| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Gado de Leite. |
|
Data corrente: |
18/02/2014 |
|
Data da última atualização: |
05/02/2024 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
ARASHIRO, E. K. N.; PALHÃO, M. P.; WOHLRES-VIANA, S.; SIQUEIRA, L. G. B.; CAMARGO, L. S. de A.; HENRY, M. R. J. M.; VIANA, J. H. M. |
|
Afiliação: |
EDUARDO KENJI N. ARASHIRO, UFMG; MILLER PEREIRA PALHÃO, UNIFENAS; SABINE WOHLRES-VIANA, UFJF; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; MARC ROGER JEAN MARIE HENRY, UFMG; JOAO HENRIQUE MOREIRA VIANA, CNPGL. |
|
Título: |
In vivo collection of follicular fluid and granulosa cells from individual follicles of different diameters in cattle by an adapted ovum pick-up system. |
|
Ano de publicação: |
2013 |
|
Fonte/Imprenta: |
Reproductive Biology and Endocrinology, v. 11, article 73, 2013. |
|
DOI: |
https://doi.org/10.1186/1477-7827-11-73 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Background: Most studies on granulosa cell (GC) function in cattle have been performed using GC and follicular fluid (FF) samples collected from slaughterhouse ovaries. Using this approach, the follicular developmental stage and functional status are unknown and indirectly inferred, limiting data interpretation. Ultrasound-guided follicle aspiration has previously been used to recover GC or FF samples, but this was mostly carried out in large follicles or pools of small follicles, without recording the efficiency of recovery. The present study was aimed at adapting and evaluating an ovum pick-up (OPU) system for the in vivo recovery of FF and GC from individual follicles of different diameters. Methods: In the first trial, the losses of fluid inside the tubing system were calculated using a conventional or an adapted-OPU system. Blood plasma volumes equivalent to the amount of FF in follicles of different diameters were aspirated using a conventional OPU Teflon circuit. The OPU system was then adapted by connecting 0.25 mL straws to the circuit. A second trial evaluated the efficiency of FF recovery in vivo. Follicles ranging from 4.0 to 16.8 mm in diameter were aspirated individually using the conventional or adapted-OPU systems. A third trial assessed the in vivo recovery of GC and the subsequent amount of RNA obtained from the follicles of different diameters from Holstein and Gir cattle. Results: In Trial I, the plasma recovery efficiency was similar (P > 0.05) for the volumes expected for 12 and 10 mm follicles, but decreased (P < 0.05) for smaller follicles (45.7+/-4.0%, 12.4+/-4.3% and 0.0+/-0.0% for 8, 6, and 4 mm follicles, respectively). Using the adaptation, the losses intrinsic to the aspiration system were similar for all follicle diameters. In Trial II, the expected and recovered volumes of FF were correlated (r = 0.89) and the efficiency of recovery was similar among follicles <12 mm, while larger follicles had a progressive increase in FF losses that was not related to the tubing system. In Trial III, the number of GC and amount of RNA obtained were not affected (P > 0.05) by follicle size, but differed according to breed (615,054+/-58,122 vs 458,095+/-36,407 for Holstein and Gir, respectively; P < 0.05). Conclusions: The adapted-OPU system can be successfully used for the in vivo collection of FF and GC from follicles of different diameters. This will enable further endocrine, cellular, and gene expression analyses. MenosBackground: Most studies on granulosa cell (GC) function in cattle have been performed using GC and follicular fluid (FF) samples collected from slaughterhouse ovaries. Using this approach, the follicular developmental stage and functional status are unknown and indirectly inferred, limiting data interpretation. Ultrasound-guided follicle aspiration has previously been used to recover GC or FF samples, but this was mostly carried out in large follicles or pools of small follicles, without recording the efficiency of recovery. The present study was aimed at adapting and evaluating an ovum pick-up (OPU) system for the in vivo recovery of FF and GC from individual follicles of different diameters. Methods: In the first trial, the losses of fluid inside the tubing system were calculated using a conventional or an adapted-OPU system. Blood plasma volumes equivalent to the amount of FF in follicles of different diameters were aspirated using a conventional OPU Teflon circuit. The OPU system was then adapted by connecting 0.25 mL straws to the circuit. A second trial evaluated the efficiency of FF recovery in vivo. Follicles ranging from 4.0 to 16.8 mm in diameter were aspirated individually using the conventional or adapted-OPU systems. A third trial assessed the in vivo recovery of GC and the subsequent amount of RNA obtained from the follicles of different diameters from Holstein and Gir cattle. Results: In Trial I, the plasma recovery efficiency was similar (P > 0.05) for the v... Mostrar Tudo |
|
Palavras-Chave: |
Bovine; Follicle aspiration; Follicle biopsy. |
|
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
Marc: |
LEADER 03310naa a2200241 a 4500
001 1980418
005 2024-02-05
008 2013 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1186/1477-7827-11-73$2DOI
100 1 $aARASHIRO, E. K. N.
