|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
29/12/2019 |
Data da última atualização: |
06/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
WELLER, M. M. D. C. A.; FONSECA, I.; SBARDELLA, A. P.; PINTO, I. S. B.; VICCINI, L. F.; BRANDAO, H. de M.; GERN, J. C.; CARVALHO, W. A.; GUIMARÃES, A. S.; BRITO, M. A. V. P.; MUNARI, D. P.; SILVA, M. V. G. B.; MARTINS, M. F. |
Afiliação: |
HUMBERTO DE MELLO BRANDAO, CNPGL; JULIANA CARINE GERN, CNPGL; WANESSA ARAUJO CARVALHO, CNPGL; ALESSANDRO DE SA GUIMARAES, CNPGL; MARCOS VINICIUS GUALBERTO B SILVA, CNPGL; MARTA FONSECA MARTINS, CNPGL. |
Título: |
Isolated perfused udder model fortranscriptome analysis in response to Streptococcus agalactiae. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Journal of Dairy Research, v. 86, n. 3, p. 307-314, 2019. |
DOI: |
https://doi.org/10.1017/s0022029919000451 |
Idioma: |
Inglês |
Conteúdo: |
This study aimed to evaluate the transcriptional changes occurring in isolated perfused mammary alveolar tissue in response to inoculation with S. agalactiae and to identify the most affected biological functions and pathways after 3 h. Four udders taken at slaughter from cows with healthy mammary gland were perfused ex situ with warmed and gassed Tyrode's solution. Mammary alveolar tissue samples were taken from the left fore and rear quarters (IQ-inoculated quarters) before inoculation (hour 0) and at 3 h post inoculation (hpi) and at the same times from control right fore and rear quarters (not inoculated: NIQ). A total of 1756 differentially expressed genes (DEGs) were identified between IQ and NIQ at 3 hpi using edgeR package. Within this set of DEGs, 952 were up regulated and mainly involved with innate immune response and inflammatory response, e.g., CD14, CCL5, TLR2, IL-8, SAA3, as well as in transcriptional regulation such as FOS, STAT3 and NFKBIA. Genes down-regulated (804) included those involved with lipid synthesis e.g., APOC2, SCD, FABP3 and FABP4. The most affected pathways were chemokine signaling, Wnt signaling and complement and coagulation cascades, which likely reflects the early stage response of mammary tissue to S. agalactiae infection. No significant gene expression changes were detected by RNA-Seq in the others contrasts. Real time-PCR confirmed the increase in mRNA abundance of immune-related genes: TLR2, TLR4, IL-1β, and IL-10 at 3 hpi between IQ and NIQ. The expression profiles of Casp1 and Bax for any contrasts were unaffected whereas Bcl2 was increased in IQ, which suggests no induction of apoptosis during the first hours after infection. Results provided novel information regarding the early functional pathways and gene network that orchestrate innate immune responses to S. agalactiae infection. This knowledge could contribute to new strategies to enhance resistance to this disease, such as genomic selection. MenosThis study aimed to evaluate the transcriptional changes occurring in isolated perfused mammary alveolar tissue in response to inoculation with S. agalactiae and to identify the most affected biological functions and pathways after 3 h. Four udders taken at slaughter from cows with healthy mammary gland were perfused ex situ with warmed and gassed Tyrode's solution. Mammary alveolar tissue samples were taken from the left fore and rear quarters (IQ-inoculated quarters) before inoculation (hour 0) and at 3 h post inoculation (hpi) and at the same times from control right fore and rear quarters (not inoculated: NIQ). A total of 1756 differentially expressed genes (DEGs) were identified between IQ and NIQ at 3 hpi using edgeR package. Within this set of DEGs, 952 were up regulated and mainly involved with innate immune response and inflammatory response, e.g., CD14, CCL5, TLR2, IL-8, SAA3, as well as in transcriptional regulation such as FOS, STAT3 and NFKBIA. Genes down-regulated (804) included those involved with lipid synthesis e.g., APOC2, SCD, FABP3 and FABP4. The most affected pathways were chemokine signaling, Wnt signaling and complement and coagulation cascades, which likely reflects the early stage response of mammary tissue to S. agalactiae infection. No significant gene expression changes were detected by RNA-Seq in the others contrasts. Real time-PCR confirmed the increase in mRNA abundance of immune-related genes: TLR2, TLR4, IL-1β, and IL-10 at 3 hpi between IQ a... Mostrar Tudo |
Palavras-Chave: |
Mammary gland perfused; RNA-Seq. |
Thesaurus Nal: |
