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Registro Completo |
Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
14/10/2016 |
Data da última atualização: |
03/03/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ABREU, F. R. M.; DEUS, K. E. de; PEREIRA, W. J.; SILVEIRA, R. D. D.; VIANELLO, R. P.; BRONDANI, C. |
Afiliação: |
FERNANDA RAQUEL MARTINS ABREU, UNIVERSIDADE FEDERAL DE GOIÁS; KARINE EVARISTO DE DEUS, UNIVERSIDADE FEDERAL DE GOIÁS; WENDELL JACINTO PEREIRA, UNIVERSIDADE FEDERAL DE GOIÁS; RICARDO DIOGENES DIAS SILVEIRA; ROSANA PEREIRA VIANELLO, CNPAF; CLAUDIO BRONDANI, CNPAF. |
Título: |
Expression of rice genes homologous of Arabidopsis genes previously related to drought tolerance. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Australian Journal of Crop Science, v. 10, n. 9, p. 1266-1271, 2016. |
ISSN: |
1835-2707 |
Idioma: |
Inglês |
Conteúdo: |
The identification and validation of candidate genes related to traits of interest is a time consuming and expensive process and the homology among genes from different species can facilitate the identification of genes of the target species from the genomic information of a model species. This study aimed to quantify the expression of homologous rice genes previously related to drought tolerance in Arabidopsis. Five genes (CPK6, PLDa, GluR2, CesA8, and EIN2) were identified in rice by the homology of the amino acid sequence between rice and Arabidopsis. The genotypes Douradão (drought tolerant) and Primavera (drought susceptible) were subjected to a water deficit experiment, and subsequently evaluated for gene expression by qPCR for the five homologous and Lsi1 genes. The qPCR analysis clearly showed that the five homologous genes were expressed in rice, which is an indication that these genes could preserve their function in rice as a response to drought. In Douradão, of the five homologous genes, all but OsGluR2 displayed an increase in the average expression in drought treatment when compared to the control, while in Primavera, the average expression of the five genes did not differ between the control and drought treatment. In Douradão, the OsPLDa1, which showed the higher expression level in drought in relation to the control (10.82), significantly increased the gene expression in the leaf and root tissues as a response to drought, in both vegetative and reproductive stages, whereas in Primavera, this gene was suppressed in both tissues and stages under drought. Therefore, the OsPLDa1 gene was the most important in relation to drought response and is an interesting candidate for further studies in developing rice cultivars that are more tolerant to this stress. MenosThe identification and validation of candidate genes related to traits of interest is a time consuming and expensive process and the homology among genes from different species can facilitate the identification of genes of the target species from the genomic information of a model species. This study aimed to quantify the expression of homologous rice genes previously related to drought tolerance in Arabidopsis. Five genes (CPK6, PLDa, GluR2, CesA8, and EIN2) were identified in rice by the homology of the amino acid sequence between rice and Arabidopsis. The genotypes Douradão (drought tolerant) and Primavera (drought susceptible) were subjected to a water deficit experiment, and subsequently evaluated for gene expression by qPCR for the five homologous and Lsi1 genes. The qPCR analysis clearly showed that the five homologous genes were expressed in rice, which is an indication that these genes could preserve their function in rice as a response to drought. In Douradão, of the five homologous genes, all but OsGluR2 displayed an increase in the average expression in drought treatment when compared to the control, while in Primavera, the average expression of the five genes did not differ between the control and drought treatment. In Douradão, the OsPLDa1, which showed the higher expression level in drought in relation to the control (10.82), significantly increased the gene expression in the leaf and root tissues as a response to drought, in both vegetative and reproductive s... Mostrar Tudo |
Palavras-Chave: |
Gene homology. |
Thesagro: |
Acido abscisico; Arroz; Oryza sativa; Rendimento. |
Thesaurus Nal: |
Abscisic acid; Arabidopsis; Quantitative polymerase chain reaction; Rice; Yields. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/148788/1/CNPAF-2016-ajcs.pdf
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Marc: |
