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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
06/02/2017 |
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Data da última atualização: |
30/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SIQUEIRA, L. G. B.; HANSEN, P. J. |
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Afiliação: |
LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; PETER J. HANSEN, University of Florida, Gainesville, Florida, USA. |
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Título: |
Sex differences in response of the bovine embryo to colony-stimulating factor 2. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Reproduction, n. 152, p. 645-654, 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
We tested whether gene expression of the bovine morula is modified by CSF2 in a sex-dependent manner and if sex determines the effect of CSF2 on competence of embryos to become blastocysts. Embryos were produced in vitro using X- or Y-sorted semen and treated at Day 5 of culture with 10 ng/mL bovine CSF2 or control. In experiment 1, morulae were collected at Day 6 and biological replicates (n = 8) were evaluated for transcript abundance of 90 genes by RT-qPCR using the Fluidigm Delta Gene assay. Expression of more than one-third (33 of 90) of genes examined was affected by sex. The effect of CSF2 on gene expression was modified by sex (P < 0.05) for five genes (DDX3Y/DDX3X-like, NANOG, MYF6, POU5F1 and RIPK3) and tended (P < 0.10) to be modified by sex for five other genes (DAPK1, HOXA5, PPP2R3A, PTEN and TNFSF8). In experiment 2, embryos were treated at Day 5 with control or CSF2 and blastocysts were collected at Day 7 for immunolabeling to determine the number of inner cell mass (ICM) and trophectoderm (TE) cells. CSF2 increased the percent of putative zygotes that became blastocysts for females, but did not affect the development of males. There was no effect of CSF2 or interaction of CSF2 with sex on the total number of blastomeres in blastocysts or in the number of inner cell mass or trophectoderm cells. In conclusion, CSF2 exerted divergent responses on gene expression and development of female and male embryos. These results are evidence of sexually dimorphic responses of the preimplantation embryo to this embryokine. MenosWe tested whether gene expression of the bovine morula is modified by CSF2 in a sex-dependent manner and if sex determines the effect of CSF2 on competence of embryos to become blastocysts. Embryos were produced in vitro using X- or Y-sorted semen and treated at Day 5 of culture with 10 ng/mL bovine CSF2 or control. In experiment 1, morulae were collected at Day 6 and biological replicates (n = 8) were evaluated for transcript abundance of 90 genes by RT-qPCR using the Fluidigm Delta Gene assay. Expression of more than one-third (33 of 90) of genes examined was affected by sex. The effect of CSF2 on gene expression was modified by sex (P < 0.05) for five genes (DDX3Y/DDX3X-like, NANOG, MYF6, POU5F1 and RIPK3) and tended (P < 0.10) to be modified by sex for five other genes (DAPK1, HOXA5, PPP2R3A, PTEN and TNFSF8). In experiment 2, embryos were treated at Day 5 with control or CSF2 and blastocysts were collected at Day 7 for immunolabeling to determine the number of inner cell mass (ICM) and trophectoderm (TE) cells. CSF2 increased the percent of putative zygotes that became blastocysts for females, but did not affect the development of males. There was no effect of CSF2 or interaction of CSF2 with sex on the total number of blastomeres in blastocysts or in the number of inner cell mass or trophectoderm cells. In conclusion, CSF2 exerted divergent responses on gene expression and development of female and male embryos. These results a... Mostrar Tudo |
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Palavras-Chave: |
CSF2; Embryokine. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/154808/1/Cnpgl-2016-Reproduction-Sex.pdf
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Marc: |
LEADER 02045naa a2200157 a 4500
001 2062824
005 2023-01-30
008 2016 bl uuuu u00u1 u #d
100 1 $aSIQUEIRA, L. G. B.
245 $aSex differences in response of the bovine embryo to colony-stimulating factor 2.$h[electronic resource]
260 $c2016
520 $aWe tested whether gene expression of the bovine morula is modified by CSF2 in a sex-dependent manner and if sex determines the effect of CSF2 on competence of embryos to become blastocysts. Embryos were produced in vitro using X- or Y-sorted semen and treated at Day 5 of culture with 10 ng/mL bovine CSF2 or control. In experiment 1, morulae were collected at Day 6 and biological replicates (n = 8) were evaluated for transcript abundance of 90 genes by RT-qPCR using the Fluidigm Delta Gene assay. Expression of more than one-third (33 of 90) of genes examined was affected by sex. The effect of CSF2 on gene expression was modified by sex (P < 0.05) for five genes (DDX3Y/DDX3X-like, NANOG, MYF6, POU5F1 and RIPK3) and tended (P < 0.10) to be modified by sex for five other genes (DAPK1, HOXA5, PPP2R3A, PTEN and TNFSF8). In experiment 2, embryos were treated at Day 5 with control or CSF2 and blastocysts were collected at Day 7 for immunolabeling to determine the number of inner cell mass (ICM) and trophectoderm (TE) cells. CSF2 increased the percent of putative zygotes that became blastocysts for females, but did not affect the development of males. There was no effect of CSF2 or interaction of CSF2 with sex on the total number of blastomeres in blastocysts or in the number of inner cell mass or trophectoderm cells. In conclusion, CSF2 exerted divergent responses on gene expression and development of female and male embryos. These results are evidence of sexually dimorphic responses of the preimplantation embryo to this embryokine.
653 $aCSF2
653 $aEmbryokine
700 1 $aHANSEN, P. J.
773 $tReproduction$gn. 152, p. 645-654, 2016.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 172 | |
| 4. |  | SIQUEIRA, L. G. B.; HANSEN, P. J. Postnatal consequences of assisted reproductive technologies in cattle. Animal Reproduction, v. 14, n. 3, p. 490-496, 2017. Animal Reproduction, v. 14, n. 3, p. 490-496, 2017.
Edição dos proceedings do Annual Meeting of the Brazilian Embryo Tecnology Society, Cabo de Santo Agostinho, 2017.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 6. | | TRIBULO, P.; SIQUEIRA, L. G. B.; HANSEN, P. J. Endometrial expression of genes involved in growth factor, cytoline, hormone, and WNT signaling during the early estrous cycle of the cow In: ANNUAL MEETING OF THE SOCIETY FOR THE STUDY OF REPRODUCTION, 48., 2015, San Juan, Puerto Rico, USA. Evolution of sex: abstracts. San Juan: SSR, 2015. P. 113.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 13. | | VIANA, J. H. M.; FIGUEIREDO, A. C. S.; SIQUEIRA, L. G. B. Brazilian embryo industry in context: pitfalls, lessons, and expectations for the future. Animal Reproduction, v. 14, n. 3, p. 476-481, 2017. Edição dos proceedings do 31º Annual Meeting of the Brazilian Embryo Technology Society, 2017, Cabo de Santo Agostinho.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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| 19. | | LESSARD, C.; SIQUEIRA, L. G. B.; D'AMOURS, O.; SULLIVAN, R.; LECLERC, P.; PALMER, C. Infertility in a beef bull due to a failure in the capacitation process. Theriogenology, v. 76, n. 5, p. 891-899, 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 20. | | CUNHA, E. R.; SILVA, C. G.; SIQUEIRA, L. G. B.; MARTINS, C. F. Isolation, culture and cryopreservation of bovine amniotic cells with Dimethyl sulfoxide, Dimethylformamide or Glycerol. Animal Reproduction, v. 7, n. 3, p. 317, Jul./Sept. 2010. Edição dos resumos do III International Symposium Animal Biology of Reproductive, 3., 2010, Águas de São Pedro.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Cerrados. |
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| Registros recuperados : 172 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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