245 $aIn vivo collection of follicular fluid and granulosa cells from individual follicles of different diameters in cattle by an adapted ovum pick-up system.$h[electronic resource]
260 $c2013
520 $aBackground: Most studies on granulosa cell (GC) function in cattle have been performed using GC and follicular fluid (FF) samples collected from slaughterhouse ovaries. Using this approach, the follicular developmental stage and functional status are unknown and indirectly inferred, limiting data interpretation. Ultrasound-guided follicle aspiration has previously been used to recover GC or FF samples, but this was mostly carried out in large follicles or pools of small follicles, without recording the efficiency of recovery. The present study was aimed at adapting and evaluating an ovum pick-up (OPU) system for the in vivo recovery of FF and GC from individual follicles of different diameters. Methods: In the first trial, the losses of fluid inside the tubing system were calculated using a conventional or an adapted-OPU system. Blood plasma volumes equivalent to the amount of FF in follicles of different diameters were aspirated using a conventional OPU Teflon circuit. The OPU system was then adapted by connecting 0.25 mL straws to the circuit. A second trial evaluated the efficiency of FF recovery in vivo. Follicles ranging from 4.0 to 16.8 mm in diameter were aspirated individually using the conventional or adapted-OPU systems. A third trial assessed the in vivo recovery of GC and the subsequent amount of RNA obtained from the follicles of different diameters from Holstein and Gir cattle. Results: In Trial I, the plasma recovery efficiency was similar (P > 0.05) for the volumes expected for 12 and 10 mm follicles, but decreased (P < 0.05) for smaller follicles (45.7+/-4.0%, 12.4+/-4.3% and 0.0+/-0.0% for 8, 6, and 4 mm follicles, respectively). Using the adaptation, the losses intrinsic to the aspiration system were similar for all follicle diameters. In Trial II, the expected and recovered volumes of FF were correlated (r = 0.89) and the efficiency of recovery was similar among follicles <12 mm, while larger follicles had a progressive increase in FF losses that was not related to the tubing system. In Trial III, the number of GC and amount of RNA obtained were not affected (P > 0.05) by follicle size, but differed according to breed (615,054+/-58,122 vs 458,095+/-36,407 for Holstein and Gir, respectively; P < 0.05). Conclusions: The adapted-OPU system can be successfully used for the in vivo collection of FF and GC from follicles of different diameters. This will enable further endocrine, cellular, and gene expression analyses.
653 $aBovine
653 $aFollicle aspiration
653 $aFollicle biopsy
700 1 $aPALHÃO, M. P.
700 1 $aWOHLRES-VIANA, S.
700 1 $aSIQUEIRA, L. G. B.
700 1 $aCAMARGO, L. S. de A.
700 1 $aHENRY, M. R. J. M.
700 1 $aVIANA, J. H. M.
773 $tReproductive Biology and Endocrinology$gv. 11, article 73, 2013.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Leite (CNPGL) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 172 | |
| 121. |  | SIQUEIRA, L. G. B.; VIANA, J. H. M.; DINIZ, E. S.; CAMARGO, L. S. A.; AMORIM, L. S.; FONSECA, J. F.; FERNANDES, C. A. C.; TORRES, C. A. A. Aferição de ecogenicidade luteal com o uso de diferentes transdutores de ultra-som. Acta Scientiae Veterinariae, v. 34, supl. 1, p. s281, 2006. Edição dos resumos da 20. Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Araxá, MG, agosto 2006.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
|    |
