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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
31/01/2013 |
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Data da última atualização: |
24/04/2013 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
EIRAS, M.; SILVA, S. R.; STUCHI, E. S.; CARVALHO, S. A.; GARCÊZ, R. M. |
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Afiliação: |
MARCELO EIRAS, Centro de Pesquisa e Desenvolvimento de Sanidade Vegetal; SIMONE R. SILVA, ESALQ; EDUARDO SANCHES STUCHI, CNPMF; SÉRGIO A. CARVALHO, IAC; RENATA M. GARCÊZ, Centro de Pesquisa e Desenvolvimento de Sanidade Vegetal. |
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Título: |
Identification and characterization of viroids in Navelina ISA 315 sweet Orange. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Tropical Plant Pathology, v. 38, n. 1, p. 58-62, 2013. |
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Idioma: |
Inglês |
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Conteúdo: |
To date, seven species of viroids have been described infecting Citrus. However, only Citrus exocortis viroid (CEVd), Hop stunt viroid (HSVd) and Citrus dwarfing viroid (CDVd) have been reported in this crop in Brazil. The aim of our work was to identify and characterize viroids infecting plants of 'Navelina ISA 315' sweet orange cultivar grafted on 'Rangpur' lime from field experiments located in Bebedouro, SP, that showed gumming in the bark and wood symptoms. Biological indexing was done on the indicator host 'Clemelin 1120' tangor plants that reacted with typical gumming symptoms. Viroid infection was assessed by graft-inoculation of buds from the field trees on 'Etrog' citron plants grafted on 'Rangpur' lime, followed by RNA extraction and sPAGE analysis. RNAs were subjected to RTPCR with primers for citrus viroids. The full-length amplified products were sequenced and compared to those available in the GenBank. The trees were found to be infected with cachexia (Ca) variants of HSVd-Ca or HSVd-Ca plus CDVd. The results indicate that efforts have to be made to increase and stimulate the indexing programs, to maintain plants healthy and to develop sanitary programs focused on reducing the spread of viroids and other graft-transmissible agents. |
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Thesaurus Nal: |
viroids. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01766naa a2200181 a 4500
001 1947183
005 2013-04-24
008 2013 bl uuuu u00u1 u #d
100 1 $aEIRAS, M.
245 $aIdentification and characterization of viroids in Navelina ISA 315 sweet Orange.
260 $c2013
520 $aTo date, seven species of viroids have been described infecting Citrus. However, only Citrus exocortis viroid (CEVd), Hop stunt viroid (HSVd) and Citrus dwarfing viroid (CDVd) have been reported in this crop in Brazil. The aim of our work was to identify and characterize viroids infecting plants of 'Navelina ISA 315' sweet orange cultivar grafted on 'Rangpur' lime from field experiments located in Bebedouro, SP, that showed gumming in the bark and wood symptoms. Biological indexing was done on the indicator host 'Clemelin 1120' tangor plants that reacted with typical gumming symptoms. Viroid infection was assessed by graft-inoculation of buds from the field trees on 'Etrog' citron plants grafted on 'Rangpur' lime, followed by RNA extraction and sPAGE analysis. RNAs were subjected to RTPCR with primers for citrus viroids. The full-length amplified products were sequenced and compared to those available in the GenBank. The trees were found to be infected with cachexia (Ca) variants of HSVd-Ca or HSVd-Ca plus CDVd. The results indicate that efforts have to be made to increase and stimulate the indexing programs, to maintain plants healthy and to develop sanitary programs focused on reducing the spread of viroids and other graft-transmissible agents.
650 $aviroids
700 1 $aSILVA, S. R.
700 1 $aSTUCHI, E. S.
700 1 $aCARVALHO, S. A.
700 1 $aGARCÊZ, R. M.
