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Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
Data corrente: |
29/12/2023 |
Data da última atualização: |
29/12/2023 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
SILVA, S. E. da; MARAFON, A. C.; MACHADO, J. C. |
Afiliação: |
SUELEN ESPINDULA DA SILVA, UFAL; ANDERSON CARLOS MARAFON, CPATC; JUAREZ CAMPOLINA MACHADO, CNPGL. |
Título: |
Cultivar de capim-elefante adaptada ao bioma Mata-atlântica com alto teor de fibras, lignina e poder calorífico para cogeração de energia térmica. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA TABULEIROS COSTEIROS, 12., 2023, Aracaju. Anais... Aracaju: Embrapa Tabuleiros Costeiros, 2023. Editores Técnicos: Viviane Talamini, Ana da Silva Ledo. |
Idioma: |
Português |
Thesagro: |
Capim Elefante; Nutrição Animal. |
Thesaurus Nal: |
Animal nutrition. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
Marc: |
LEADER 00733nam a2200157 a 4500 001 2160317 005 2023-12-29 008 2023 bl uuuu u00u1 u #d 100 1 $aSILVA, S. E. da 245 $aCultivar de capim-elefante adaptada ao bioma Mata-atlântica com alto teor de fibras, lignina e poder calorífico para cogeração de energia térmica.$h[electronic resource] 260 $aIn: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA TABULEIROS COSTEIROS, 12., 2023, Aracaju. Anais... Aracaju: Embrapa Tabuleiros Costeiros, 2023. Editores Técnicos: Viviane Talamini, Ana da Silva Ledo.$c2023 650 $aAnimal nutrition 650 $aCapim Elefante 650 $aNutrição Animal 700 1 $aMARAFON, A. C. 700 1 $aMACHADO, J. C.
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| Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com cpac.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Cerrados. |
Data corrente: |
11/12/2020 |
Data da última atualização: |
11/12/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
ARANTES, L. G.; TONELLI, G. S. S. S.; MARTINS, C. F.; BÁO, S. N. |
Afiliação: |
CARLOS FREDERICO MARTINS, CPAC. |
Título: |
Cellular Characterization and Effectsof Cryoprotectant Solutions on the Viabilityof Fibroblasts from Three Brazilian Wild Cats. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Biopreservation and Biobanking, 2020. |
Idioma: |
Português |
Conteúdo: |
Preserving genetic material in cryogenic conditions presents a viable alternative for the protection of species' gene variability. However, there is an enormous need to establish and test cryopreservation protocols that are suitable for each diverse cell type to guarantee technical success in the long run. Considering this, fibroblasts from jaguar (Panthera onca), oncilla (Leopardus tigrinus), and pampas cat (Leopardus colocolo) were subjected to cell characterization and then cryopreservation in different cryoprotectant solutions (2.5%, 10% dimethyl sulfoxide [DMSO] or CryoSOfree?). Further testing was conducted to determine each solution's performance in preserving cell viability. In culture, a growth curve to assess cellular growth potential showed that exponential proliferation lasts for about the first 50 hours of in vitro culturing, declining in pace afterward. L. colocolo and L. tigrinus presented no difference in cell viability while using 2.5% DMSO protocols. P. onca cells did not present difference on viability for both concentrations of DMSO. Protocols using CryoSOfree resulted in a decreased viability of P. onca fibroblasts. Morphological differences between fibroblasts among the species were noted under bright field microscopy and scanning electron microscopy. L. colocolo and P. onca cells are fusiform, and L. tigrinus are spherical. All cells presented cytoplasmic projections. Transmission electron microscopy revealed vacuoles and secretion granules, indicating intense cell activity after thawing. Differences found in the efficiency of cryopreservation protocols according to the type of cryoprotectant indicate that species react differently to freezing and thawing processes. This research evaluates key aspects of in vitro protocols for cryopreservation of wild animals, which need to be optimized to guarantee successful cell culturing. More suitable protocols lead to increased efficiency in establishing fibroblast cryobanks and also facilitating the use of wild cats' cells in cloning techniques, contributing