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Registro Completo |
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Biblioteca(s): |
Embrapa Agroindústria Tropical. |
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Data corrente: |
20/01/2015 |
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Data da última atualização: |
02/08/2019 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
MIRANDA, F. R. de; SILVA, V. B. da; SANTOS, F. S. R. dos; SILVA, C. de F. B. da; ROSSETTI, A. G.; SOARES, I. |
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Afiliação: |
FABIO RODRIGUES DE MIRANDA, CNPAT; SILVA, V. B. da, UFC - Estudante de Agronomia; SANTOS, F. S. R. dos, UFC - Mestrando em Fitotecnia; CHRISTIANA DE FATIMA BRUCE DA SILVA, CNPAT; ADROALDO GUIMARAES ROSSETTI, CNPAT; SOARES, I., UFC - Prof. Doutor Depto. de Solos. |
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Título: |
Produtividade e eficiência de utilização da água do morangueiro em cultivo hidropônico fechado utilizando substrato de fibra de coco. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
In: INOVAGRI INTERNATIONAL MEETING, 2., 2014, Fortaleza. Anais... Campina Grande: BibliotekeVirtual, 2014. |
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Páginas: |
p. 1181-1186. |
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Idioma: |
Português |
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Palavras-Chave: |
Fibra de coco; Fragaria x ananassa; Hidroponia. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/116017/1/AAC14003.pdf
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Marc: |
LEADER 00745nam a2200205 a 4500
001 2006167
005 2019-08-02
008 2014 bl uuuu u00u1 u #d
100 1 $aMIRANDA, F. R. de
245 $aProdutividade e eficiência de utilização da água do morangueiro em cultivo hidropônico fechado utilizando substrato de fibra de coco.$h[electronic resource]
260 $aIn: INOVAGRI INTERNATIONAL MEETING, 2., 2014, Fortaleza. Anais... Campina Grande: BibliotekeVirtual$c2014
300 $ap. 1181-1186.
653 $aFibra de coco
653 $aFragaria x ananassa
653 $aHidroponia
700 1 $aSILVA, V. B. da
700 1 $aSANTOS, F. S. R. dos
700 1 $aSILVA, C. de F. B. da
700 1 $aROSSETTI, A. G.
700 1 $aSOARES, I.
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Registro original: |
Embrapa Agroindústria Tropical (CNPAT) |
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Classificação |
Cutter |
Registro |
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URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Clima Temperado; Embrapa Soja. |
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Data corrente: |
20/02/2014 |
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Data da última atualização: |
05/04/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
NAKAYAMA, T. J.; RODRIGUES, F. A.; NEUMAIER, N.; MARCELINO-GUIMARÃES, F. C.; FARIAS, J. R. B.; OLIVEIRA, M. C. N. de; BORÉM, A.; OLIVEIRA, A. C. B. de; EMYGDIO, B. M.; NEPOMUCENO, A. L. |
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Afiliação: |
UEL; CNPSo; NORMAN NEUMAIER, CNPSO; FRANCISMAR CORREA MARCELINO GUIMARÃES, CNPSO; JOSE RENATO BOUCAS FARIAS, CNPSO; MARIA CRISTINA NEVES DE OLIVEIRA, CNPSO; UFV; ANA CLAUDIA BARNECHE DE OLIVEIRA, CPACT; BEATRIZ MARTI EMYGDIO, CPACT; ALEXANDRE LIMA NEPOMUCENO, SRI. |
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Título: |
Reference genes for quantitative real-time polymerase chain reaction studies in soybean plants under hypoxic conditions. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Genetics and Molecular Research, Ribeirão Preto, v. 13, n. 1, p. 860-871, 2014. |
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DOI: |
DOI: 10.4238/2014.February.13.4 |
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Idioma: |
Inglês |
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Conteúdo: |
Quantitative real-time polymerase chain reaction (RT-qPCR) is a powerful tool used to measure gene expression. However, because of its high sensitivity, the method is strongly influenced by the quality and concentration of the template cDNA and by the amplification efficiency. Relative quantification is an effective strategy for correcting random and systematic errors by using the expression level of reference gene(s) to normalize the expression level of the genes of interest. To identify soybean reference genes for use in studies of flooding stress, we compared 5 candidate reference genes (CRGs) with the NormFinder and GeNorm programs to select the best internal control. The expression stability of the CRGs was evaluated in root tissues from soybean plants subjected to hypoxic conditions. Elongation factor 1-beta and actin-11 were identified as the most appropriate genes for RT-qPCR normalization by both the NormFinder and GeNorm analyses. The expression profiles of the genes for alcohol dehydrogenase 1, sucrose synthase 4, and ascorbate peroxidase 2 were analyzed by comparing different normalizing combinations (including no normalization) of the selected reference genes. Here, we have identified potential genes for use as references for RT-qPCR normalization in experiments with soybean roots growing in O2-depleted environments, such as flooding-stressed plants. |
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Palavras-Chave: |
Endogenous genes; Flooding; Housekeeping genes; Internal control genes. |
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Thesaurus NAL: |
gene expression. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/97888/1/nakayama.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/97827/1/Ana-Claudia.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/112937/1/Reference-genes-soybean-..hypoxic-conditions-Incluido.pdf
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Marc: |
LEADER 02358naa a2200301 a 4500
001 1980784
005 2022-04-05
008 2014 bl uuuu u00u1 u #d
024 7 $aDOI: 10.4238/2014.February.13.4$2DOI
100 1 $aNAKAYAMA, T. J.
245 $aReference genes for quantitative real-time polymerase chain reaction studies in soybean plants under hypoxic conditions.$h[electronic resource]
260 $c2014
520 $aQuantitative real-time polymerase chain reaction (RT-qPCR) is a powerful tool used to measure gene expression. However, because of its high sensitivity, the method is strongly influenced by the quality and concentration of the template cDNA and by the amplification efficiency. Relative quantification is an effective strategy for correcting random and systematic errors by using the expression level of reference gene(s) to normalize the expression level of the genes of interest. To identify soybean reference genes for use in studies of flooding stress, we compared 5 candidate reference genes (CRGs) with the NormFinder and GeNorm programs to select the best internal control. The expression stability of the CRGs was evaluated in root tissues from soybean plants subjected to hypoxic conditions. Elongation factor 1-beta and actin-11 were identified as the most appropriate genes for RT-qPCR normalization by both the NormFinder and GeNorm analyses. The expression profiles of the genes for alcohol dehydrogenase 1, sucrose synthase 4, and ascorbate peroxidase 2 were analyzed by comparing different normalizing combinations (including no normalization) of the selected reference genes. Here, we have identified potential genes for use as references for RT-qPCR normalization in experiments with soybean roots growing in O2-depleted environments, such as flooding-stressed plants.
650 $agene expression
653 $aEndogenous genes
653 $aFlooding
653 $aHousekeeping genes
653 $aInternal control genes
700 1 $aRODRIGUES, F. A.
700 1 $aNEUMAIER, N.
700 1 $aMARCELINO-GUIMARÃES, F. C.
700 1 $aFARIAS, J. R. B.
700 1 $aOLIVEIRA, M. C. N. de
700 1 $aBORÉM, A.
700 1 $aOLIVEIRA, A. C. B. de
700 1 $aEMYGDIO, B. M.
700 1 $aNEPOMUCENO, A. L.
773 $tGenetics and Molecular Research, Ribeirão Preto$gv. 13, n. 1, p. 860-871, 2014.
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Registro original: |
Embrapa Soja (CNPSO) |
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