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Registro Completo |
Biblioteca(s): |
Embrapa Clima Temperado. |
Data corrente: |
29/11/2021 |
Data da última atualização: |
29/11/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MAIA, D. S. V.; HAUBERT, L.; WÜRFEL, S. de F. R.; KRONING, I. S.; CARDOSO, M. R. de I.; LOPES, G. V.; FIORENTINI, A. M.; SILVA, W. P. |
Afiliação: |
DARLA SILVEIRA VOLCAN MAIA; LOUISE HAUBERT; SIMONE DE FATIMA RAUBER WÜRFEL; ISABELA SCHNEID KRONING; MARISA RIBEIRO DE ITAPEMA CARDOSO; GRACIELA VÖLZ LOPES; Â NGELA MARIA FIORENTINI; WLADIMIR PADILHA DA SILVA. |
Título: |
Listeria monocytogenes in sliced cheese and ham from retail markets in southern Brazil. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
FEMS Microbiology Letters, v. 366, n. 22,p. 1-6, 2019. |
Idioma: |
Inglês |
Conteúdo: |
ABSTRACT The aims of this study were to evaluate the occurrence of Listeria monocytogenes and Salmonella spp. in sliced cheese and ham from retail markets in southern Brazil, as well as to perform molecular characterization and to assess the antimicrobial resistance profile of the isolates. Samples (n = 160) of sliced cheese and ham were collected at retail level from the city of Pelotas, Brazil. The isolation of L. monocytogenes and Salmonella spp. was performed and the isolates were confirmed by PCR, submitted to antimicrobial susceptibility testing and pulsed-field gel electrophoresis (PFGE). Listeria monocytogenes was found in 9.4% (15/160) of the samples. All L. monocytogenes isolates were positive for the prs, inlA, inlC and inlJ genes. Salmonella spp. was not isolated. Regarding the antimicrobial susceptibility, one (6.6%) L. monocytogenes isolate was resistant to streptomycin and four (26.6%) to clindamycin. Macrorestriction analysis with ApaI and AscI enzymes yielded two major PFGE groups I and II. All L. monocytogenes isolates showed virulence genes, and some of them were resistant to clinically used antimicrobials, representing a risk to public health. Moreover, PFGE patterns with high similarity were visualized in L. monocytogenes isolates at different times, demonstrating adaptability of the pathogen at retail level in the region. |
Palavras-Chave: |
Antimicrobial resistance; Foodborne pathogen; PFGE RTE foods; Serotyping; Virulence genes. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02145naa a2200265 a 4500 001 2136775 005 2021-11-29 008 2019 bl uuuu u00u1 u #d 100 1 $aMAIA, D. S. V. 245 $aListeria monocytogenes in sliced cheese and ham from retail markets in southern Brazil.$h[electronic resource] 260 $c2019 520 $aABSTRACT The aims of this study were to evaluate the occurrence of Listeria monocytogenes and Salmonella spp. in sliced cheese and ham from retail markets in southern Brazil, as well as to perform molecular characterization and to assess the antimicrobial resistance profile of the isolates. Samples (n = 160) of sliced cheese and ham were collected at retail level from the city of Pelotas, Brazil. The isolation of L. monocytogenes and Salmonella spp. was performed and the isolates were confirmed by PCR, submitted to antimicrobial susceptibility testing and pulsed-field gel electrophoresis (PFGE). Listeria monocytogenes was found in 9.4% (15/160) of the samples. All L. monocytogenes isolates were positive for the prs, inlA, inlC and inlJ genes. Salmonella spp. was not isolated. Regarding the antimicrobial susceptibility, one (6.6%) L. monocytogenes isolate was resistant to streptomycin and four (26.6%) to clindamycin. Macrorestriction analysis with ApaI and AscI enzymes yielded two major PFGE groups I and II. All L. monocytogenes isolates showed virulence genes, and some of them were resistant to clinically used antimicrobials, representing a risk to public health. Moreover, PFGE patterns with high similarity were visualized in L. monocytogenes isolates at different times, demonstrating adaptability of the pathogen at retail level in the region. 653 $aAntimicrobial resistance 653 $aFoodborne pathogen 653 $aPFGE RTE foods 653 $aSerotyping 653 $aVirulence genes 700 1 $aHAUBERT, L. 700 1 $aWÜRFEL, S. de F. R. 700 1 $aKRONING, I. S. 700 1 $aCARDOSO, M. R. de I. 700 1 $aLOPES, G. V. 700 1 $aFIORENTINI, A. M. 700 1 $aSILVA, W. P. 773 $tFEMS Microbiology Letters$gv. 366, n. 22,p. 1-6, 2019.
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85. | | PINTO, A. C. de Q.; ANDRADE, S. R. M.; AMARO, A. A.; GOMES, U. Mango industry in Brazil. In: INTERNATIONAL MANGO SYMPOSIUM, 7., 2002, Recife. Program and abstracts. Fortaleza: Embrapa Agroindustria Tropical; Planaltina: Embrapa Cerrados; Cruz das Almas: Embrapa Mandioca e Fruticultura; Teresina: Embrapa Meio Norte; Petrolina: Embrapa Semi-Arido; Recife: IPA, 2002. p. 41. (Embrapa Agroindustria Tropical. Documentos, 46).Biblioteca(s): Embrapa Cerrados. |
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86. | | PINTO, A. C. de Q.; ANDRADE, S. R. M.; AMARO, A. A.; GOMES, U. Mango industry in Brazil. In: INTERNATIONAL MANGO SYMPOSIUM, 7., 2002, Recife. Proceedings... Belgium: ISHS, 2004. p. 37-50. Editado por: PINTO, A. C. Q.; PEREIRA, M. E. C.; ALVES, R. E.Biblioteca(s): Embrapa Cerrados. |
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87. | | PINTO, A. C. de Q.; SILVA, D. J.; PINTO, P. A. da C. Mango. In: JOHNSTON, A. E. (Ed.). Tropical fruits of Brazil : fertilizing for high yield and quality. Horgen: IPI, 2007. cap. 7, p. 123-142. (IPI. Bulletin, 18).Tipo: Capítulo em Livro Técnico-Científico |
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93. | | SILVA, D. J.; PINTO, J. M.; PINTO, A. C. de Q. Riego y fertirrigación. In: AL HINAI, Y. K.; AL SAADI, A. A.; AL SAQRI, F. A.; THANI, Q. R. A. (ed.). La enciclopedia del mango. Producción y usos del mango. Muscat, Sultanato de Omán, Royal Court Affairs; Hildeshein, Alemanha, GEORG OLMS AG, 2015. v. 2. pt. 1, cap. 6, p. 243-296. Il. color. Deseño y producción de WEISS-FREIBURG GMBH,Friburgo, Alemanha; Ilustración de portada de Rosie Sanders, Devon, Reino Unido.Tipo: Capítulo em Livro Técnico-Científico |
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