directly to preserving wild fauna. MenosPreserving genetic material in cryogenic conditions presents a viable alternative for the protection of species' gene variability. However, there is an enormous need to establish and test cryopreservation protocols that are suitable for each diverse cell type to guarantee technical success in the long run. Considering this, fibroblasts from jaguar (Panthera onca), oncilla (Leopardus tigrinus), and pampas cat (Leopardus colocolo) were subjected to cell characterization and then cryopreservation in different cryoprotectant solutions (2.5%, 10% dimethyl sulfoxide [DMSO] or CryoSOfree?). Further testing was conducted to determine each solution's performance in preserving cell viability. In culture, a growth curve to assess cellular growth potential showed that exponential proliferation lasts for about the first 50 hours of in vitro culturing, declining in pace afterward. L. colocolo and L. tigrinus presented no difference in cell viability while using 2.5% DMSO protocols. P. onca cells did not present difference on viability for both concentrations of DMSO. Protocols using CryoSOfree resulted in a decreased viability of P. onca fibroblasts. Morphological differences between fibroblasts among the species were noted under bright field microscopy and scanning electron microscopy. L. colocolo and P. onca cells are fusiform, and L. tigrinus are spherical. All cells presented cytoplasmic projections. Transmission electron microscopy revealed vacuoles and secretion granules, indicating... Mostrar Tudo |
Palavras-Chave: |
Material genético; Protocolo. |
Thesagro: |
Criopreservação. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02694naa a2200193 a 4500 001 2128006 005 2020-12-11 008 2020 bl uuuu u00u1 u #d 100 1 $aARANTES, L. G. 245 $aCellular Characterization and Effectsof Cryoprotectant Solutions on the Viabilityof Fibroblasts from Three Brazilian Wild Cats.$h[electronic resource] 260 $c2020 520 $aPreserving genetic material in cryogenic conditions presents a viable alternative for the protection of species' gene variability. However, there is an enormous need to establish and test cryopreservation protocols that are suitable for each diverse cell type to guarantee technical success in the long run. Considering this, fibroblasts from jaguar (Panthera onca), oncilla (Leopardus tigrinus), and pampas cat (Leopardus colocolo) were subjected to cell characterization and then cryopreservation in different cryoprotectant solutions (2.5%, 10% dimethyl sulfoxide [DMSO] or CryoSOfree?). Further testing was conducted to determine each solution's performance in preserving cell viability. In culture, a growth curve to assess cellular growth potential showed that exponential proliferation lasts for about the first 50 hours of in vitro culturing, declining in pace afterward. L. colocolo and L. tigrinus presented no difference in cell viability while using 2.5% DMSO protocols. P. onca cells did not present difference on viability for both concentrations of DMSO. Protocols using CryoSOfree resulted in a decreased viability of P. onca fibroblasts. Morphological differences between fibroblasts among the species were noted under bright field microscopy and scanning electron microscopy. L. colocolo and P. onca cells are fusiform, and L. tigrinus are spherical. All cells presented cytoplasmic projections. Transmission electron microscopy revealed vacuoles and secretion granules, indicating intense cell activity after thawing. Differences found in the efficiency of cryopreservation protocols according to the type of cryoprotectant indicate that species react differently to freezing and thawing processes. This research evaluates key aspects of in vitro protocols for cryopreservation of wild animals, which need to be optimized to guarantee successful cell culturing. More suitable protocols lead to increased efficiency in establishing fibroblast cryobanks and also facilitating the use of wild cats' cells in cloning techniques, contributing directly to preserving wild fauna. 650 $aCriopreservação 653 $aMaterial genético 653 $aProtocolo 700 1 $aTONELLI, G. S. S. S. 700 1 $aMARTINS, C. F. 700 1 $aBÁO, S. N. 773 $tBiopreservation and Biobanking, 2020.
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