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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
16/11/2018 |
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Data da última atualização: |
06/04/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CONCEIÇÃO, A. A.; SOARES NETO, C. B.; RIBEIRO, J. A. de A.; SIQUEIRA, F. G. de; MILLER, R. N. G.; MENDONCA, S. |
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Afiliação: |
APARECIDO ALMEIDA CONCEIÇÃO, Universidade Federal da Bahia; CLEMENTE BATISTA SOARES NETO, Universidade de Brasília; JOSE ANTONIO DE AQUINO RIBEIRO, CNPAE; FELIX GONCALVES DE SIQUEIRA, CNPAE; ROBERT NEIL GERARD MILLER, Universidade de Brasília; SIMONE MENDONCA, CNPAE. |
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Título: |
Development of an RP-UHPLC-PDA method for quantification of free gossypol in cottonseed cake and fungal-treated cottonseed cake. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Plos One, v. 13, n. 5, e0196164, May 2018. |
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DOI: |
https://doi.org/10.1371/journal.pone.0196164 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Cottonseed cake biomass, which is a residue of oil extraction, is potentially appropriate for use as animal feed, given the high mineral, fibre and protein content. The presence of free gossypol, however, a toxic pigment in the glands of the cotton plant, limits use of this biomass for monogastric livestock. A promising method to detoxify cottonseed cake relies on fermentation by fungi, which can eliminate up to 100% of gossypol. In order to quantify trace levels of free gossypol in different cotton materials, including cottonseed cake treated with macrofungi, a simple and rapid chromatographic detection method was developed and validated. Under optimized conditions, extraction was performed using 70% acetone. The extract was then analysed by Ultra High-Performance Liquid Chromatography (UHPLC), with gradient elution on a C18 reverse phase column KINETEX® (100 x 2.10 mm, 2.6 um). Methanol-0.1% TFA aqueous solution was employed as mobile phase and PDA detection conducted at 254 nm. The optimized method was validated by analysis of specificity, linearity and range, limit of detection, limit of quantification, precision and accuracy. Detection and quantification limits were observed at 0.2 and 0.5 ug/mL, respectively. With good reproducibility, with precision (RSD)<10% and recovery greater than 94%, the developed assay was appropriate for quantification of low quantities of free gossypol. The validated method was successfully applied to determine trace levels of free gossypol cottonseed treated with a macrofungus. MenosAbstract: Cottonseed cake biomass, which is a residue of oil extraction, is potentially appropriate for use as animal feed, given the high mineral, fibre and protein content. The presence of free gossypol, however, a toxic pigment in the glands of the cotton plant, limits use of this biomass for monogastric livestock. A promising method to detoxify cottonseed cake relies on fermentation by fungi, which can eliminate up to 100% of gossypol. In order to quantify trace levels of free gossypol in different cotton materials, including cottonseed cake treated with macrofungi, a simple and rapid chromatographic detection method was developed and validated. Under optimized conditions, extraction was performed using 70% acetone. The extract was then analysed by Ultra High-Performance Liquid Chromatography (UHPLC), with gradient elution on a C18 reverse phase column KINETEX® (100 x 2.10 mm, 2.6 um). Methanol-0.1% TFA aqueous solution was employed as mobile phase and PDA detection conducted at 254 nm. The optimized method was validated by analysis of specificity, linearity and range, limit of detection, limit of quantification, precision and accuracy. Detection and quantification limits were observed at 0.2 and 0.5 ug/mL, respectively. With good reproducibility, with precision (RSD)<10% and recovery greater than 94%, the developed assay was appropriate for quantification of low quantities of free gossypol. The validated method was successfully applied to determine trace levels of free ... Mostrar Tudo |
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Palavras-Chave: |
Human-serum albumin; Performance liquid-chromatography; Terpenoid aldehydes. |
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Thesaurus Nal: |
Biomass; Cottonseed cake; Gossypol; White-rot fungi. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/222356/1/pone.0196164.pdf
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Marc: |
LEADER 02441naa a2200277 a 4500
001 2099518
005 2021-04-06
008 2018 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1371/journal.pone.0196164$2DOI
100 1 $aCONCEIÇÃO, A. A.
245 $aDevelopment of an RP-UHPLC-PDA method for quantification of free gossypol in cottonseed cake and fungal-treated cottonseed cake.$h[electronic resource]
260 $c2018
520 $aAbstract: Cottonseed cake biomass, which is a residue of oil extraction, is potentially appropriate for use as animal feed, given the high mineral, fibre and protein content. The presence of free gossypol, however, a toxic pigment in the glands of the cotton plant, limits use of this biomass for monogastric livestock. A promising method to detoxify cottonseed cake relies on fermentation by fungi, which can eliminate up to 100% of gossypol. In order to quantify trace levels of free gossypol in different cotton materials, including cottonseed cake treated with macrofungi, a simple and rapid chromatographic detection method was developed and validated. Under optimized conditions, extraction was performed using 70% acetone. The extract was then analysed by Ultra High-Performance Liquid Chromatography (UHPLC), with gradient elution on a C18 reverse phase column KINETEX® (100 x 2.10 mm, 2.6 um). Methanol-0.1% TFA aqueous solution was employed as mobile phase and PDA detection conducted at 254 nm. The optimized method was validated by analysis of specificity, linearity and range, limit of detection, limit of quantification, precision and accuracy. Detection and quantification limits were observed at 0.2 and 0.5 ug/mL, respectively. With good reproducibility, with precision (RSD)<10% and recovery greater than 94%, the developed assay was appropriate for quantification of low quantities of free gossypol. The validated method was successfully applied to determine trace levels of free gossypol cottonseed treated with a macrofungus.