| 122. | | CAMARGO, L. S. de A.; PEREIRA, M. M.; WOHLRES-VIANA, S.; QUINTAO, C. C. R.; IGUMA, L. T.; CARVALHO, B. C. de; SIQUEIRA, L. G. B.; VIANA, J. H. M. Altered gene expression in bovine somatic cell nuclear-transfered embryos after trichostatin a treatment. Reproduction, Fertility and Development, v. 24, n. 1, p. 121-122, 2011.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 123. | | SIQUEIRA, L. G. B.; VIANA, J. H. M.; TORRES, C. A. A.; SOUZA, E. D.; AMORIM, L. S.; OLIVEIRA, J. L.; CAMARGO, L. S. A.; Jr, P. L. J. M. Corpora lutea morphological and echotextural attributes related to plasma progesterona concentration in heifers. In: REPRODUCTION, FERTILITY AND DEVELOPMENT. 20. 2008, Anais. Colorado. Csiro, Academy of Science, 2008. 1 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 124. | | SIQUEIRA, L. G. B.; VIANA, J. H. M.; TORRES, C. A. A.; SOUZA, E. D.; AMORIM, L. S.; FONSECA, J. F. da; CAMARGO, L. S. A.; MONTEIRO JÚNIOR, P. L. J. Corpora lutea morphological and echotextural attributes related to plasma progesterone concentration in heifers. Reproduction, Fertility and Development, v. 20, n. 1, p. 237-238, 2008. Edição dos proceedings da Annual Conference or the International Embryo Transfer Society, Denver, Colorado, 2008.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
| |
| 125. | | MINARÉ, T. P.; ARASHIRO, E. K. N.; WOHLRES-VIANA, S.; SIQUEIRA, L. G. B.; PALHÃO, M. P.; CORDEIRO, C. E. C.; FERNANDES, C. A. C.; VIANA, J. H. M. Characterization of individual differences in the efficiency of superovulation in the Gyr breed. Animal Reproduction, v.10, n.3, p. 546, Jul./Sept. 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 126. | | SOUZA, J. F. da S. e; SOUZA, E. D. de; LEMOS, D. R. de; MUNK, M.; OLIVEIRA, C. S.; QUINTAO, C. C. R.; SARAIVA, N. Z.; SIQUEIRA, L. G. B.; CAMARGO, L. S. de A. Bovine oocyte and cumulus cells can exhibit distinct response to heat shock during in vitro maturation. Animal Reproduction, v. 20, n. 2, 2023. Abstracts of 36th Annual Meeting of the Brazilian Embryo Technology Society (SBTE).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 127. | | AMORIM, L. S.; TORRES, C. A. A.; AMORIM, E. A. M.; GUIMARÃES, J. D.; SILVA FILHO, J. M.; FONSECA, J. F. da; VIANA, J. H. M.; SIQUEIRA, L. G. B. Características físicas e morfológicas do sêmen de touros da raça nelore confinados e tratados com somatotrofina bovina recombinante (r-bST). Acta Scientiae Veterinariae, Porto Alegre, v. 34, supl. 1, p. s377, 2006. Edição dos resumos da 20. Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Araxá, MG, agosto 2006.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
| |
| 128. | | AMORIM, L. da S.; TORRES, C. A. A.; SIQUEIRA, L. G. B.; FONSECA, J. F. da; GUIMARÃES, J. D.; CARVALHO, G. R. de; ALVES, N. G.; OLIVEIRA, M. M. N. F. de. Embryo development and follicular status of Toggenburg does fed urea diet. Revista Brasileira de Zootecnia, v. 40, n. 2, p. 277-285, 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
| |
| 129. | | DIAS, J. H.; GONCALVES, J. D.; ARRAIS, A. M.; SOUZA-FABJAN, J. M. G.; BASTOS R.; BATISTA, R. I. T. P.; SIQUEIRA, L. G. B.; OLIVEIRA, M. E. F.; FONSECA, J. F. da. Effects of different doses of estradiol benzoate used in a cervical relaxation protocol on the success of non-surgical embryo recovery and luteal function in superovulated ewes. Domestic Animal Endocrinology, v. 82, 106751, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
|  |
| 130. | | SOUZA, E. D. de; PAULA, F. B. E.; QUINTAO, C. C. R.; VIANA, J. H. M.; SIQUEIRA, L. G. B.; IGUMA, L. T.; CARVALHO, B. C. de; LOURO, I. D.; CAMARGO, L. S. de A. Effect of HSP90 inibitor on developmental competence of bovine oocytes. Animal Reproduction, v.10, n.3, p. 515, Jul./Sept. 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 131. | | ARASHIRO, E. K. N.; WOHLRES-VIANA, S.; PALHÃO, M. P.; RABELO, N. A.; CAMARGO, L. S. de A.; SIQUEIRA, L. G. B.; PAIM, C. A. S.; VIANA, J. H. M. Follicular fluid and granulosa cell recovery from bovine follicles of different diameters using an adapted transvaginal-guided follicular aspiration system. In: ANNUAL CONFERENCE OF THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 38., 2012, Phoenix, Arizona. Abstracts... [S.l.: s.n.], 2012.