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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
15/06/2022 |
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Data da última atualização: |
15/06/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUSA, F. G. de; MIELKE, K. C.; CALDEIRA, D. R. M.; BALDANI, V. L. D.; BALDANI, J. I.; SILVA, R. F. da; BALBINOT, E.; KLEIN, V. A. C. |
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Afiliação: |
FABIANO GAMA DE SOUSA, INSTITUTO FEDERAL, RONDONIA; KAMILA CABRAL MIELKE, UFV; DANY ROBERTA MARQUES CALDEIRA, IF RONDONIA; VERA LUCIA DIVAN BALDANI, CNPAB; JOSE IVO BALDANI, CNPAB; ROBERLAN FERREIRA DA SILVA, IF RONDONIA; ERNANDO BALBINOT, IF RONDONIA; VANDERLEY ANTÔNIO CHOROBURA KLEIN, IF RONDONIA. |
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Título: |
Genetic diversity and inoculation of plant-growth promoting diazotrophic bacteria for production of Eucalyptus urophylla seedlings |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Australian Journal of Crop Science, v. 16, n. 1, p. 35-44, 2022. |
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ISSN: |
1835-2707 |
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DOI: |
10.21475/ajcs.22.16.01.p2953 |
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Idioma: |
Inglês |
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Conteúdo: |
The study of diversity and the established relationship between plants and endophytic bacteria contribute significantly to the plant development. This study aimed to isolate, identify plant-growth promoting diazotrophic bacteria from Eucalyptus urophylla plants and evaluate the plant seedling response to inoculation with selected strains. The study was conducted from July 2017 to August 2018 set up in a greenhouse in a completely randomized experimental design with 17 treatments and 16 replicates, with 12 strains isolated from E. urophylla, four standard bacteria and one control (not inoculated with bacteria). Twelve strains were isolated from the eucalyptus rhizosphere using the N-free semisolid media and the 16 rRNA sequencing identified species closely related to Nitrospirillum amazonense, Stenotrophomonas maltophilia, Pantoea agglomerans, Herbaspirillum frisingense and Ideonella dechloratans. All strains were able to produce indol compounds and the presence of the nifH gene (nitrogen fixation) was demonstrated by PCR analysis. There was a significant effect of the strain inoculation on the height, diameter, root dry matter, root length, root area surface and volume of the seedling plant. The strains L1E, L4E - Azospirillum amazonense and L7E Stenotrophomonas maltophilia presented high values variables of height, diameter, root dry matter, root length, root area surface and volume. Many of the bacterial strains were able to promote plant growth either by root morphology changes or biomass accumulation in root and shoots. Therefore, there is a biotechnological potential of these plant-growth promoting diazotrophic bacteria strains to be applied as inoculants to improve growth of eucalyptus seedlings. This fact opens new opportunities to establish forests with economically viable and environmentally sustainable technologies. MenosThe study of diversity and the established relationship between plants and endophytic bacteria contribute significantly to the plant development. This study aimed to isolate, identify plant-growth promoting diazotrophic bacteria from Eucalyptus urophylla plants and evaluate the plant seedling response to inoculation with selected strains. The study was conducted from July 2017 to August 2018 set up in a greenhouse in a completely randomized experimental design with 17 treatments and 16 replicates, with 12 strains isolated from E. urophylla, four standard bacteria and one control (not inoculated with bacteria). Twelve strains were isolated from the eucalyptus rhizosphere using the N-free semisolid media and the 16 rRNA sequencing identified species closely related to Nitrospirillum amazonense, Stenotrophomonas maltophilia, Pantoea agglomerans, Herbaspirillum frisingense and Ideonella dechloratans. All strains were able to produce indol compounds and the presence of the nifH gene (nitrogen fixation) was demonstrated by PCR analysis. There was a significant effect of the strain inoculation on the height, diameter, root dry matter, root length, root area surface and volume of the seedling plant. The strains L1E, L4E - Azospirillum amazonense and L7E Stenotrophomonas maltophilia presented high values variables of height, diameter, root dry matter, root length, root area surface and volume. Many of the bacterial strains were able to promote plant growth either by root morphology c... Mostrar Tudo |
