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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
06/10/2008 |
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Data da última atualização: |
22/01/2015 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
BRITO, L. G.; OLIVEIRA, M. C. de S.; ROCHA, R. B.; SILVA NETTO, F. G. da; CAVALCANTE, F. A. |
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Afiliação: |
LUCIANA GATTO BRITO, CPAF-RO; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE; RODRIGO BARROS ROCHA, CPAF-RO; Francelino Goulart da Silva Netto, Embrapa Rondônia; FRANCISCO ALOISIO CAVALCANTE, CPAF-AC. |
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Título: |
Estudo da divergência genética em linhagens brasileiras de anaplasma marginale através da utilização do gene da proteína principal de superfície MSP1α. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 15.; SEMINÁRIO DE PARASITOLOGIA VETERINÁRIA DOS PAÍSES DO MERCUSUL, 2., 2008, Curitiba. Anais... Curitiba: CBPV, 2008. |
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Idioma: |
Português |
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Conteúdo: |
Bovine anaplasmosis, caused by rickettsia Anaplasma marginale, represent one of the most important diseases ofruminants worldwide, causing significant economic losses in Brazil. This pathogen can be transmitted byRhipicephalus (Boophilus) microplus infected tick; by biting flies or by blood-contaminated fomites. The major surface proteins (msp) are involved in host-pathogen and tick-pathogen interactions and have been used as markers in A. marginale characterization and genetics studies In this study were characterized A. marginale strains obtained from naturally infected cattle from Rondônia, Acre and São Paulo State. The msp1 gene was amplified from A. marginale DNA extracted from erythrocytes by PCR in a 50 μl volume of PCR MasterMix (Promega, USA). Amplified fragments were purified and used directly for sequencing . The nucleotide and amino acid sequences of A. marginale msp1 were used for sequence alignment and genetics analysis. Multiple sequence alignment was performed using the Clustal X software. Nucleotides were coded as unordered, discrete characters with five possible character-states; A, C, G, T, or N, and gaps were coded as missing data. After a first proceeding and removal of the regions without genetic information, was carried the definitive alignment. Searches for the most parsimonious tree employed the branch and bound. The stability of the inferred topology was assessed via bootstrap analysis.. Through alignment analysis of the slighter variable region of msp1a gene, five rickettsia isolated types were identified in the A. marginale populations from São Paulo, Rondônia and Acre states. These results indicates that the strains of Acre state were less similar with São Paulo sample and not have divergence within strains obtained of samples provided MenosBovine anaplasmosis, caused by rickettsia Anaplasma marginale, represent one of the most important diseases ofruminants worldwide, causing significant economic losses in Brazil. This pathogen can be transmitted byRhipicephalus (Boophilus) microplus infected tick; by biting flies or by blood-contaminated fomites. The major surface proteins (msp) are involved in host-pathogen and tick-pathogen interactions and have been used as markers in A. marginale characterization and genetics studies In this study were characterized A. marginale strains obtained from naturally infected cattle from Rondônia, Acre and São Paulo State. The msp1 gene was amplified from A. marginale DNA extracted from erythrocytes by PCR in a 50 μl volume of PCR MasterMix (Promega, USA). Amplified fragments were purified and used directly for sequencing . The nucleotide and amino acid sequences of A. marginale msp1 were used for sequence alignment and genetics analysis. Multiple sequence alignment was performed using the Clustal X software. Nucleotides were coded as unordered, discrete characters with five possible character-states; A, C, G, T, or N, and gaps were coded as missing data. After a first proceeding and removal of the regions without genetic information, was carried the definitive alignment. Searches for the most parsimonious tree employed the branch and bound. The stability of the inferred topology was assessed via bootstrap analysis.. Through alignment analysis of the slighter variable regio... Mostrar Tudo |
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Palavras-Chave: |
Anaplasma marginele; Estudo genético advergência. |
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Thesagro: |
Gene; Proteína. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/116311/1/17860.pdf
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Marc: |
LEADER 02609nam a2200205 a 4500
001 1048520
005 2015-01-22
008 2008 bl uuuu u00u1 u #d
100 1 $aBRITO, L. G.
245 $aEstudo da divergência genética em linhagens brasileiras de anaplasma marginale através da utilização do gene da proteína principal de superfície MSP1α.
