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| 1. |  | LEMOS, N. G.; YAMAGUCHI-SHINOZAKI, K.; NAKASHIMA, K.; MOLINA, J. C.; STOLF, R.; MORALES, A. M. R.; LUGLE, S. M.; SILVEIRA, C. A. de; MARIN, S. R. R.; BINNECK, E.; FARIAS, J. R. B.; NEUMAIER, N.; NEPOMUCENO, A. L. Introduction of genes that confer drought stress tolerance in soybean [Glycine max (L.) Merril] by biobalistic. In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. p. 256. (Embrapa Soja. Documentos, 228). Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 1 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
26/09/2017 |
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Data da última atualização: |
14/12/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
PEREIRA, W. J.; BASSINELLO, P. Z.; BRONDANI, C.; VIANELLO, R. P. |
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Afiliação: |
WENDELL JACINTO PEREIRA, UFG; PRISCILA ZACZUK BASSINELLO, CNPAF; CLAUDIO BRONDANI, CNPAF; ROSANA PEREIRA VIANELLO, CNPAF. |
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Título: |
An improved method for RNA extraction from common bean seeds and validation of reference genes for qPCR. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 17, n. 2, p. 150-158, abr./jun. 2017. |
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ISSN: |
1518-7853 |
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DOI: |
10.1590/1984-70332017v17n2a22 |
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Idioma: |
Inglês |
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Conteúdo: |
An RNA extraction method with high integrity and purity as well as the selection of adequate reference genes are prerequisites for gene expression analysis. For common bean seeds, there is no well-defined protocol that can be used in a laboratory routine for gene expression analysis. In this study, an extraction protocol for RNA from common bean seeds, which produced material with good integrity for qPCR (RIN ≥ 6.5), was optimized. In addition, 10 reference genes were evaluated under qPCR standard conditions using different tissue samples of common beans. Gene stabilities were analyzed using the delta-CT method, Bestkeeper, NormFinder and geNorm approaches. The genes β-tubulin and T197 were ranked as the most stable among the sample sets evaluated with different tissue samples, while PvAct and Pv18S were the least stable. To our knowledge, this is the first study evaluating RNA isolation methods and reference gene selection for seeds of Phaseolus vulgaris. |
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Palavras-Chave: |
Gene normalization; Grain RNA extraction; Molecular breeding. |
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Thesagro: |
Feijão; Melhoramento; Phaseolus vulgaris; RNA. |
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Thesaurus NAL: |
Beans; Gene expression. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/164321/1/CNPAF-2017-cbab.pdf
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Marc: |
LEADER 01868naa a2200289 a 4500
001 2076282
005 2017-12-14
008 2017 bl uuuu u00u1 u #d
022 $a1518-7853
024 7 $a10.1590/1984-70332017v17n2a22$2DOI
100 1 $aPEREIRA, W. J.
245 $aAn improved method for RNA extraction from common bean seeds and validation of reference genes for qPCR.$h[electronic resource]
260 $c2017
520 $aAn RNA extraction method with high integrity and purity as well as the selection of adequate reference genes are prerequisites for gene expression analysis. For common bean seeds, there is no well-defined protocol that can be used in a laboratory routine for gene expression analysis. In this study, an extraction protocol for RNA from common bean seeds, which produced material with good integrity for qPCR (RIN ≥ 6.5), was optimized. In addition, 10 reference genes were evaluated under qPCR standard conditions using different tissue samples of common beans. Gene stabilities were analyzed using the delta-CT method, Bestkeeper, NormFinder and geNorm approaches. The genes β-tubulin and T197 were ranked as the most stable among the sample sets evaluated with different tissue samples, while PvAct and Pv18S were the least stable. To our knowledge, this is the first study evaluating RNA isolation methods and reference gene selection for seeds of Phaseolus vulgaris.
650 $aBeans
650 $aGene expression
650 $aFeijão
650 $aMelhoramento
650 $aPhaseolus vulgaris
650 $aRNA
653 $aGene normalization
653 $aGrain RNA extraction
653 $aMolecular breeding
700 1 $aBASSINELLO, P. Z.
700 1 $aBRONDANI, C.
700 1 $aVIANELLO, R. P.
773 $tCrop Breeding and Applied Biotechnology, Viçosa, MG$gv. 17, n. 2, p. 150-158, abr./jun. 2017.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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