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 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
31/01/2013 |
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Data da última atualização: |
06/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, S. M. B. da; WERNECK, J. O. S.; FALCAO, R.; GOMES, A. C. M. M.; FRAGOSO, R. R.; QUEZADO, M. T.; O. NETO, O. B.; AGUIAR, J. B.; SA, M. F. G. de; BRAVO, A.; PONTES, R. G. M. S. de. |
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Afiliação: |
JOSEILDE OLIVEIRA SILVA WERNECK, CENARGEN; ROSANA FALCAO, CENARGEN; ANA CRISTINA MENESES M GOMES, CENARGEN; MARIA FATIMA GROSSI DE SA, CENARGEN; UNAN, Cuernavaca, Mexico; ROSE GOMES MONNERAT SOLON DE PONTES, CENARGEN. |
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Título: |
Characterization of novel Brazilian Bacillus thuringiensis strains active against Spodoptera frugiperda and other insect pest. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
Journal of Applied Entomology, v. 128, n. 2, p. 102-107, 2004. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Brazilian strains of Bacillus thuringiensis, namely S701, S764 and S1265 were analysed regarding their cry gene and protein contents, crystal type, and activity against larvae of the lepidopteran fall armyworm (Spodoptera frugiperda Smith), the velvet caterpillar (Anticarsia gemmatalis), the dipterans (Culex quinquefasciatus and Aedes aegypti) and the coleopteran (Tenebrio molitor). The LC50 of the strains against second instar larvae of S. frugiperda or A. gemmatalis revealed a high potency against those insect species. The spore?crystal mixtures of the isolates were analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and showed similar protein pattern as the B. thuringiensis subsp. kurstaki strain HD-1 (proteins approximately 130 and 65 kDa) for isolates S701 and S764, respectively, and only one major protein of approximately 130 kDa for isolate S1265. The polymerase chain reaction (PCR) using total DNA of the isolates and general and speci?c primers showed the presence of cry1Aa, cry1Ac, cry1Ia and cry2Ab genes in the two isolates serotyped as B. thuringiensis kurstaki (S701 and S764) and the presence of cry1D and cry2Ad in B. thuringiensis morrisoni S1265 strain. Scanning electron microscopy of strains S701 and S764, showed the presence of bipyramidal, cuboidal and round crystals, like in strain HD-1 and bipyramidal and round crystals like in strain S1265. |
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Palavras-Chave: |
Anticarsia gemmantalis; Cry genes; Crystal protein. |
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Thesagro: |
Bacillus Thuringiensis; Spodoptera Frugiperda. |
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Thesaurus Nal: |
biological control. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02387naa a2200313 a 4500
001 1947148
005 2023-03-06
008 2004 bl uuuu u00u1 u #d
100 1 $aSILVA, S. M. B. da
245 $aCharacterization of novel Brazilian Bacillus thuringiensis strains active against Spodoptera frugiperda and other insect pest.$h[electronic resource]
260 $c2004
520 $aAbstract: Brazilian strains of Bacillus thuringiensis, namely S701, S764 and S1265 were analysed regarding their cry gene and protein contents, crystal type, and activity against larvae of the lepidopteran fall armyworm (Spodoptera frugiperda Smith), the velvet caterpillar (Anticarsia gemmatalis), the dipterans (Culex quinquefasciatus and Aedes aegypti) and the coleopteran (Tenebrio molitor). The LC50 of the strains against second instar larvae of S. frugiperda or A. gemmatalis revealed a high potency against those insect species. The spore?crystal mixtures of the isolates were analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and showed similar protein pattern as the B. thuringiensis subsp. kurstaki strain HD-1 (proteins approximately 130 and 65 kDa) for isolates S701 and S764, respectively, and only one major protein of approximately 130 kDa for isolate S1265. The polymerase chain reaction (PCR) using total DNA of the isolates and general and speci?c primers showed the presence of cry1Aa, cry1Ac, cry1Ia and cry2Ab genes in the two isolates serotyped as B. thuringiensis kurstaki (S701 and S764) and the presence of cry1D and cry2Ad in B. thuringiensis morrisoni S1265 strain. Scanning electron microscopy of strains S701 and S764, showed the presence of bipyramidal, cuboidal and round crystals, like in strain HD-1 and bipyramidal and round crystals like in strain S1265.
650 $abiological control
650 $aBacillus Thuringiensis
650 $aSpodoptera Frugiperda
653 $aAnticarsia gemmantalis
653 $aCry genes
653 $aCrystal protein
700 1 $aWERNECK, J. O. S.
700 1 $aFALCAO, R.
700 1 $aGOMES, A. C. M. M.
700 1 $aFRAGOSO, R. R.
700 1 $aQUEZADO, M. T.
700 1 $aO. NETO, O. B.
700 1 $aAGUIAR, J. B.
700 1 $aSA, M. F. G. de
700 1 $aBRAVO, A.
700 1 $aPONTES, R. G. M. S. de
773 $tJournal of Applied Entomology$gv. 128, n. 2, p. 102-107, 2004.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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| Registros recuperados : 3 | |
| 2. |  | SUJII, E. R.; FONTES, E. M. G.; PIRES, C. S. S.; SILVA, S. M. B. da; AVELAR, C. S.; GARCIA, M. A. A formiga Pachycondyla obscuricornis como agente de controle biologico da cigarrinha-das-pastagens, Deois flavopicta. In: SIMPOSIO DE CONTROLE BIOLOGICO, 5.; 1996, Foz do Iguacu, PR. Anais:sessao de posters. Londrina: EMBRAPA-CNPSO, 1996. p.120.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 3. | | SILVA, S. M. B. da; WERNECK, J. O. S.; FALCAO, R.; GOMES, A. C. M. M.; FRAGOSO, R. R.; QUEZADO, M. T.; O. NETO, O. B.; AGUIAR, J. B.; SA, M. F. G. de; BRAVO, A.; PONTES, R. G. M. S. de. Characterization of novel Brazilian Bacillus thuringiensis strains active against Spodoptera frugiperda and other insect pest. Journal of Applied Entomology, v. 128, n. 2, p. 102-107, 2004.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 3 | |
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