Data/hora: 01/06/2024 05:28:20

Biblioteca(s): 	Embrapa Recursos Genéticos e Biotecnologia; Embrapa Suínos e Aves.	
Data corrente: 	23/05/2023	
Data da última atualização: 	12/06/2023	
Autoria: 	SILVA, T. C. F.; DODE, M. A. N.; BRAGA, T. F.; MARQUES, M. G.; VARGAS, L. N.; FARIA, O. A. C. de; SOUZA, A. P. de; ALBRING, D.; CAETANO, A. R.; FRANCO, M. M.	
Afiliação: 	THAINARA C. F. SILVA, Universidade Federal de Uberlândia; MARGOT ALVES NUNES DODE, Cenargen; THIAGO F. BRAGA, Centro Universitário do Cerrado; MARIANA GROKE MARQUES, CNPSA; LUNA NASCIMENTO VARGAS, Universidade Federal de Uberlândia; OTÁVIO AUGUSTO COSTA DE FARIA, Universidade de Brasília; ANDRESSA P. DE SOUZA, Universidade do Estado de Santa Catarina; DANIELA ALBRING, Universidade do Contestado; ALEXANDRE RODRIGUES CAETANO, Cenargen; MAURICIO MACHAIM FRANCO, Cenargen.	
Título: 	Cumulus-oocyte complexes from sows show differences in lipid metabolism compared to cumulus-oocyte complexes from prepubertal gilts during in vitro maturation.	
Ano de publicação: 	2023	
Fonte/Imprenta: 	Molecular Reproduction & Development, v. 90, n. 5, p. 323-335. 2023.	
DOI: 	https://doi.org/10.1002/mrd.23685	
Idioma: 	Inglês	
Conteúdo: 	Abstract: This study aimed to evaluate the effects of donor age on lipid metabolism during in
vitro maturation (IVM) of pigs cumulus?oocyte complexes (COCs). We evaluated
transcript levels of genes, the percentage of ooplasm occupied by lipid droplets (LD)
and evaluated DNA methylation in COCs from sows and prepubertal gilts. Transcript
levels of six genes (ACACA, ACSS2, FASN, FABP3, SLC27A4, PLIN2), which were
analyzed in cumulus cells (CCs), increased after 44 h of IVM in the sow group. In the
gilt group, only FASN expression increased, while NR3C1 expression decreased after
IVM. The measurement of LD in oocytes showed an accumulation of lipids in sow
oocytes during IVM, while gilt oocytes showed a decrease in LD. FABP3 and NR3C1
methylation patterns exhibited a demethylation pattern in CCs and oocytes from
gilts and sows and showed statistical differences between groups. CCs from sows
had a better capacity to change transcription levels of the major genes involved in
lipid metabolism during IVM than CCs from gilts. This difference may be involved
in accumulation of lipids, acquisition of competence, and maturation of enclosed
oocytes. Our results contribute to a better understanding of mechanisms involved in
lipid metabolism and acquisition of competence in porcine COCs.	
Palavras-Chave: 	Donor age; Embryo production; Expressão gênica; Fatty acid; Idade do doador; Pig; Produção de embriões.	
Thesagro: 	Ácido Graxo; Embrião Animal; Reprodução Animal; Suinocultura.	
Thesaurus Nal: 	Gene expression.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Suínos e Aves (CNPSA)	
Exemplares: 	CENARGEN - (39154-1) - UPC - AP - DD -  -  -  - DIS
CNPSA - (22553-1) - UPC - AP - DD -  -  -  - DIS