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Registro Completo |
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Biblioteca(s): |
Embrapa Amapá; Embrapa Cerrados. |
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Data corrente: |
20/03/1997 |
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Data da última atualização: |
20/03/1997 |
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Autoria: |
BRAGA, H. J.; LEITE, G. B.; SILVA, L. M. da. |
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Título: |
Determinacao preliminar dos parametros a e b da equacao de Angstron para seis localidades de Santa Catarina. |
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Ano de publicação: |
1987 |
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Fonte/Imprenta: |
Florianopolis:EMPASC, 1987. |
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Páginas: |
24p |
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Série: |
(EMPASC, Documentos, 89). |
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Idioma: |
Português |
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Palavras-Chave: |
Equacao de Angstron. |
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Thesagro: |
Climatologia; Radiação Luminosa. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00543nam a2200181 a 4500
001 1560514
005 1997-03-20
008 1987 bl uuuu u0uu1 u #d
100 1 $aBRAGA, H. J.
245 $aDeterminacao preliminar dos parametros a e b da equacao de Angstron para seis localidades de Santa Catarina.
260 $aFlorianopolis:EMPASC$c1987
300 $a24p
490 $a(EMPASC, Documentos, 89).
650 $aClimatologia
650 $aRadiação Luminosa
653 $aEquacao de Angstron
700 1 $aLEITE, G. B.
700 1 $aSILVA, L. M. da
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Registro original: |
Embrapa Cerrados (CPAC) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
20/08/2021 |
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Data da última atualização: |
20/08/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SAMPAIO, L.; ANTONIO, R. P.; NASCIMENTO, M. N. do; FERNANDES JUNIOR, P. I. |
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Afiliação: |
LUCAS SAMPAIO; RAFAELA PRISCILA ANTONIO, CPATSA; MARILZA NEVES DO NASCIMENTO; PAULO IVAN FERNANDES JUNIOR, CPATSA. |
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Título: |
Molecular approaches for identification of the apomictic/sexual reproductive mechanism and genetic variability in buffel grass (Cenchrus spp.) accessions. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Genetika, v. 53, n. 2, p. 493-505, 2021. |
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DOI: |
https://doi.org/10.2298/GENSR2102493S |
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Idioma: |
Inglês |
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Conteúdo: |
Cenchrus spp. encompasses forage grasses that are especially important for drylands. Thus, information on their reproductive mechanism and genetic diversity is needed. The Active Germplasm Bank (AGB) of the Embrapa Semiárido (Tropical semiarid research center) has 115 accessions of Cenchrus spp. that were not molecularly characterized. Therefore, the objective of this work was to evaluate the genetic diversity of Cenchrus spp. accessions in the AGB, and identify their reproductive mechanisms using DNA markers. Specific SCAR markers Q8H, UGT197, and PCAB10, in addition to the SCAR marker 4HS* were used to identify genotypes with apomictic and sexual reproduction modes, respectively; the genetic diversity was characterized using the ISSR D12 (GA) 6CG and HB14 (CTC)3GC markers. The reproductive mechanism was analyzed using images for the presence or absence of the band in the expected amplicon size. For the analysis of genetic diversity, the gel images were exported to the BioNumerics Software (Applied Maths), in which polymorphism data were obtained and the dissimilarity dendrogram constructed by the UPGMA clustering method using the Dice coefficient. The markers related to the apomictic region were positive for all accessions. However, only the accessions CPATSA 102 and CPATSA 134 had positive markers using the primers for both reproduction strategies, indicating that these individuals can be facultative sexual. The cluster analysis discriminated two large groups and the accessions clustered mainly by the place of origin. It was also possible to identify duplicates within AGB. The identification and exclusion of duplicate accessions provides financial and labor savings in maintaining and managing the AGB. The two sexual accessions were selected for the breeding programs of Cenchrus spp. in Brazil. MenosCenchrus