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Thesaurus Nal: |
Auxins; Eucalyptus; Nitrogen fixation; Plant growth-promoting rhizobacteria. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02736naa a2200277 a 4500
001 2144116
005 2022-06-15
008 2022 bl uuuu u00u1 u #d
022 $a1835-2707
024 7 $a10.21475/ajcs.22.16.01.p2953$2DOI
100 1 $aSOUSA, F. G. de
245 $aGenetic diversity and inoculation of plant-growth promoting diazotrophic bacteria for production of Eucalyptus urophylla seedlings$h[electronic resource]
260 $c2022
520 $aThe study of diversity and the established relationship between plants and endophytic bacteria contribute significantly to the plant development. This study aimed to isolate, identify plant-growth promoting diazotrophic bacteria from Eucalyptus urophylla plants and evaluate the plant seedling response to inoculation with selected strains. The study was conducted from July 2017 to August 2018 set up in a greenhouse in a completely randomized experimental design with 17 treatments and 16 replicates, with 12 strains isolated from E. urophylla, four standard bacteria and one control (not inoculated with bacteria). Twelve strains were isolated from the eucalyptus rhizosphere using the N-free semisolid media and the 16 rRNA sequencing identified species closely related to Nitrospirillum amazonense, Stenotrophomonas maltophilia, Pantoea agglomerans, Herbaspirillum frisingense and Ideonella dechloratans. All strains were able to produce indol compounds and the presence of the nifH gene (nitrogen fixation) was demonstrated by PCR analysis. There was a significant effect of the strain inoculation on the height, diameter, root dry matter, root length, root area surface and volume of the seedling plant. The strains L1E, L4E - Azospirillum amazonense and L7E Stenotrophomonas maltophilia presented high values variables of height, diameter, root dry matter, root length, root area surface and volume. Many of the bacterial strains were able to promote plant growth either by root morphology changes or biomass accumulation in root and shoots. Therefore, there is a biotechnological potential of these plant-growth promoting diazotrophic bacteria strains to be applied as inoculants to improve growth of eucalyptus seedlings. This fact opens new opportunities to establish forests with economically viable and environmentally sustainable technologies.
650 $aAuxins
650 $aEucalyptus
650 $aNitrogen fixation
650 $aPlant growth-promoting rhizobacteria
700 1 $aMIELKE, K. C.
700 1 $aCALDEIRA, D. R. M.
700 1 $aBALDANI, V. L. D.
700 1 $aBALDANI, J. I.
700 1 $aSILVA, R. F. da
700 1 $aBALBINOT, E.
700 1 $aKLEIN, V. A. C.
773 $tAustralian Journal of Crop Science$gv. 16, n. 1, p. 35-44, 2022.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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Classificação |
Cutter |
Registro |
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Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
01/12/2014 |
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Data da última atualização: |
18/10/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
DEGRANDI, T. M.; PITA, S.; PANZERA, Y.; OLIVEIRA, E. H. C. de; MARQUES, J. R. F.; FIGUEIRO, M. R.; MARQUES, L. C.; VINADÉ, L.; GUNSKI, R. J.; GARNERO, A. del V. |
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Afiliação: |
Tiago Marafiga Degrandi, Universidade Federal do Pampa; Sebastian Pita, University of the Republic, Montevideo, Uruguay.; Yanina Panzera, University of the Republic, Montevideo, Uruguay.; Edivaldo Herculano C. de Oliveira, UFPA / Institute Evandro Chagas; JOSE RIBAMAR FELIPE MARQUES, CPATU; MARIVALDO RODRIGUES FIGUEIRO, CPATU; Larissa Coêlho Marques, UFPA; Lucia Vinadé, Universidade Federal do Pampa; Ricardo José Gunski, Universidade Federal do Pampa; Analía Del Valle Garnero, Universidade Federal do Pampa. |
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Título: |
Karyotypic evolution of ribosomal sites in buffalo subspecies and their crossbreed. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Genetics and Molecular Biology, v. 37, n. 2, p. 375-380, Apr./June 2014. |
