Data/hora: 21/06/2024 05:45:05

Biblioteca(s): 	Embrapa Arroz e Feijão.	
Data corrente: 	29/10/2004	
Data da última atualização: 	21/12/2012	
Autoria: 	SILVA, A. L. da; ROGALSKI, M.; GUERRA, M. P.	
Título: 	Effects of different cytokinins on in vitro multiplication of Prunus 'Capdeboscq' rootstocks.	
Ano de publicação: 	2003	
Fonte/Imprenta: 	Crop Breeding and Applied Biotechnology, Londrina, v. 3, n. 2, p. 149-155, June 2003.	
Idioma: 	Inglês	
Conteúdo: 	Rootstocks for peach trees are obtained in Brazil from seeds without genetic uniformity or health guarantee. Micropropagation techniques supply genetically uniform shoots but the in vitro culture protocols must be optimized. Cytokinins are essential for breaking the apex dormancy and inducing auxiliary shoot proliferation that influence the success of in vitro multiplication. The objective of this study was to assess the effect of different cytokimins in vitro multiplication rate, and on the survival rate of apical and axyllary buds in the establishment of Prunus 'Capdeboscq' rootstocks. Apical and axyllary buds were inoculated in test tubes containing 10mL Lepoivre culture medium, supplemented with BAP (0.5 mg L-1), After two subcultures of the in vitro establishment process, 1-2 cm nodal segments were treated with kinetin, isopenterryladenine and BAP, at concentrations of 0.1; 0.5; 1.0 and 2.0 mg L-1. At the in vitro establishment phase, stem tips presented greater survival, less contamination and  no hyperhidricity. BAP gave the highest in vitro multiplication rate, with 25,9 shoots/explant at the 1.5 mg L-1. The different cytokimins did not result in differences in shoot height. The best results for the number of shoots greater than 20 mm were obtained with BAP at 1.0 mg L-1.	
Palavras-Chave: 	In vitro propagation; Peach tree; Porta-enxerto; Rootstock.	
Thesaurus Nal: 	hyperhydricity.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Arroz e Feijão (CNPAF)	
Exemplares: 	CNPAF - (21810-1) - ADD - AP - -- - 631.05 - C - 2004.00158 - DIS