260 $aIn: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 15.; SEMINÁRIO DE PARASITOLOGIA VETERINÁRIA DOS PAÍSES DO MERCUSUL, 2., 2008, Curitiba. Anais... Curitiba: CBPV$c2008
520 $aBovine anaplasmosis, caused by rickettsia Anaplasma marginale, represent one of the most important diseases ofruminants worldwide, causing significant economic losses in Brazil. This pathogen can be transmitted byRhipicephalus (Boophilus) microplus infected tick; by biting flies or by blood-contaminated fomites. The major surface proteins (msp) are involved in host-pathogen and tick-pathogen interactions and have been used as markers in A. marginale characterization and genetics studies In this study were characterized A. marginale strains obtained from naturally infected cattle from Rondônia, Acre and São Paulo State. The msp1 gene was amplified from A. marginale DNA extracted from erythrocytes by PCR in a 50 μl volume of PCR MasterMix (Promega, USA). Amplified fragments were purified and used directly for sequencing . The nucleotide and amino acid sequences of A. marginale msp1 were used for sequence alignment and genetics analysis. Multiple sequence alignment was performed using the Clustal X software. Nucleotides were coded as unordered, discrete characters with five possible character-states; A, C, G, T, or N, and gaps were coded as missing data. After a first proceeding and removal of the regions without genetic information, was carried the definitive alignment. Searches for the most parsimonious tree employed the branch and bound. The stability of the inferred topology was assessed via bootstrap analysis.. Through alignment analysis of the slighter variable region of msp1a gene, five rickettsia isolated types were identified in the A. marginale populations from São Paulo, Rondônia and Acre states. These results indicates that the strains of Acre state were less similar with São Paulo sample and not have divergence within strains obtained of samples provided
650 $aGene
650 $aProteína
653 $aAnaplasma marginele
653 $aEstudo genético advergência
700 1 $aOLIVEIRA, M. C. de S.
700 1 $aROCHA, R. B.
700 1 $aSILVA NETTO, F. G. da
700 1 $aCAVALCANTE, F. A.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
01/11/2023 |
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Data da última atualização: |
01/11/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SILVA, J. B.; SILVA, R. L. de O.; BARROS, A. A. G. de; ABURJAILE, F. F.; MELO, N. F. de; BENKO-ISEPPON, A. M. |
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Afiliação: |
JÉSSICA BARBOZA DA SILVA, UFPE; ROBERTA LANE DE OLIVEIRA SILVA, Univasf; AGNES ANGÉLICA GUEDES DE BARROS, UFPE; FLÁVIA FIGUEIRA ABURJAILE, Fiocruz; NATONIEL FRANKLIN DE MELO, CPATSA; ANA MARIA BENKO-ISEPPON, Universidade Federal de Pernambuco. |
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Título: |
Receptor-like proteins (RLPs) in the transcriptome of Vitis spp. under inoculation of Xanthomonas citri. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
In: BRAZILIAN CONGRESS OF GENETICS, 66.; SIMPÓSIO DE CITOGENÉTICA E GENÉTICAS DE PEIXES, 19.; REUNIÃO DE GENÉTICA DE MICRORGANISMOS, 32.; CONGRESSO LATINO-AMERICANO DE GENÉTICA PARA CONSERVAÇÃO, 2., 2021, Ribeirão Preto. Abstracts. Ribeirão Preto: SBG, 2021. |
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Páginas: |
p. 590. |
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Idioma: |
Inglês |
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Conteúdo: |
Objetivou-se identificar e caracterizar transcritos que codificam proteínas RLPs em dois acessos de Vitis inoculados com Xanthomonas citri. |
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Palavras-Chave: |
Genes de resistência. |
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Thesagro: |
Cancro Bacteriano; Uva; Xanthomonas Campestris. |
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Thesaurus NAL: |
Bacterial canker; Grapes; Xanthomonas citri pv. anacardii. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1157691/1/Receptor-like-proteins-RLPs-in-the-transcriptome-of-Vitis-spp.-under-inoculation-of-Xanthomonas-citri-2021.pdf
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Marc: |
LEADER 01167nam a2200265 a 4500
001 2157691
005 2023-11-01
008 2021 bl uuuu u00u1 u #d
100 1 $aSILVA, J. B.
245 $aReceptor-like proteins (RLPs) in the transcriptome of Vitis spp. under inoculation of Xanthomonas citri.$h[electronic resource]
260 $aIn: BRAZILIAN CONGRESS OF GENETICS, 66.; SIMPÓSIO DE CITOGENÉTICA E GENÉTICAS DE PEIXES, 19.; REUNIÃO DE GENÉTICA DE MICRORGANISMOS, 32.; CONGRESSO LATINO-AMERICANO DE GENÉTICA PARA CONSERVAÇÃO, 2., 2021, Ribeirão Preto. Abstracts. Ribeirão Preto: SBG$c2021
300 $ap. 590.
520 $aObjetivou-se identificar e caracterizar transcritos que codificam proteínas RLPs em dois acessos de Vitis inoculados com Xanthomonas citri.
650 $aBacterial canker
650 $aGrapes
650 $aXanthomonas citri pv. anacardii
650 $aCancro Bacteriano
650 $aUva
650 $aXanthomonas Campestris
653 $aGenes de resistência
700 1 $aSILVA, R. L. de O.
700 1 $aBARROS, A. A. G. de
700 1 $aABURJAILE, F. F.
700 1 $aMELO, N. F. de
700 1 $aBENKO-ISEPPON, A. M.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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