Data/hora: 04/06/2024 18:01:57

Biblioteca(s): | Embrapa Agroenergia.
Data corrente: | 04/05/2019
Data da última atualização: | 18/11/2019
Autoria: | SCHNEIDER, W. D. H.; COSTA, P. C.; FONTANA, R. C.; SIQUEIRA, F. G. de; DILLON, A. J. P.; CAMASSOLA, M.
Afiliação: | William Daniel Hahn Schneider, Universidade de Caxias do Sul; Paula Cavion Costa, Universidade de Caxias do Sul; Roselei Claudete Fontana, Universidade de Caxias do Sul; FELIX GONCALVES DE SIQUEIRA, CNPAE; Aldo José Pinheiro Dillon, Universidade de Caxias do Sul; Marli Camassola, Universidade de Caxias do Sul.
Título: | Upscale and characterization of lignin-modifying enzymes from Marasmiellus palmivorus VE111 in a bioreactor under parameter optimization and the effect of inducers.
Ano de publicação: | 2019
Fonte/Imprenta: | Journal of Biotechnology, v. 295, p. 1-8.
DOI: | https://doi.org/10.1016/j.jbiotec.2019.03.002
Idioma: | Inglês
Conteúdo: | Testing different pHs, dissolved oxygen concentrations and temperatures, plus the addition of inducers, to optimize ligninolytic enzyme activity, resulted in increased production of laccases, total peroxidases and manganese peroxidases on the order of 2.1-fold, 4.6-fold and 10-fold, respectively; laccases reached 6588&#8201;U/mL, total peroxidases reached 3533&#8201;U/mL and manganese peroxidase achieved 60&#8201;U/mL. Furthermore, an increase in laccase volumetric productivity and in its specific activity was verified. The addition of inducers, such as copper sulphate and manganese sulphate, improved enzymatic activity. In addition, a new previously unidentified laccase isoform was documented by zymography. The present work successfully increased the production of ligninolytic enzymes.
Palavras-Chave: | Copper sulphate; Laccases; White-rot basidiomycete.
Thesaurus Nal: | Lignin; Peroxidases.
Categoria do assunto: | --
Registro original: | Embrapa Agroenergia (CNPAE)
Exemplares: | CNPAE - (3459-1) - UPC - AP - DD -  -  -  - DIS