650 $aBiomass
650 $aCottonseed cake
650 $aGossypol
650 $aWhite-rot fungi
653 $aHuman-serum albumin
653 $aPerformance liquid-chromatography
653 $aTerpenoid aldehydes
700 1 $aSOARES NETO, C. B.
700 1 $aRIBEIRO, J. A. de A.
700 1 $aSIQUEIRA, F. G. de
700 1 $aMILLER, R. N. G.
700 1 $aMENDONCA, S.
773 $tPlos One$gv. 13, n. 5, e0196164, May 2018.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Acre. |
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Data corrente: |
28/07/2016 |
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Data da última atualização: |
02/07/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
COSTA, D. A. da; ALVARES, V. de S.; NOGUEIRA, R. M.; KUSDRA, J. F.; MACIEL, V. T.; MIQUELONI, D. P. |
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Afiliação: |
David Aquino da Costa, Universidade Federal do Acre; VIRGINIA DE SOUZA ALVARES, CPAF-Acre; Roberta Martins Nogueira, Universidade Federal do Mato Grosso; Jorge Ferreira Kusdra, Universidade Federal do Acre; VLAYRTON TOME MACIEL, CPAF-Acre; Daniela Popim Miqueloni, Universidade Federal do Acre. |
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Título: |
Quality of Brazil nuts stored in forced aeration silos. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Revista Ceres, Viçosa, MG, v. 63, n. 3, p. 305-314, maio/jun. 2016. |
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ISSN: |
2177-3491 |
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DOI: |
10.1590/0034-737X201663030005 |
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Idioma: |
Inglês |
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Conteúdo: |
The traditional system of collection and storage of Brazil nut compromises seriously the quality of these almonds as it contributes to the high incidence of contaminants, like fungi of the genus Aspergillus, which can produce aflatoxins. In this study, the objective was to evaluate the influence of the storage period in studied conditions, on the physicochemical characteristics and on the microbiological contamination of Brazil nuts. The experimental was designed as completely randomized, considering as treatments the storage period (0 - control, 30, 60, 90, 120 and 150 days) with four replicates of 3 kg of Brazil nuts each. The samples were submitted to physicochemical and microbiological analysis. It was observed that almonds submitted to the storage had their moisture content reduced by 78.2% at 150 days of storage, however, this reduction was not fast enough to avoid surface contamination by filamentous and potentially aflatoxins producing fungi. The critical period of contamination occurred on the first 30 days of storage when there was an increase of the studied fungi, as well as B1 and total aflatoxin. The studied storage conditions were four times more effective in reducing the product moisture content than the traditional methods, however, pre-drying is necessary to avoid contamination of the product. |
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Palavras-Chave: |
Aeration silos; Aflatoxinas; Calidad de almacenaje; Calidad de la fruta; Castanha do brasil; Enfermedades postcosecha; Nuez del Brasil. |
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Thesagro: |
Aflatoxina; Armazenamento; Bertholletia excelsa; Castanha do pará; Contaminação fúngica; Doença de planta; Fruto; Fungo; Qualidade; Silo aéreo. |
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Thesaurus NAL: |
Aflatoxins; Aspergillus; Brazil nuts; Fruit quality; Fungi; Postharvest diseases; Silos; Storage quality. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/145911/1/26097.pdf
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Marc: |
LEADER 02706naa a2200505 a 4500
001 2049870
005 2021-07-02
008 2016 bl uuuu u00u1 u #d
022 $a2177-3491
024 7 $a10.1590/0034-737X201663030005$2DOI
100 1 $aCOSTA, D. A. da
245 $aQuality of Brazil nuts stored in forced aeration silos.$h[electronic resource]
260 $c2016
520 $aThe traditional system of collection and storage of Brazil nut compromises seriously the quality of these almonds as it contributes to the high incidence of contaminants, like fungi of the genus Aspergillus, which can produce aflatoxins. In this study, the objective was to evaluate the influence of the storage period in studied conditions, on the physicochemical characteristics and on the microbiological contamination of Brazil nuts. The experimental was designed as completely randomized, considering as treatments the storage period (0 - control, 30, 60, 90, 120 and 150 days) with four replicates of 3 kg of Brazil nuts each. The samples were submitted to physicochemical and microbiological analysis. It was observed that almonds submitted to the storage had their moisture content reduced by 78.2% at 150 days of storage, however, this reduction was not fast enough to avoid surface contamination by filamentous and potentially aflatoxins producing fungi. The critical period of contamination occurred on the first 30 days of storage when there was an increase of the studied fungi, as well as B1 and total aflatoxin. The studied storage conditions were four times more effective in reducing the product moisture content than the traditional methods, however, pre-drying is necessary to avoid contamination of the product.
650 $aAflatoxins
650 $aAspergillus
650 $aBrazil nuts
650 $aFruit quality
650 $aFungi
650 $aPostharvest diseases
650 $aSilos
650 $aStorage quality
650 $aAflatoxina
650 $aArmazenamento
650 $aBertholletia excelsa
650 $aCastanha do pará
650 $aContaminação fúngica
650 $aDoença de planta
650 $aFruto
650 $aFungo
650 $aQualidade
650 $aSilo aéreo
653 $aAeration silos
653 $aAflatoxinas
653 $aCalidad de almacenaje
653 $aCalidad de la fruta
653 $aCastanha do brasil
653 $aEnfermedades postcosecha
653 $aNuez del Brasil
700 1 $aALVARES, V. de S.
700 1 $aNOGUEIRA, R. M.
700 1 $aKUSDRA, J. F.
700 1 $aMACIEL, V. T.
700 1 $aMIQUELONI, D. P.
773 $tRevista Ceres, Viçosa, MG$gv. 63, n. 3, p. 305-314, maio/jun. 2016.
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Embrapa Acre (CPAF-AC) |
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