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DOI: |
http://dx.doi.org/10.1590/S1415-47572014000300009 |
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Idioma: |
Inglês |
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Conteúdo: |
Domestic buffaloes are divided into two group based on cytogenetic characteristics and habitats: the "river buffaloes" with 2n = 50 and the "swamp buffaloes", 2n = 48. Nevertheless, their hybrids are viable, fertile and identified by a 2n = 49. In order to have a better characterization of these different cytotypes of buffaloes, and considering that NOR-bearing chromosomes are involved in the rearrangements responsible for the karyotypic differences, we applied silver staining (Ag-NOR) and performed fluorescent in situ hybridization (FISH) experiments using 18S rDNA as probe. Metaphases were obtained through blood lymphocyte culture of 21 individuals, including river, swamp and hybrid cytotypes. Ag-NOR staining revealed active NORs on six chromosome pairs (3p, 4p, 6, 21, 23, 24) in the river buffaloes, whereas the swamp buffaloes presented only five NOR-bearing pairs (4p, 6, 20, 22, 23). The F1 crossbreed had 11 chromosomes with active NORs, indicating expression of both parental chromosomes. FISH analysis confirmed the numerical divergence identified with Ag-NOR. This result is explained by the loss of the NOR located on chromosome 4p in the river buffalo, which is involved in the tandem fusion with chromosome 9 in this subspecies. A comparison with the ancestral cattle karyotype suggests that the NOR found on the 3p of the river buffalo may have originated from a duplication of ribosomal genes, resulting in the formation of new NOR sites in this subspecies. |
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Palavras-Chave: |
Bubalino; Evolução cariotípica. |
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Thesagro: |
Genética. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/112670/1/a09v37n2.pdf
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Marc: |
LEADER 02330naa a2200277 a 4500
001 2001173
005 2022-10-18
008 2014 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1590/S1415-47572014000300009$2DOI
100 1 $aDEGRANDI, T. M.
245 $aKaryotypic evolution of ribosomal sites in buffalo subspecies and their crossbreed.$h[electronic resource]
260 $c2014
520 $aDomestic buffaloes are divided into two group based on cytogenetic characteristics and habitats: the "river buffaloes" with 2n = 50 and the "swamp buffaloes", 2n = 48. Nevertheless, their hybrids are viable, fertile and identified by a 2n = 49. In order to have a better characterization of these different cytotypes of buffaloes, and considering that NOR-bearing chromosomes are involved in the rearrangements responsible for the karyotypic differences, we applied silver staining (Ag-NOR) and performed fluorescent in situ hybridization (FISH) experiments using 18S rDNA as probe. Metaphases were obtained through blood lymphocyte culture of 21 individuals, including river, swamp and hybrid cytotypes. Ag-NOR staining revealed active NORs on six chromosome pairs (3p, 4p, 6, 21, 23, 24) in the river buffaloes, whereas the swamp buffaloes presented only five NOR-bearing pairs (4p, 6, 20, 22, 23). The F1 crossbreed had 11 chromosomes with active NORs, indicating expression of both parental chromosomes. FISH analysis confirmed the numerical divergence identified with Ag-NOR. This result is explained by the loss of the NOR located on chromosome 4p in the river buffalo, which is involved in the tandem fusion with chromosome 9 in this subspecies. A comparison with the ancestral cattle karyotype suggests that the NOR found on the 3p of the river buffalo may have originated from a duplication of ribosomal genes, resulting in the formation of new NOR sites in this subspecies.
650 $aGenética
653 $aBubalino
653 $aEvolução cariotípica
700 1 $aPITA, S.
700 1 $aPANZERA, Y.
700 1 $aOLIVEIRA, E. H. C. de
700 1 $aMARQUES, J. R. F.
700 1 $aFIGUEIRO, M. R.
700 1 $aMARQUES, L. C.
700 1 $aVINADÉ, L.
700 1 $aGUNSKI, R. J.
700 1 $aGARNERO, A. del V.
773 $tGenetics and Molecular Biology$gv. 37, n. 2, p. 375-380, Apr./June 2014.
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Embrapa Amazônia Oriental (CPATU) |
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