LEADER 02695naa a2200313 a 4500 001 2054691 005 2017-03-03 008 2016 bl uuuu u00u1 u #d 022 $a1835-2707 100 1 $aABREU, F. R. M. 245 $aExpression of rice genes homologous of Arabidopsis genes previously related to drought tolerance.$h[electronic resource] 260 $c2016 520 $aThe identification and validation of candidate genes related to traits of interest is a time consuming and expensive process and the homology among genes from different species can facilitate the identification of genes of the target species from the genomic information of a model species. This study aimed to quantify the expression of homologous rice genes previously related to drought tolerance in Arabidopsis. Five genes (CPK6, PLDa, GluR2, CesA8, and EIN2) were identified in rice by the homology of the amino acid sequence between rice and Arabidopsis. The genotypes Douradão (drought tolerant) and Primavera (drought susceptible) were subjected to a water deficit experiment, and subsequently evaluated for gene expression by qPCR for the five homologous and Lsi1 genes. The qPCR analysis clearly showed that the five homologous genes were expressed in rice, which is an indication that these genes could preserve their function in rice as a response to drought. In Douradão, of the five homologous genes, all but OsGluR2 displayed an increase in the average expression in drought treatment when compared to the control, while in Primavera, the average expression of the five genes did not differ between the control and drought treatment. In Douradão, the OsPLDa1, which showed the higher expression level in drought in relation to the control (10.82), significantly increased the gene expression in the leaf and root tissues as a response to drought, in both vegetative and reproductive stages, whereas in Primavera, this gene was suppressed in both tissues and stages under drought. Therefore, the OsPLDa1 gene was the most important in relation to drought response and is an interesting candidate for further studies in developing rice cultivars that are more tolerant to this stress. 650 $aAbscisic acid 650 $aArabidopsis 650 $aQuantitative polymerase chain reaction 650 $aRice 650 $aYields 650 $aAcido abscisico 650 $aArroz 650 $aOryza sativa 650 $aRendimento 653 $aGene homology 700 1 $aDEUS, K. E. de 700 1 $aPEREIRA, W. J. 700 1 $aSILVEIRA, R. D. D. 700 1 $aVIANELLO, R. P. 700 1 $aBRONDANI, C. 773 $tAustralian Journal of Crop Science$gv. 10, n. 9, p. 1266-1271, 2016.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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Registro Completo
Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
12/01/2010 |
Data da última atualização: |
11/10/2022 |
Tipo da produção científica: |
Documentos |
Autoria: |
LIMA, M. A. C. de; SA, I. B.; KIILL, L. H. P.; ARAUJO, J. L. P.; BORGES, R. M. E.; LIMA NETO, F. P.; SOARES, J. M.; LEAO, P. C. de S.; SILVA, P. C. G. da; CORREIA, R. C.; SILVA, A. de S.; SÁ, I. I. S.; SILVA, D. F. da. |
Afiliação: |
MARIA AUXILIADORA COELHO DE LIMA, CPATSA; IEDO BEZERRA SA, CPATSA; LUCIA HELENA PIEDADE KIILL, CPATSA; JOSE LINCOLN PINHEIRO ARAUJO, CPATSA; RITA MERCIA ESTIGARRIBIA BORGES, CPATSA; FRANCISCO PINHEIRO LIMA NETO, CPATSA; JOSÉ MONTEIRO SOARES; PATRICIA COELHO DE SOUZA LEAO, CPATSA; PEDRO CARLOS GAMA DA SILVA, CPATSA; REBERT COELHO CORREIA, CPATSA; ADERALDO DE SOUZA SILVA, CPATSA; IVAN IGHOUR SILVA SÁ; DAVI FERREIRA DA SILVA, CNPS. |
Título: |
Subsídios técnicos para a indicação geográfica de procedência do Vale do Submédio São Francisco: uva de mesa e manga. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
Petrolina: Embrapa Semiárido, 2009. |
Páginas: |
54 p. |
Descrição Física: |
il. |
Série: |
(Embrapa Semiárido. Documentos, 222). |
ISSN: |
1808-9992 |
Idioma: |
Português |
Conteúdo: |
Caracterização ambiental do Vale do Submédio São Francisco; Descrição do ecossistema e sua influência na qualidade das uvas e das mangas produzidas no Vale do Submédio São Francisco; Viticultura no Vale do Submédio São Francisco; Mangicultura no Vale do Submédio São Francisco. |
Palavras-Chave: |
Certificação; Vale do Submédio São Francisco. |
Thesagro: |
Manga; Qualidade; Uva. |
Thesaurus NAL: |
Grapes; Mangoes. |
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CPATSA-2010/42261/1/SDC222.pdf
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Marc: |
LEADER 01364nam a2200373 a 4500 001 1580188 005 2022-10-11 008 2009 bl uuuu 00u1 u #d 022 $a1808-9992 100 1 $aLIMA, M. A. C. de 245 $aSubsídios técnicos para a indicação geográfica de procedência do Vale do Submédio São Francisco$buva de mesa e manga.$h[electronic resource] 260 $aPetrolina: Embrapa Semiárido$c2009 300 $a54 p.$cil. 490 $a(Embrapa Semiárido. Documentos, 222). 520 $aCaracterização ambiental do Vale do Submédio São Francisco; Descrição do ecossistema e sua influência na qualidade das uvas e das mangas produzidas no Vale do Submédio São Francisco; Viticultura no Vale do Submédio São Francisco; Mangicultura no Vale do Submédio São Francisco. 650 $aGrapes 650 $aMangoes 650 $aManga 650 $aQualidade 650 $aUva 653 $aCertificação 653 $aVale do Submédio São Francisco 700 1 $aSA, I. B. 700 1 $aKIILL, L. H. P. 700 1 $aARAUJO, J. L. P. 700 1 $aBORGES, R. M. E. 700 1 $aLIMA NETO, F. P. 700 1 $aSOARES, J. M. 700 1 $aLEAO, P. C. de S. 700 1 $aSILVA, P. C. G. da 700 1 $aCORREIA, R. C. 700 1 $aSILVA, A. de S. 700 1 $aSÁ, I. I. S. 700 1 $aSILVA, D. F. da
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