Data/hora: 21/06/2024 20:05:13

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 10/07/2018
Data da última atualização: | 18/09/2018
Autoria: | SOUZA, F. V. D.; SOUZA, E. H. de; SILVA, R. L. da.
Afiliação: | FERNANDA VIDIGAL DUARTE SOUZA, CNPMF; EVERTON HILO DE SOUZA; RONILZE LEITE DA SILVA.
Título: | Cryopreservation of pollen grains of pineapple and other bromeliads.
Ano de publicação: | 2018
Fonte/Imprenta: | In: LOYOLA-VARGAS, V.r M; Ochoa-Alejo, N. (ed.). Plant Cell Culture Protocols, Methods in Molecular Biology. New York, NY: Humana Press, 2018.
Páginas: | 279-288
Descrição Física: | v.1815. Cap. 19.
DOI: | 0.1007/978-1-4939-8594-4_19
Idioma: | Inglês
Conteúdo: | Cryopreservation of pollen grains is an efficient technique to overcome asynchronous flowering and to support actions for genetic improvement and conservation of important alleles. It can be used both by germplasm curators and plant breeders. In the case of Bromeliaceae, a family with wide diversity but also high vulnerability, the form of conservation can be crucial to prevent the increasing problem of genetic erosion. This chapter describes a method of cryopreservation of pollen grains of different Bromeliaceae species, including pineapple, after dehydration with silica and subsequent immersion in liquid nitrogen. The efficiency of this protocol has been demonstrated by the high pollen viability percentage and produc-tion of seeds after in vivo pollination with cryopreserved grains. The protocol can be used for cryopreserv-ing pollen of many species of bromeliads and is easy to perform.
Thesagro: | Bromeliaceae; Conservação; Criopreservação; Melhoramento Vegetal.
Thesaurus Nal: | Breeding and Genetic Improvement; Conservation plants; Cryopreservation.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (32427-1) - UPC - PL - DD - Publicação digital -  -  - DIS