Data/hora: 21/06/2024 11:56:47

Biblioteca(s): 	Embrapa Agricultura Digital.	
Data corrente: 	28/08/2007	
Data da última atualização: 	17/01/2020	
Autoria: 	SIMÕES, M.; BAHIA, D.; ZERLOTINI, A.; TORRES, K.; ARTIGUENAVE, F.; NESHICH, G.; KUSER, P.; OLIVEIRA, G.	
Afiliação: 	MARIANA SIMÕES, Fiocruz; DIANA BAHIA, Fiocruz; ADHEMAR ZERLOTINI, Fiocruz; KLEIDER TORRES, Fiocruz; FRANÇOIS ARTIGUENAVE, Inra; GORAN NESHICH, CNPTIA; PAULA REGINA KUSER FALCAO, CNPTIA; GUILHERME OLIVEIRA, Fiocruz, Santa Casa de Belo Horizonte.	
Título: 	Single nucleotide polymorphisms identification in expressed genes of Schistosoma mansoni.	
Ano de publicação: 	2007	
Fonte/Imprenta: 	Molecular and Biochemical Parasitology, v. 154, p. 134-140, 2007.	
DOI: 	10.1016/j.molbiopara.2007.04.003	
Idioma: 	Inglês	
Conteúdo: 	Single nucleotide polymorphism (SNP) markers have been shown to be useful in genetic investigations of medically important parasites and their hosts. In this paper, we describe the prediction and validation of SNPs in ESTs of Schistosoma mansoni. We used 107,417 public sequences of S. mansoni and identified 15,614 high-quality candidate SNPs in 12,184 contigs. The presence of predicted SNPs was observed in well characterized antigens and vaccine candidates such as those coding for myosin; Sm14 and Sm23; cathepsin B and triosephosphate isomerase (TPI). Additionally, SNPs were experimentally validated for the cathepsin B. A comparative model of the S. mansoni cathepsin B was built for predicting the possible consequences of amino acid substitutions on the protein structure. An analysis of the substitutions indicated that the amino acids were mostly located on the surface of the molecule, and we found no evidence for a significant conformational change of the enzyme. However, at least one of the substitutions could result in a structural modification of an epitope	
Palavras-Chave: 	Bioinformática; Comparative modeling; Computational biology; Polimorfismo de nucleotídeo único.	
Thesagro: 	Schistosoma Mansoni.	
Thesaurus Nal: 	Bioinformatics; Cathepsin B; Models; Single nucleotide polymorphism.	
Categoria do assunto: 	X Pesquisa, Tecnologia e Engenharia	
Registro original: 	Embrapa Agricultura Digital (CNPTIA)	
Exemplares: 	CNPTIA - (11570-1) - UPC - AP - -- -  -  - 2007.00042 - DES
CNPTIA - (11570-2) - UPC - AP - DD -  -  -  - DIS