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| 190. |  | FALCÃO, R.; SIMÕES, K. C.; ARAUJO, A. C. G. Aspectos reprodutivos de acessos apomíticos facultativos de Brachiaria brizantha (Poaceae). In: CONGRESSO NACIONAL DE BOTÂNICA, 55.; REUNIÃO REGIONAL DE BOTÂNICOS DE MG, BA E ES, 26., 2004, Viçosa. Conservação, bioprospecção e biotecnologia: [resumos]. Viçosa: Sociedade Botânica do Brasil: Universidade Federal Viçosa, 2004.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 195. | | REZENDE, G. C.; GONÇALVES, J. C.; SIMÕES, J. W. Competição entre fertilizantes fosfatados em plantios de eucalipto. Silvicultura, São Paulo, v. 8, n. 28, p. 451-454, 1983. Edição dos anais do 4º Congresso Florestal Brasileiro, 1982, Belo Horizonte.| Biblioteca(s): Embrapa Florestas. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
05/12/2022 |
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Data da última atualização: |
13/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
SOUZA, E. D. de; SOUZA, J. F. da S. e; OLIVEIRA NETTO, P. M. de; CARVALHEIRA, L. de R.; BATISTA, R. I. T. P.; QUINTAO, C. C. R.; LOURO, I. D.; CAMARGO, L. S. de A. |
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Afiliação: |
ELIZA DINIZ DE SOUZA, Universidade Federal do Espírito Santo; JESSICA FERNANDA DA SILVA E SOUZA, Universidade Federal de Juiz de Fora; PEDRO MANOEL DE OLIVEIRA NETTO; LUCIANO DE REZENDE CARVALHEIRA, Universidade Federal de Minas Gerais; RIBRIO IVAN TAVARES PEREIRA BATISTA, Universidade Federal Fluminense; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; IURI DRUMOND LOURO, Universidade Federal do Espírito Santo; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL. |
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Título: |
Inhibition of Hsp90 during in vitro maturation under thermoneutral or heat shock conditions compromises the developmental competence of bovine oocytes. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Zygote, v. 30, n. 6, p. 854-862, 2022. |
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DOI: |
https://doi.org/10.1017/S0967199422000387 |
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Idioma: |
Inglês |
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Conteúdo: |
Heat shock protein 90 (Hsp90) is critical for cell homeostasis but its role on bovine oocyte maturation is not well known. We investigated the importance of Hsp90 for competence of bovine oocyte using 17-(allylamino)-17-demethoxygeldanamycin (17AAG), an inhibitor of Hsp90, during in vitro maturation (IVM). Three experiments evaluated the effect of 17AAG on developmental competence of oocytes matured in vitro under thermoneutral (38.5ºC) or heat shock (HS; 41.5ºC) temperatures. The first experiment found that the blastocyst rates were lower (P < 0.05) with 2 uM 17AAG compared with the untreated control (0 uM). The abundance of HSF1 transcripts was higher in oocytes matured with 2 ?M than with 0 and 1 uM 17AAG, whereas the abundance of HSP90AA1 and HSPA1A transcripts was lower (P < 0.05) with 1 and 2 uM than with 0 uM. The second experiment found that 2 ?M 17AAG for 12 or 24 h during IVM decreased (P < 0.05) the blastocysts rates. In the third experiment, the association of 2 uM 17AAG with HS for 12 h during IVM resulted in lower (P < 0.05) blastocysts rates than 17AAG, HS or untreated control. In conclusion, inhibition of Hsp90 during in vitro maturation compromises further embryo development; the association of Hsp90 inhibition with HS aggravates the deleterious effect of both on oocyte developmental competence. |
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Palavras-Chave: |
Choque térmico; Estresse por calor; Expressão genética; Geldanamicina. |
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Thesagro: |
Bovino; Ovulo. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02276naa a2200289 a 4500
001 2149206
005 2023-01-13
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1017/S0967199422000387$2DOI
100 1 $aSOUZA, E. D. de
245 $aInhibition of Hsp90 during in vitro maturation under thermoneutral or heat shock conditions compromises the developmental competence of bovine oocytes.$h[electronic resource]
260 $c2022
520 $aHeat shock protein 90 (Hsp90) is critical for cell homeostasis but its role on bovine oocyte maturation is not well known. We investigated the importance of Hsp90 for competence of bovine oocyte using 17-(allylamino)-17-demethoxygeldanamycin (17AAG), an inhibitor of Hsp90, during in vitro maturation (IVM). Three experiments evaluated the effect of 17AAG on developmental competence of oocytes matured in vitro under thermoneutral (38.5ºC) or heat shock (HS; 41.5ºC) temperatures. The first experiment found that the blastocyst rates were lower (P < 0.05) with 2 uM 17AAG compared with the untreated control (0 uM). The abundance of HSF1 transcripts was higher in oocytes matured with 2 ?M than with 0 and 1 uM 17AAG, whereas the abundance of HSP90AA1 and HSPA1A transcripts was lower (P < 0.05) with 1 and 2 uM than with 0 uM. The second experiment found that 2 ?M 17AAG for 12 or 24 h during IVM decreased (P < 0.05) the blastocysts rates. In the third experiment, the association of 2 uM 17AAG with HS for 12 h during IVM resulted in lower (P < 0.05) blastocysts rates than 17AAG, HS or untreated control. In conclusion, inhibition of Hsp90 during in vitro maturation compromises further embryo development; the association of Hsp90 inhibition with HS aggravates the deleterious effect of both on oocyte developmental competence.
650 $aBovino
650 $aOvulo
653 $aChoque térmico
653 $aEstresse por calor
653 $aExpressão genética
653 $aGeldanamicina
700 1 $aSOUZA, J. F. da S. e
700 1 $aOLIVEIRA NETTO, P. M. de
700 1 $aCARVALHEIRA, L. de R.
700 1 $aBATISTA, R. I. T. P.
700 1 $aQUINTAO, C. C. R.
700 1 $aLOURO, I. D.
700 1 $aCAMARGO, L. S. de A.
773 $tZygote$gv. 30, n. 6, p. 854-862, 2022.
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