Data/hora: 17/06/2024 23:35:13

Biblioteca(s): | Embrapa Gado de Leite.
Data corrente: | 06/02/2017
Data da última atualização: | 30/01/2023
Autoria: | SIQUEIRA, L. G. B.; HANSEN, P. J.
Afiliação: | LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; PETER J. HANSEN, University of Florida, Gainesville, Florida, USA.
Título: | Sex differences in response of the bovine embryo to colony-stimulating factor 2.
Ano de publicação: | 2016
Fonte/Imprenta: | Reproduction, n. 152, p. 645-654, 2016.
Idioma: | Inglês
Conteúdo: | We tested whether gene expression of the bovine morula is modified by CSF2 in a sex-dependent manner and if sex determines the effect of CSF2 on competence of embryos to become blastocysts. Embryos were produced in vitro using X- or Y-sorted semen and treated at Day 5 of culture with 10&#8201;ng/mL bovine CSF2 or control. In experiment 1, morulae were collected at Day 6 and biological replicates (n&#8201;=&#8201;8) were evaluated for transcript abundance of 90 genes by RT-qPCR using the Fluidigm Delta Gene assay. Expression of more than one-third (33 of 90) of genes examined was affected by sex. The effect of CSF2 on gene expression was modified by sex (P&#8201;<&#8201;0.05) for five genes (DDX3Y/DDX3X-like, NANOG, MYF6, POU5F1 and RIPK3) and tended (P&#8201;<&#8201;0.10) to be modified by sex for five other genes (DAPK1, HOXA5, PPP2R3A, PTEN and TNFSF8). In experiment 2, embryos were treated at Day 5 with control or CSF2 and blastocysts were collected at Day 7 for immunolabeling to determine the number of inner cell mass (ICM) and trophectoderm (TE) cells. CSF2 increased the percent of putative zygotes that became blastocysts for females, but did not affect the development of males. There was no effect of CSF2 or interaction of CSF2 with sex on the total number of blastomeres in blastocysts or in the number of inner cell mass or trophectoderm cells. In conclusion, CSF2 exerted divergent responses on gene expression and development of female and male embryos. These results are evidence of sexually dimorphic responses of the preimplantation embryo to this embryokine.
Palavras-Chave: | CSF2; Embryokine.
Categoria do assunto: | X Pesquisa, Tecnologia e Engenharia
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/154808/1/Cnpgl-2016-Reproduction-Sex.pdf
Registro original: | Embrapa Gado de Leite (CNPGL)
Exemplares: | CNPGL - (23106-1) - UPC - AP - DD -  -  -  - DIS