. A. Desempenho da sonda de dissipação térmica na medida da transpiração de plantas jovens de lima ácida. Engenharia agrícola, Jaboticabal, v. 27, n. 2, p. 404-413, mai/ago., 2007| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - A |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 16. | | ANGELOCCI, L. R.; MARIN, F. R.; RIGHI, E. Z.; FAVARIN, J. L. Determinação do saldo de radiação de renque de cafeeiros em plantio semi-adensado. In: CONGRESSO BRASILEIRO DE AGROMETEOROLOGIA, 13, 2003, Santa Maria. Anais... Santa Maria: UNIFRA, SBA, UFSM, 2003. 75-76 folhas avulsas| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Territorial. |
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| 17. | | BERGAMASCHI, H.; VIEIRA, H. J.; OMETTO, J. C.; ANGELOCCI, L. R.; LIBARDI, P. L. Deficiência Hídrica em Feijoeiro. I. Análise de Crescimento e Fenologia. Pesquisa Agropecuária Brasileira, Brasília, v.23, n.7, p. 733-743, jul. 1988.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 18. | | BERGAMASCHI, H.; OMETTO, C.; VIEIRA, H. J.; ANGELOCCI, L. R.; LIBARDI, P. L. Deficiência Hídrica em Feijoeiro. II. Balanço de Energia. Pesquisa Agropecuária Brasileira, Brasília, v.23, n.7, p. 745-757, jul. 1988.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 19. | | BERGAMASCHI, H.; VIEIRA, H. J.; LIBARDI, P. L.; OMETTO, J. C.; ANGELOCCI, L. R. Deficiência Hídrica em Feijoeiro. III. Evapotranspiração Máxima e Relações com a Evapotranspiração Calculada pelo Método de Penman e com a Evaporação do Tanque "Calsse A" Pesquisa Agropecuária Brasileira, Brasília, v.24, n.4, p. 387-392,. abr. 1989| Biblioteca(s): Embrapa Unidades Centrais. |
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| 20. | | BERGAMASCHI, H.; VIEIRA, H. T. J.; OMETTO, J. C.; ANGELOCCI, L. R.; LIBARDI, P. Deficiência Hídrica em Feijoeiro. IV. Alterações Micrometeorológicas. Pesquisa Agropecuária Brasileira, Brasília, v.24, n.7, p. 769-777, jul. 1989| Biblioteca(s): Embrapa Unidades Centrais. |
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