Data/hora: 21/06/2024 18:26:53

Biblioteca(s): | Embrapa Milho e Sorgo.
Data corrente: | 02/12/2013
Data da última atualização: | 17/05/2017
Autoria: | SILVA, R. B. da; VALICENTE, F. H.
Afiliação: | ROSANE BEZERRA DA SILVA; FERNANDO HERCOS VALICENTE, CNPMS.
Título: | Molecular characterization of Bacillus thuringiensis using rep-PCR.
Ano de publicação: | 2013
Fonte/Imprenta: | SpringerPlus, v. 2, p. 641-652, nov. 2013.
DOI: | 10.1186/2193-1801-2-641
Idioma: | Inglês
Conteúdo: | The genetic divergence of 65 strains of Bacillus thuringiensis (Bt) was determined using Rep-PCR. Based on the repetitive sequences the BOX primer was the most informative with 26 fragments, followed by ERIC (19) and REP (10), generating a total of 55 fragments. The dendogram shows that ten groups were formed when 45% was the average distance of the population: group 1 with 41,5% of the isolates, 33,8% of the isolates were distributed in other groups and 24,6% did not formed distinct group. 53,2% of the isolates from Embrapa are in the group 1, and 29,8% of the isolates are distributed in other groups. Bt strains from USDA and Institute Pasteur showed more variability.
Palavras-Chave: | Divergência genética; Sequência repetitiva.
Thesagro: | Bacillus Thuringiensis.
Thesaurus Nal: | Repetitive sequences.
Categoria do assunto: | --
Registro original: | Embrapa Milho e Sorgo (CNPMS)
Exemplares: | CNPMS - (25465-1) - UPC - AP - DD -  -  -  - DIS