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 | Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
25/09/2017 |
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Data da última atualização: |
12/12/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BARBIERI, S. F.; RUTHES, A. C.; PETKOWICZ, C. L. de O.; GODOY, R. C. B. de; SASSAKI, G. L.; SANTANA FILHO, A. P.; SILVEIRA, J. L. M. |
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Afiliação: |
Shayla Fernanda Barbieri, UFPR; Andrea Caroline Ruthes, Brazil Division of Glycoscience, Royal Institute of Technology; Carmen Lúcia de Oliveira Petkowicz, UFPR; ROSSANA CATIE BUENO DE GODOY, CNPF; Guilherme Lanzi Sassaki, UFPR; Arquimedes Paixão Santana Filho, UFPR; Joana Léa Meira Silveira, UFPR. |
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Título: |
Extraction, purification and structural characterization of agalactoglucomannan from the gabiroba fruit (Campomanesia xanthocarpa Berg), Myrtaceae family. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Carbohydrate Polymers, v. 174, p. 887-895, Oct. 2017. |
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DOI: |
http://dx.doi.org/10.1016/j.carbpol.2017.07.015 |
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Idioma: |
Inglês |
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Conteúdo: |
In this study, we isolated and structurally characterized, for the first time, a galactoglucomannan (GGM) from the pulp of gabiroba, a Myrtaceae family species. The HPSEC-MALLS-RI analysis showed a homoge-neous polysaccharide with molar mass of 25,340 g mol−1. The monosaccharide composition showed thatthe GGM consisted of Man:Glc:Gal in a molar ratio of 1:1:0.6. Methylation and 1D and 2D NMR analysessuggested that the main chain of the GGM consisted of -d-Glcp and -d-Manp units (1 → 4)-linked. The-d-Galp substitutions occur mainly at O-6 position of -d-Manp units. The glycosidic linkages of theGGM were evident by the presence of the characteristic signals of 4-O-substituted residues at 78.6/3.69for both -d-Glcp and -d-Manp. Furthermore, the O-6 substitutions for both -d-Glcp and -d-Manpunits were confirmed by signals at 67.1/4.00 and 3.93. The interglycosidic correlations, obtained throughthe analysis of the HMBC spectrum, further confirm the structure. |
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Palavras-Chave: |
Extraction polysaccharide; Gabiroba; Galactoglucomannan; Hemicelullose; NMR analysis. |
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Thesagro: |
Espécie nativa; Guabiroba; Hemicelulose; Polpa de fruta; Sacarídeo. |
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Thesaurus Nal: |
Campomanesia xanthocarpa; Fruit pulp; Mannans. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02109naa a2200361 a 4500
001 2076213
005 2017-12-12
008 2017 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1016/j.carbpol.2017.07.015$2DOI
100 1 $aBARBIERI, S. F.
245 $aExtraction, purification and structural characterization of agalactoglucomannan from the gabiroba fruit (Campomanesia xanthocarpa Berg), Myrtaceae family.$h[electronic resource]
260 $c2017
520 $aIn this study, we isolated and structurally characterized, for the first time, a galactoglucomannan (GGM) from the pulp of gabiroba, a Myrtaceae family species. The HPSEC-MALLS-RI analysis showed a homoge-neous polysaccharide with molar mass of 25,340 g mol−1. The monosaccharide composition showed thatthe GGM consisted of Man:Glc:Gal in a molar ratio of 1:1:0.6. Methylation and 1D and 2D NMR analysessuggested that the main chain of the GGM consisted of -d-Glcp and -d-Manp units (1 → 4)-linked. The-d-Galp substitutions occur mainly at O-6 position of -d-Manp units. The glycosidic linkages of theGGM were evident by the presence of the characteristic signals of 4-O-substituted residues at 78.6/3.69for both -d-Glcp and -d-Manp. Furthermore, the O-6 substitutions for both -d-Glcp and -d-Manpunits were confirmed by signals at 67.1/4.00 and 3.93. The interglycosidic correlations, obtained throughthe analysis of the HMBC spectrum, further confirm the structure.
650 $aCampomanesia xanthocarpa
650 $aFruit pulp
650 $aMannans
650 $aEspécie nativa
650 $aGuabiroba
650 $aHemicelulose
650 $aPolpa de fruta
650 $aSacarídeo
653 $aExtraction polysaccharide
653 $aGabiroba
653 $aGalactoglucomannan
653 $aHemicelullose
653 $aNMR analysis
700 1 $aRUTHES, A. C.
700 1 $aPETKOWICZ, C. L. de O.
700 1 $aGODOY, R. C. B. de
700 1 $aSASSAKI, G. L.
700 1 $aSANTANA FILHO, A. P.
700 1 $aSILVEIRA, J. L. M.
773 $tCarbohydrate Polymers$gv. 174, p. 887-895, Oct. 2017.