spp. encompasses forage grasses that are especially important for drylands. Thus, information on their reproductive mechanism and genetic diversity is needed. The Active Germplasm Bank (AGB) of the Embrapa Semiárido (Tropical semiarid research center) has 115 accessions of Cenchrus spp. that were not molecularly characterized. Therefore, the objective of this work was to evaluate the genetic diversity of Cenchrus spp. accessions in the AGB, and identify their reproductive mechanisms using DNA markers. Specific SCAR markers Q8H, UGT197, and PCAB10, in addition to the SCAR marker 4HS* were used to identify genotypes with apomictic and sexual reproduction modes, respectively; the genetic diversity was characterized using the ISSR D12 (GA) 6CG and HB14 (CTC)3GC markers. The reproductive mechanism was analyzed using images for the presence or absence of the band in the expected amplicon size. For the analysis of genetic diversity, the gel images were exported to the BioNumerics Software (Applied Maths), in which polymorphism data were obtained and the dissimilarity dendrogram constructed by the UPGMA clustering method using the Dice coefficient. The markers related to the apomictic region were positive for all accessions. However, only the accessions CPATSA 102 and CPATSA 134 had positive markers using the primers for both reproduction strategies, indicating that these individuals can be facultative sexual. The cluster analysis discriminated two large groups and the acce... Mostrar Tudo |
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Palavras-Chave: |
Diversidade genética; Seleção assistida por marcadores. |
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Thesagro: |
Apomixia; Capim Buffel; Germoplasma; Gramínea Forrageira; Melhoramento Genético Animal; Pastagem. |
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Thesaurus NAL: |
Apomixis; Breeding; Cenchrus; Forage grasses; Germplasm; Pastures. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02870naa a2200337 a 4500
001 2133769
005 2021-08-20
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.2298/GENSR2102493S$2DOI
100 1 $aSAMPAIO, L.
245 $aMolecular approaches for identification of the apomictic/sexual reproductive mechanism and genetic variability in buffel grass (Cenchrus spp.) accessions.$h[electronic resource]
260 $c2021
520 $aCenchrus spp. encompasses forage grasses that are especially important for drylands. Thus, information on their reproductive mechanism and genetic diversity is needed. The Active Germplasm Bank (AGB) of the Embrapa Semiárido (Tropical semiarid research center) has 115 accessions of Cenchrus spp. that were not molecularly characterized. Therefore, the objective of this work was to evaluate the genetic diversity of Cenchrus spp. accessions in the AGB, and identify their reproductive mechanisms using DNA markers. Specific SCAR markers Q8H, UGT197, and PCAB10, in addition to the SCAR marker 4HS* were used to identify genotypes with apomictic and sexual reproduction modes, respectively; the genetic diversity was characterized using the ISSR D12 (GA) 6CG and HB14 (CTC)3GC markers. The reproductive mechanism was analyzed using images for the presence or absence of the band in the expected amplicon size. For the analysis of genetic diversity, the gel images were exported to the BioNumerics Software (Applied Maths), in which polymorphism data were obtained and the dissimilarity dendrogram constructed by the UPGMA clustering method using the Dice coefficient. The markers related to the apomictic region were positive for all accessions. However, only the accessions CPATSA 102 and CPATSA 134 had positive markers using the primers for both reproduction strategies, indicating that these individuals can be facultative sexual. The cluster analysis discriminated two large groups and the accessions clustered mainly by the place of origin. It was also possible to identify duplicates within AGB. The identification and exclusion of duplicate accessions provides financial and labor savings in maintaining and managing the AGB. The two sexual accessions were selected for the breeding programs of Cenchrus spp. in Brazil.
650 $aApomixis
650 $aBreeding
650 $aCenchrus
650 $aForage grasses
650 $aGermplasm
650 $aPastures
650 $aApomixia
650 $aCapim Buffel
650 $aGermoplasma
650 $aGramínea Forrageira
650 $aMelhoramento Genético Animal
650 $aPastagem
653 $aDiversidade genética
653 $aSeleção assistida por marcadores
700 1 $aANTONIO, R. P.
700 1 $aNASCIMENTO, M. N. do
700 1 $aFERNANDES JUNIOR, P. I.
773 $tGenetika$gv. 53, n. 2, p. 493-505, 2021.
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