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Registro Completo |
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
11/09/1997 |
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Data da última atualização: |
10/09/2010 |
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Autoria: |
LEON, L. L; CANTO-CAVALHEIRO, M.M.; TRAJANO-MENEZES, V.; SILVA, R. A. M. S.; D'AVILA, A. M. R.; ECHEVARRIA, A.; SANTOS, L. H. S.; GOMES CARDOSO, L.; JANSEN, A. M. |
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Afiliação: |
FIOCRUZ. Departamento de Imunologia e Parasitologia (Rio de Janeiro, RJ); EMBRAPA. Centro de Pesquisa Agropecuaria do Pantanal (Corumba, MS); UFRRJ. Departamento de Quimica (Rio de Janeiro, RJ). |
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Título: |
Synthetic amidines: a possible new therapeutic choice for the treatment of "Mal de cadeiras". |
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Ano de publicação: |
1996 |
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Fonte/Imprenta: |
Memorias do Instituto Oswaldo Cruz, Rio de Janeiro, v.91, p.107, 1996. Suplemento. |
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Idioma: |
Inglês |
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Conteúdo: |
Among all species of trypanosome, Trypanosoma evansi has the widest distribution and the greatest range of mammalian host. This parasite has been reported in Africa, Asia, the MiddleEast, South and Central America and parts of Europe. T. evansi is responsible for a devastating horse disease known as "Mal de cadeiras" in the Pantanal region, in the Mato Grosso state (the disease is known as surra in the Old World). It seems that the disease was introduced in South America during the XVIth century by Spanish settlers and in the Pantanal region in 1850 (Hoare, 1965; Santos & Sereno, 1992). However, no effective drug treatment was available until 1930 and since then, Berenyl have been used, although some resistant strain hab been noticed. "Mal de cadeiras" represent a very important economical problem and we decided to assay some new compounds which had shown to be active against other trypanosomatids (Leon et al., 1995; Canto-Cavalheiro et al., 1995). These new compounds belongs to the class of amidines: N, N'-diphenyl-4-R-benzamidine, where R=H, Br,Cl, CN, NO2, CH3 and OCH3 and the drug screening was performed in serial dilution triplicates from 160ug to 5ug solubilized in DMSO. T. evansi trypomastigotes were raised in immunosupressed (cyclophosphamide, 200mg/kg) rats injected with parasite stabilates. When rat developed infection they were bled and the parasites were separated from the blood cells by anion-exchange chromatography (DE-52). To assay the amidines, trypomastigotes (4X10 6/ml) were resuspended in phosphate saline glucose (PSG) and Schneider's medium (1:1) and incubated at 25oC. The drug activity was evaluated after 24h incubation by countings in Neubauer's chamber and the remaining parasites were inoculated in immunosupressed rats, in order to determine if there was a lost of the infective capacity. Our preliminary data showed that the most active compound were the metoxy and the methyl-derivate with LDs of 16 and 14uM, respectively. MenosAmong all species of trypanosome, Trypanosoma evansi has the widest distribution and the greatest range of mammalian host. This parasite has been reported in Africa, Asia, the MiddleEast, South and Central America and parts of Europe. T. evansi is responsible for a devastating horse disease known as "Mal de cadeiras" in the Pantanal region, in the Mato Grosso state (the disease is known as surra in the Old World). It seems that the disease was introduced in South America during the XVIth century by Spanish settlers and in the Pantanal region in 1850 (Hoare, 1965; Santos & Sereno, 1992). However, no effective drug treatment was available until 1930 and since then, Berenyl have been used, although some resistant strain hab been noticed. "Mal de cadeiras" represent a very important economical problem and we decided to assay some new compounds which had shown to be active against other trypanosomatids (Leon et al., 1995; Canto-Cavalheiro et al., 1995). These new compounds belongs to the class of amidines: N, N'-diphenyl-4-R-benzamidine, where R=H, Br,Cl, CN, NO2, CH3 and OCH3 and the drug screening was performed in serial dilution triplicates from 160ug to 5ug solubilized in DMSO. T. evansi trypomastigotes were raised in immunosupressed (cyclophosphamide, 200mg/kg) rats injected with parasite stabilates. When rat developed infection they were bled and the parasites were separated from the blood cells by anion-exchange chromatography (DE-52). To assay the amidines, trypomastigote... Mostrar Tudo |
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Palavras-Chave: |
Mal de cadeiras. |
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Thesaurus Nal: |
Brazil; Pantanal; Trypanosoma evansi. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02761naa a2200265 a 4500
001 1791634
005 2010-09-10
008 1996 bl uuuu u00u1 u #d
100 1 $aLEON, L. L
245 $aSynthetic amidines$ba possible new therapeutic choice for the treatment of "Mal de cadeiras".