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Registro original: |
Embrapa Florestas (CNPF) |
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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
03/10/2011 |
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Data da última atualização: |
02/05/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MARRACCINI, P.; FREIRE, L. P.; ALVES, G. S. C.; VIEIRA, N. G.; VINECKY, F.; ELBELT, S.; RAMOS, H. J; MONTAGNON, C.; VIEIRA, L. G. E.; LEROY, T.; POT, D.; SILVA, V. A.; RODRIGUES, G. C.; ANDRADE, A. C. |
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Afiliação: |
CIRAD; LUCIANA PEREIRA FREIRE; GABRIEL SERGIO COSTA ALVES; NATALIA GOMES VIEIRA; FELIPE VINECKY; SÔNIA ELBERT, CENARGEN; INSTITUTO AGRONOMICO DO PARANÁ; CIRAD; INSTITUTO AGRONOMICO PARANÁ; AGAP; AGAP; EPAMIG; GUSTAVO COSTA RODRIGUES, CNPTIA; ALAN CARVALHO ANDRADE, CENARGEN. |
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Título: |
RBCS1 expression in coffee: coffea orthologs, coffea arabica homeologs, and expression variability between genotypes and under drought stress |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
BMC Plant Biology, v. 11, n. 85, 2011. |
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Idioma: |
Inglês |
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Conteúdo: |
Background: In higher plants, the inhibition of photosynthetic capacity under drought is attributable to stomatal and non-stomatal (i.e., photochemical and biochemical) effects. In particular, a disruption of photosynthetic metabolism and Rubisco regulation can be observed. Several studies reported reduced expression of the RBCS genes, which encode the Rubisco small subunit, under water stress. Results: Expression of the RBCS1 gene was analysed in the allopolyploid context of C. arabica, which originates from a natural cross between the C. canephora and C. eugenioides species. Our study revealed the existence of two homeologous RBCS1 genes in C. arabica: one carried by the C. canephora sub-genome (called CaCc) and the other carried by the C. eugenioides sub-genome (called CaCe). Using specific primer pairs for each homeolog, expression studies revealed that CaCe was expressed in C. eugenioides and C. arabica but was undetectable in C. canephora. On the other hand, CaCc was expressed in C. canephora but almost completely silenced in non-introgressed ("pure?) genotypes of C. arabica. However, enhanced CaCc expression was observed in most C. arabica cultivars with introgressed C. canephora genome. In addition, total RBCS1 expression was higher for C. arabica cultivars that had recently introgressed C. canephora genome than for ?pure? cultivars. For both species, water stress led to an important decrease in the abundance of RBCS1 transcripts. This was observed for plants grown in either greenhouse or field conditions under severe or moderate drought. However, this reduction of RBCS1 gene expression was not accompanied by a decrease in the corresponding protein in the leaves of C. canephora subjected to water withdrawal. In that case, the amount of RBCS1 was even higher under drought than under unstressed (irrigated) conditions, which suggests great stability of RBCS1 under adverse water conditions. On the other hand, for C. arabica, high nocturnal expression of RBCS1 could also explain the accumulation of the RBCS1 protein under water stress. Altogether, the results presented here suggest that the content of RBCS was not responsible for the loss of photosynthetic capacity that is commonly observed in water-stressed coffee plants. Conclusion: We showed that the CaCe homeolog was expressed in C. eugenioides and non-introgressed ("pure?) genotypes of C. arabica but that it was undetectable in C. canephora. On the other hand, the CaCc homeolog was expressed in C. canephora but highly repressed in C. arabica. Expression of the CaCc homeolog was enhanced in C. arabica cultivars that experienced recent introgression with C. canephora. For both C. canephora and C. arabica species, total RBCS1 gene expression was highly reduced with WS. Unexpectedly, the accumulation of RBCS1 protein was observed in the leaves of C. canephora under WS, possibly coming from nocturnal RBCS1 expression. These results suggest that the increase in the amount of RBCS1 protein could contribute to the antioxidative function of photorespiration in water-stressed coffee plants. MenosBackground: In higher plants, the inhibition of photosynthetic capacity under drought is attributable to stomatal and non-stomatal (i.e., photochemical and biochemical) effects. In particular, a disruption of photosynthetic metabolism and Rubisco regulation can be observed. Several studies reported reduced expression of the RBCS genes, which encode the Rubisco small subunit, under water stress. Results: Expression of the RBCS1 gene was analysed in the allopolyploid context of C. arabica, which originates from a natural cross between the C. canephora and C. eugenioides species. Our study revealed the existence of two homeologous RBCS1 genes in C. arabica: one carried by the C. canephora sub-genome (called CaCc) and the other carried by the C. eugenioides sub-genome (called CaCe). Using specific primer pairs for each homeolog, expression studies revealed that CaCe was expressed in C. eugenioides and C. arabica but was undetectable in C. canephora. On the other hand, CaCc was expressed in C. canephora but almost completely silenced in non-introgressed ("pure?) genotypes of C. arabica. However, enhanced CaCc expression was observed in most C. arabica cultivars with introgressed C. canephora genome. In addition, total RBCS1 expression was higher for C. arabica cultivars that had recently introgressed C. canephora genome than for ?pure? cultivars. For both species, water stress led to an important decrease in the abundance of RBCS1 transcripts. This was observed for plants grown i... Mostrar Tudo |
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Palavras-Chave: |
Expressão gênica; Sequência genética. |
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Thesagro: |
Café; Coffea Arábica; Coffea Canephora; Genética Molecular. |
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Thesaurus NAL: |
Gene expression; Molecular genetics. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/46090/1/1471-2229-11-85-1.pdf
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Marc: |
LEADER 04164naa a2200373 a 4500
001 1902051
005 2024-05-02
008 2011 bl uuuu u00u1 u #d
100 1 $aMARRACCINI, P.