773 $tTropical Plant Pathology$gv. 38, n. 1, p. 58-62, 2013.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
16/10/2017 |
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Data da última atualização: |
18/05/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
AIDAR, S. de T.; CHAVES, A. R. de M.; FERNANDES JUNIOR, P. I.; OLIVEIRA, M. de S.; COSTA NETO, B. P. da; CALSA JUNIOR, T.; MORGANTE, C. V. |
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Afiliação: |
SAULO DE TARSO AIDAR, CPATSA; AGNALDO RODRIGUES DE MELO CHAVES, CPATSA; PAULO IVAN FERNANDES JUNIOR, CPATSA; MELQUISEDEC DE S. OLIVEIRA, UFPE; BENJAMIM P. DA COSTA NETO, UPE; TERCÍLIO CALSA JUNIOR, UFPE; CAROLINA VIANNA MORGANTE, CPATSA. |
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Título: |
Vegetative desiccation tolerance of Tripogon spicatus (Poaceae) from the tropical semiarid region of northeastern Brazil. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Functional Plant Biology, v, 44, n. 11, p. 1124-1133, 2017. |
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DOI: |
10.1071/FP17066 |
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Idioma: |
Inglês |
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Conteúdo: |
The vegetative desiccation tolerance of Tripogon spicatus (Nees) Ekman was confirmed by its ability to recover the physiological functionality of intact plants previously subjected to extreme dehydration. Photosynthesis became undetectable when leaf relative water content (RWCleaf) achieved ~60%, whereas photochemical variables showed a partial decrease. Until the minimum RWCleaf of 6.41%, total chl decreased by 9%, and total carotenoids increased by 29%. Superoxide dismutase (SOD) activity decreased by 57%, on average, during dehydration, but catalase (CAT) and peroxidase (APX) activities showed no significant differences throughout the experiment. Malondialdehyde (MDA) content increased by 151%, total leaf and root amino acids decreased by 62% and 77%, respectively, whereas leaf and root proline decreased by 40% and 61%, respectively, until complete desiccation. After rehydration, leaves completely recovered turgidity and total chl contents. Carotenoids and MDA remained high, whereas SOD was 60% lower than the measured average measured before dehydration. With the exception of root amino acid contents, total amino acids and proline concentrations recovered completely. Gas exchange and photochemical variables remained substantially higher 4 days after rehydration, compared with the control. Besides increasing MDA, the overall physiological results showed that membrane functionality was preserved, leading to the vegetative desiccation tolerance of T. spicatus during the dehydration?rehydration cycle. MenosThe vegetative desiccation tolerance of Tripogon spicatus (Nees) Ekman was confirmed by its ability to recover the physiological functionality of intact plants previously subjected to extreme dehydration. Photosynthesis became undetectable when leaf relative water content (RWCleaf) achieved ~60%, whereas photochemical variables showed a partial decrease. Until the minimum RWCleaf of 6.41%, total chl decreased by 9%, and total carotenoids increased by 29%. Superoxide dismutase (SOD) activity decreased by 57%, on average, during dehydration, but catalase (CAT) and peroxidase (APX) activities showed no significant differences throughout the experiment. Malondialdehyde (MDA) content increased by 151%, total leaf and root amino acids decreased by 62% and 77%, respectively, whereas leaf and root proline decreased by 40% and 61%, respectively, until complete desiccation. After rehydration, leaves completely recovered turgidity and total chl contents. Carotenoids and MDA remained high, whereas SOD was 60% lower than the measured average measured before dehydration. With the exception of root amino acid contents, total amino acids and proline concentrations recovered completely. Gas exchange and photochemical variables remained substantially higher 4 days after rehydration, compared with the control. Besides increasing MDA, the overall physiological results showed that membrane functionality was preserved, leading to the vegetative desiccation tolerance of T. spicatus during the dehy... Mostrar Tudo |
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Palavras-Chave: |
Desidratação vegetativa; Resurrection plant; Tripogon spicatus. |
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Thesagro: |
Caatinga; Gramínea. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02343naa a2200265 a 4500
001 2077453
005 2018-05-18
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1071/FP17066$2DOI
100 1 $aAIDAR, S. de T.
245 $aVegetative desiccation tolerance of Tripogon spicatus (Poaceae) from the tropical semiarid region of northeastern Brazil.
260 $c2017
520 $aThe vegetative desiccation tolerance of Tripogon spicatus (Nees) Ekman was confirmed by its ability to recover the physiological functionality of intact plants previously subjected to extreme dehydration. Photosynthesis became undetectable when leaf relative water content (RWCleaf) achieved ~60%, whereas photochemical variables showed a partial decrease. Until the minimum RWCleaf of 6.41%, total chl decreased by 9%, and total carotenoids increased by 29%. Superoxide dismutase (SOD) activity decreased by 57%, on average, during dehydration, but catalase (CAT) and peroxidase (APX) activities showed no significant differences throughout the experiment. Malondialdehyde (MDA) content increased by 151%, total leaf and root amino acids decreased by 62% and 77%, respectively, whereas leaf and root proline decreased by 40% and 61%, respectively, until complete desiccation. After rehydration, leaves completely recovered turgidity and total chl contents. Carotenoids and MDA remained high, whereas SOD was 60% lower than the measured average measured before dehydration. With the exception of root amino acid contents, total amino acids and proline concentrations recovered completely. Gas exchange and photochemical variables remained substantially higher 4 days after rehydration, compared with the control. Besides increasing MDA, the overall physiological results showed that membrane functionality was preserved, leading to the vegetative desiccation tolerance of T. spicatus during the dehydration?rehydration cycle.
650 $aCaatinga
650 $aGramínea
653 $aDesidratação vegetativa
653 $aResurrection plant
653 $aTripogon spicatus
700 1 $aCHAVES, A. R. de M.
700 1 $aFERNANDES JUNIOR, P. I.
700 1 $aOLIVEIRA, M. de S.
700 1 $aCOSTA NETO, B. P. da
700 1 $aCALSA JUNIOR, T.
700 1 $aMORGANTE, C. V.
773 $tFunctional Plant Biology, v, 44$gn. 11, p. 1124-1133, 2017.
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