260 $c1996
520 $aAmong all species of trypanosome, Trypanosoma evansi has the widest distribution and the greatest range of mammalian host. This parasite has been reported in Africa, Asia, the MiddleEast, South and Central America and parts of Europe. T. evansi is responsible for a devastating horse disease known as "Mal de cadeiras" in the Pantanal region, in the Mato Grosso state (the disease is known as surra in the Old World). It seems that the disease was introduced in South America during the XVIth century by Spanish settlers and in the Pantanal region in 1850 (Hoare, 1965; Santos & Sereno, 1992). However, no effective drug treatment was available until 1930 and since then, Berenyl have been used, although some resistant strain hab been noticed. "Mal de cadeiras" represent a very important economical problem and we decided to assay some new compounds which had shown to be active against other trypanosomatids (Leon et al., 1995; Canto-Cavalheiro et al., 1995). These new compounds belongs to the class of amidines: N, N'-diphenyl-4-R-benzamidine, where R=H, Br,Cl, CN, NO2, CH3 and OCH3 and the drug screening was performed in serial dilution triplicates from 160ug to 5ug solubilized in DMSO. T. evansi trypomastigotes were raised in immunosupressed (cyclophosphamide, 200mg/kg) rats injected with parasite stabilates. When rat developed infection they were bled and the parasites were separated from the blood cells by anion-exchange chromatography (DE-52). To assay the amidines, trypomastigotes (4X10 6/ml) were resuspended in phosphate saline glucose (PSG) and Schneider's medium (1:1) and incubated at 25oC. The drug activity was evaluated after 24h incubation by countings in Neubauer's chamber and the remaining parasites were inoculated in immunosupressed rats, in order to determine if there was a lost of the infective capacity. Our preliminary data showed that the most active compound were the metoxy and the methyl-derivate with LDs of 16 and 14uM, respectively.
650 $aBrazil
650 $aPantanal
650 $aTrypanosoma evansi
653 $aMal de cadeiras
700 1 $aCANTO-CAVALHEIRO, M.M.
700 1 $aTRAJANO-MENEZES, V.
700 1 $aSILVA, R. A. M. S.
700 1 $aD'AVILA, A. M. R.
700 1 $aECHEVARRIA, A.
700 1 $aSANTOS, L. H. S.
700 1 $aGOMES CARDOSO, L.
700 1 $aJANSEN, A. M.
773 $tMemorias do Instituto Oswaldo Cruz, Rio de Janeiro$gv.91, p.107, 1996. Suplemento.
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Registro original: |
Embrapa Pantanal (CPAP) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com cpac.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
01/11/1994 |
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Data da última atualização: |
01/11/1994 |
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Autoria: |
SAUERESSIG, T. M.; ANDRADE, R. P. de. |
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Título: |
Comportamento do carrapato de bovinos em pastagems cultivadas e nativas na regiao dos cerrados - DF. |
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Ano de publicação: |
1994 |
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Fonte/Imprenta: |
In: EMBRAPA. Centro de Pesquisa Agropecuaria dos Cerrados (Planaltina, DF). Relatorio tecnico anual do Centro de Pesquisa Agropecuaria dos Cerrados 1987/1990. Planaltina, 1994. |
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Páginas: |
p.313-321. |
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Idioma: |
Português |
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Palavras-Chave: |
Cattle tick; Natural pastures. |
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Thesagro: |
Boophilus Microplus; Bovino; Carrapato; Cerrado; Pastagem Cultivada; Pastagem Nativa. |
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Thesaurus NAL: |
sown pastures. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00815naa a2200241 a 4500
001 1549603
005 1994-11-01
008 1994 bl uuuu u00u1 u #d
100 1 $aSAUERESSIG, T. M.
245 $aComportamento do carrapato de bovinos em pastagems cultivadas e nativas na regiao dos cerrados - DF.
260 $c1994
300 $ap.313-321.
650 $asown pastures
650 $aBoophilus Microplus
650 $aBovino
650 $aCarrapato
650 $aCerrado
650 $aPastagem Cultivada
650 $aPastagem Nativa
653 $aCattle tick
653 $aNatural pastures
700 1 $aANDRADE, R. P. de
773 $tIn: EMBRAPA. Centro de Pesquisa Agropecuaria dos Cerrados (Planaltina, DF). Relatorio tecnico anual do Centro de Pesquisa Agropecuaria dos Cerrados 1987/1990. Planaltina, 1994.
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Embrapa Cerrados (CPAC) |
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