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 132. | | CARVALHEIRA, L. R.; CARVALHO, B. C. de; RIBEIRO, L. S.; GOES, T. J. F.; VIEIRA, S. F.; FONTES, W. F.; TORRES FILHO, R. A.; OLIVEIRA, C. S.; SIQUEIRA, L. G. B.; CAMARGO, L. S. de A.; BRANDÃO, F. Z. Follicular population and oocyte quality from Holstein x Gyr cows under heat stress in climatic chamber and supplemented with organic chromium. Animal Reproduction, v. 15, n. 3, p. 403, 2018. Edição dos proceedings do 32º Annual Meeting of the Brazilian Embryo Technology Society, Florianópolis, 2018.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 133. | | FERRÃO, F. R.; SILVA, F. B. da; SCHWARZ, D. G. G.; OLIVEIRA, C. T. S. A. M. DE; SIQUEIRA, L. G. B.; BLUME, G. R.; COSTA, P. M.; MARTINS, C. F. Recuperação e avaliação de espermatozóides obtidos de epidídimos bovinos resfriados por diferentes períodos (2009). Planaltina, DF: Embrapa Cerrados, 2009. 1 folder.| Tipo: Folder/Folheto/Cartilha |
| Biblioteca(s): Embrapa Cerrados. |
| |
| 134. | | FERRÃO, F. R.; SILVA, F. B. da; SCHWARZ, D. G. G.; OLIVEIRA, C. T. S. A. M.; SIQUEIRA, L. G. B.; BLUME, G. R.; COSTA, P. M.; MARTINS, C. F. Recuperação e avaliação de espermatozóides obtidos de epidídimos bovinos resfriados por diferentes períodos. In: ENCONTRO DE JOVENS TALENTOS DA EMBRAPA CERRADOS, 4., 2009, Planaltina, DF. Resumos apresentados... Planaltina, DF: Embrapa Cerrados, 2009. p. 15-16 (Embrapa Cerrados. Documentos, 243).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Cerrados. |
| |
| 135. | | SIQUEIRA, L. G. B.; TRIBULO, P.; CHEN, Z.; DENICOL, A. C.; ORTEGA, M. S.; NEGRON-PEREZ, V.M.; KANNAMPUZHA-FRANCIS, J. P.; POHLER, K. G.; RIVERA, R. M.; HANSEN, P. J. Colony-stimulating factor 2 acts from days 5 to 7 of development to modify programming of the bovine conceptus at day 86 of gestation. Biology of Reproduction, v. 96, n. 4, p. 743-757, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 136. | | MAIA, A. L. R. S.; BRANDÃO, F. Z.; SOUZA-FABJAN, J. M. G.; VEIGA, M. O.; BALARO, M. F. A.; SIQUEIRA, L. G. B.; FACO, O.; FONSECA, J. F. da. Hydrometra in dairy goats: ultrasonic variables and therapeutic protocols evaluated during the reproductive season. Animal Reproduction Science, v. 197, p. 203-211, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
| |
| 137. | | MALARD, P. F.; PEIXER, M. A. S.; GRAZIA, J. G.; BRUNEL, H. dos S. S.; FERES, L. F.; VILLARROEL, C. L.; SIQUEIRA, L. G. B.; DODE, M. A. N.; POGUE, R.; VIANA, J. H. M.; CARVALHO, J. L. Intraovarian injection of mesenchymal stem cells improves oocyte yield and in vitro embryo production in a bovine model of fertility loss. Scientific Reports, v. 10, article 8018, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 138. | | VIANA, J. H. M.; ARASHIRO, E. K. N.; SIQUEIRA, L. G. B.; GHETTI, A. M.; AREAS, V. S.; GUIMARÃES, C. R. B.; PALHAO, M. P.; CAMARGO, L. S. de A.; FERNANDES, C. A. C. Doppler ultrasonography as a tool for ovarian management. Animal Reproduction, v. 10, n. 3, p. 215-222, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 139. | | CARVALHEIRA, L. R.; BRANDÃO, F. Z.; GOES, T. J. F.; QUINTAO, C. C. R.; OLIVEIRA, C. S.; SIQUEIRA, L. G. B.; CAMARGO, L. S. de A.; TORRES FILHO, R. A.; CARVALHO, B. C. de. DNA fragmentation in oocytes of Holstein x Gyr cows supplemented with organic chromium under heat stress in climatic chamber. Animal Reproduction, v. 15, n. 3, p. 418, 2018. Edição dos proceedings do 32º Annual Meeting of the Brazilian Embryo Technology Society, Florianópolis, 2018.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 140. | | MENDONÇA, J. F. M.; BRITO, M. A. V. P. e; LANGE, C. C.; SILVA, M. R.; MENDONCA, L. C.; RIBEIRO, J. B.; SIQUEIRA, L. G. B.; SIAS, G. R. F. V.; SOARES, L. A. P.; SOUZA, G. N. de. Decision-making in mastitis prevention and control at regional, herd and individual levels based on epidemiological and economic studies. Journal of Veterinary Science & Animal Husbandry, v. 6, n. 5, article 502, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: C - 0 |
| Biblioteca(s): Embrapa Gado de Leite. |
| |
| Registros recuperados : 172 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