245 $aRBCS1 expression in coffee$bcoffea orthologs, coffea arabica homeologs, and expression variability between genotypes and under drought stress$h[electronic resource]
260 $c2011
520 $aBackground: In higher plants, the inhibition of photosynthetic capacity under drought is attributable to stomatal and non-stomatal (i.e., photochemical and biochemical) effects. In particular, a disruption of photosynthetic metabolism and Rubisco regulation can be observed. Several studies reported reduced expression of the RBCS genes, which encode the Rubisco small subunit, under water stress. Results: Expression of the RBCS1 gene was analysed in the allopolyploid context of C. arabica, which originates from a natural cross between the C. canephora and C. eugenioides species. Our study revealed the existence of two homeologous RBCS1 genes in C. arabica: one carried by the C. canephora sub-genome (called CaCc) and the other carried by the C. eugenioides sub-genome (called CaCe). Using specific primer pairs for each homeolog, expression studies revealed that CaCe was expressed in C. eugenioides and C. arabica but was undetectable in C. canephora. On the other hand, CaCc was expressed in C. canephora but almost completely silenced in non-introgressed ("pure?) genotypes of C. arabica. However, enhanced CaCc expression was observed in most C. arabica cultivars with introgressed C. canephora genome. In addition, total RBCS1 expression was higher for C. arabica cultivars that had recently introgressed C. canephora genome than for ?pure? cultivars. For both species, water stress led to an important decrease in the abundance of RBCS1 transcripts. This was observed for plants grown in either greenhouse or field conditions under severe or moderate drought. However, this reduction of RBCS1 gene expression was not accompanied by a decrease in the corresponding protein in the leaves of C. canephora subjected to water withdrawal. In that case, the amount of RBCS1 was even higher under drought than under unstressed (irrigated) conditions, which suggests great stability of RBCS1 under adverse water conditions. On the other hand, for C. arabica, high nocturnal expression of RBCS1 could also explain the accumulation of the RBCS1 protein under water stress. Altogether, the results presented here suggest that the content of RBCS was not responsible for the loss of photosynthetic capacity that is commonly observed in water-stressed coffee plants. Conclusion: We showed that the CaCe homeolog was expressed in C. eugenioides and non-introgressed ("pure?) genotypes of C. arabica but that it was undetectable in C. canephora. On the other hand, the CaCc homeolog was expressed in C. canephora but highly repressed in C. arabica. Expression of the CaCc homeolog was enhanced in C. arabica cultivars that experienced recent introgression with C. canephora. For both C. canephora and C. arabica species, total RBCS1 gene expression was highly reduced with WS. Unexpectedly, the accumulation of RBCS1 protein was observed in the leaves of C. canephora under WS, possibly coming from nocturnal RBCS1 expression. These results suggest that the increase in the amount of RBCS1 protein could contribute to the antioxidative function of photorespiration in water-stressed coffee plants.
650 $aGene expression
650 $aMolecular genetics
650 $aCafé
650 $aCoffea Arábica
650 $aCoffea Canephora
650 $aGenética Molecular
653 $aExpressão gênica
653 $aSequência genética
700 1 $aFREIRE, L. P.
700 1 $aALVES, G. S. C.
700 1 $aVIEIRA, N. G.
700 1 $aVINECKY, F.
700 1 $aELBELT, S.
700 1 $aRAMOS, H. J
700 1 $aMONTAGNON, C.
700 1 $aVIEIRA, L. G. E.
700 1 $aLEROY, T.
700 1 $aPOT, D.
700 1 $aSILVA, V. A.
700 1 $aRODRIGUES, G. C.
700 1 $aANDRADE, A. C.
773 $tBMC Plant Biology$gv. 11, n. 85, 2011.
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