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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
16/11/2018 |
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Data da última atualização: |
06/04/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CONCEIÇÃO, A. A.; SOARES NETO, C. B.; RIBEIRO, J. A. de A.; SIQUEIRA, F. G. de; MILLER, R. N. G.; MENDONCA, S. |
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Afiliação: |
APARECIDO ALMEIDA CONCEIÇÃO, Universidade Federal da Bahia; CLEMENTE BATISTA SOARES NETO, Universidade de Brasília; JOSE ANTONIO DE AQUINO RIBEIRO, CNPAE; FELIX GONCALVES DE SIQUEIRA, CNPAE; ROBERT NEIL GERARD MILLER, Universidade de Brasília; SIMONE MENDONCA, CNPAE. |
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Título: |
Development of an RP-UHPLC-PDA method for quantification of free gossypol in cottonseed cake and fungal-treated cottonseed cake. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Plos One, v. 13, n. 5, e0196164, May 2018. |
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DOI: |
https://doi.org/10.1371/journal.pone.0196164 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Cottonseed cake biomass, which is a residue of oil extraction, is potentially appropriate for use as animal feed, given the high mineral, fibre and protein content. The presence of free gossypol, however, a toxic pigment in the glands of the cotton plant, limits use of this biomass for monogastric livestock. A promising method to detoxify cottonseed cake relies on fermentation by fungi, which can eliminate up to 100% of gossypol. In order to quantify trace levels of free gossypol in different cotton materials, including cottonseed cake treated with macrofungi, a simple and rapid chromatographic detection method was developed and validated. Under optimized conditions, extraction was performed using 70% acetone. The extract was then analysed by Ultra High-Performance Liquid Chromatography (UHPLC), with gradient elution on a C18 reverse phase column KINETEX® (100 x 2.10 mm, 2.6 um). Methanol-0.1% TFA aqueous solution was employed as mobile phase and PDA detection conducted at 254 nm. The optimized method was validated by analysis of specificity, linearity and range, limit of detection, limit of quantification, precision and accuracy. Detection and quantification limits were observed at 0.2 and 0.5 ug/mL, respectively. With good reproducibility, with precision (RSD)<10% and recovery greater than 94%, the developed assay was appropriate for quantification of low quantities of free gossypol. The validated method was successfully applied to determine trace levels of free gossypol cottonseed treated with a macrofungus. MenosAbstract: Cottonseed cake biomass, which is a residue of oil extraction, is potentially appropriate for use as animal feed, given the high mineral, fibre and protein content. The presence of free gossypol, however, a toxic pigment in the glands of the cotton plant, limits use of this biomass for monogastric livestock. A promising method to detoxify cottonseed cake relies on fermentation by fungi, which can eliminate up to 100% of gossypol. In order to quantify trace levels of free gossypol in different cotton materials, including cottonseed cake treated with macrofungi, a simple and rapid chromatographic detection method was developed and validated. Under optimized conditions, extraction was performed using 70% acetone. The extract was then analysed by Ultra High-Performance Liquid Chromatography (UHPLC), with gradient elution on a C18 reverse phase column KINETEX® (100 x 2.10 mm, 2.6 um). Methanol-0.1% TFA aqueous solution was employed as mobile phase and PDA detection conducted at 254 nm. The optimized method was validated by analysis of specificity, linearity and range, limit of detection, limit of quantification, precision and accuracy. Detection and quantification limits were observed at 0.2 and 0.5 ug/mL, respectively. With good reproducibility, with precision (RSD)<10% and recovery greater than 94%, the developed assay was appropriate for quantification of low quantities of free gossypol. The validated method was successfully applied to determine trace levels of free ... Mostrar Tudo |
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Palavras-Chave: |
Human-serum albumin; Performance liquid-chromatography; Terpenoid aldehydes. |
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Thesaurus Nal: |
Biomass; Cottonseed cake; Gossypol; White-rot fungi. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/222356/1/pone.0196164.pdf
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Marc: |
LEADER 02441naa a2200277 a 4500
001 2099518
005 2021-04-06
008 2018 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1371/journal.pone.0196164$2DOI
100 1 $aCONCEIÇÃO, A. A.
245 $aDevelopment of an RP-UHPLC-PDA method for quantification of free gossypol in cottonseed cake and fungal-treated cottonseed cake.$h[electronic resource]
260 $c2018
520 $aAbstract: Cottonseed cake biomass, which is a residue of oil extraction, is potentially appropriate for use as animal feed, given the high mineral, fibre and protein content. The presence of free gossypol, however, a toxic pigment in the glands of the cotton plant, limits use of this biomass for monogastric livestock. A promising method to detoxify cottonseed cake relies on fermentation by fungi, which can eliminate up to 100% of gossypol. In order to quantify trace levels of free gossypol in different cotton materials, including cottonseed cake treated with macrofungi, a simple and rapid chromatographic detection method was developed and validated. Under optimized conditions, extraction was performed using 70% acetone. The extract was then analysed by Ultra High-Performance Liquid Chromatography (UHPLC), with gradient elution on a C18 reverse phase column KINETEX® (100 x 2.10 mm, 2.6 um). Methanol-0.1% TFA aqueous solution was employed as mobile phase and PDA detection conducted at 254 nm. The optimized method was validated by analysis of specificity, linearity and range, limit of detection, limit of quantification, precision and accuracy. Detection and quantification limits were observed at 0.2 and 0.5 ug/mL, respectively. With good reproducibility, with precision (RSD)<10% and recovery greater than 94%, the developed assay was appropriate for quantification of low quantities of free gossypol. The validated method was successfully applied to determine trace levels of free gossypol cottonseed treated with a macrofungus.
650 $aBiomass
650 $aCottonseed cake
650 $aGossypol
650 $aWhite-rot fungi
653 $aHuman-serum albumin
653 $aPerformance liquid-chromatography
653 $aTerpenoid aldehydes
700 1 $aSOARES NETO, C. B.
700 1 $aRIBEIRO, J. A. de A.
700 1 $aSIQUEIRA, F. G. de
700 1 $aMILLER, R. N. G.
700 1 $aMENDONCA, S.
773 $tPlos One$gv. 13, n. 5, e0196164, May 2018.
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Embrapa Agroenergia (CNPAE) |
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| 4113. |  | SILVA, H. D. da; PIRES, I. E.; RIBASKI, J.; DRUMOND, M. A.; LIMA, P. C. F.; SOUZA, S. M. de; FERREIRA, C. A. Comportamento de essências florestais nas regiões árida e semi-árida do Nordeste: resultados preliminares. Brasilia, DF: EMBRAPA-DID, 1980. 25 p. (EMBRAPA-DTC. Documentos, 1),| Biblioteca(s): Embrapa Semiárido; Embrapa Unidades Centrais. |
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