Dairy cattle; Immune response; Mastitis. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02978naa a2200337 a 4500 001 2117822 005 2024-02-06 008 2019 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1017/s0022029919000451$2DOI 100 1 $aWELLER, M. M. D. C. A. 245 $aIsolated perfused udder model fortranscriptome analysis in response to Streptococcus agalactiae.$h[electronic resource] 260 $c2019 520 $aThis study aimed to evaluate the transcriptional changes occurring in isolated perfused mammary alveolar tissue in response to inoculation with S. agalactiae and to identify the most affected biological functions and pathways after 3 h. Four udders taken at slaughter from cows with healthy mammary gland were perfused ex situ with warmed and gassed Tyrode's solution. Mammary alveolar tissue samples were taken from the left fore and rear quarters (IQ-inoculated quarters) before inoculation (hour 0) and at 3 h post inoculation (hpi) and at the same times from control right fore and rear quarters (not inoculated: NIQ). A total of 1756 differentially expressed genes (DEGs) were identified between IQ and NIQ at 3 hpi using edgeR package. Within this set of DEGs, 952 were up regulated and mainly involved with innate immune response and inflammatory response, e.g., CD14, CCL5, TLR2, IL-8, SAA3, as well as in transcriptional regulation such as FOS, STAT3 and NFKBIA. Genes down-regulated (804) included those involved with lipid synthesis e.g., APOC2, SCD, FABP3 and FABP4. The most affected pathways were chemokine signaling, Wnt signaling and complement and coagulation cascades, which likely reflects the early stage response of mammary tissue to S. agalactiae infection. No significant gene expression changes were detected by RNA-Seq in the others contrasts. Real time-PCR confirmed the increase in mRNA abundance of immune-related genes: TLR2, TLR4, IL-1β, and IL-10 at 3 hpi between IQ and NIQ. The expression profiles of Casp1 and Bax for any contrasts were unaffected whereas Bcl2 was increased in IQ, which suggests no induction of apoptosis during the first hours after infection. Results provided novel information regarding the early functional pathways and gene network that orchestrate innate immune responses to S. agalactiae infection. This knowledge could contribute to new strategies to enhance resistance to this disease, such as genomic selection. 650 $aDairy cattle 650 $aImmune response 650 $aMastitis 653 $aMammary gland perfused 653 $aRNA-Seq 700 1 $aFONSECA, I. 700 1 $aSBARDELLA, A. P. 700 1 $aPINTO, I. S. B. 700 1 $aVICCINI, L. F. 700 1 $aBRANDAO, H. de M. 700 1 $aGERN, J. C. 700 1 $aCARVALHO, W. A. 700 1 $aGUIMARÃES, A. S. 700 1 $aBRITO, M. A. V. P. 700 1 $aMUNARI, D. P. 700 1 $aSILVA, M. V. G. B. 700 1 $aMARTINS, M. F. 773 $tJournal of Dairy Research$gv. 86, n. 3, p. 307-314, 2019.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Gado de Leite (CNPGL) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Pantanal. Para informações adicionais entre em contato com cpap.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Agrobiologia; Embrapa Pantanal. |
Data corrente: |
13/11/2017 |
Data da última atualização: |
21/11/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 3 |
Autoria: |
SOARES, M. T. S.; GAIAD, S.; RESENDE, A. S. de; MENEZES, G. I. de; FERNANDES, F. A.; FERNANDES, A. H. B. M. |
Afiliação: |
MARCIA TOFFANI SIMAO SOARES, CNPF; SERGIO GAIAD, CNPF; ALEXANDER SILVA DE RESENDE, CNPAB; Gustavo Ibarreche de Menezes, Guará Serviços Ambientais e Produção Vegetal; FERNANDO ANTONIO FERNANDES, CPAP; ANA HELENA B MAROZZI FERNANDES, CPAP. |
Título: |
Qualidade de mudas de espécies arbóreas procedentes do Bioma Pantanal e inoculadas com fungos micorrízicos. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Pesquisa Florestal Brasileira, Colombo, v. 37, n. 91, p. 311-322, jun./set. 2017. |
DOI: |
10.4336/2017.pfb.37.91.1424 |
Idioma: |
Português |
Conteúdo: |
O objetivo do presente trabalho foi comparar a infecção micorrízica e indicadores de qualidade das mudas de Calophyllum brasiliense e Anandenanthera colubrina var. cebil procedentes do Bioma Pantanal, desenvolvidas em substrato não fumigado e sob inoculação combinada com Glomus clarum (Gc) + Gigaspora margarita (Gm). Mudas das duas espécies produzidas em condições de viveiro foram aleatoriamente selecionadas e avaliadas quanto a parâmetros de qualidade morfológica, percentual, intensidade e morfologia da colonização micorrízica de raízes finas. Concluiu-se que, sob as condições de estudo, a inoculação com Gc + Gm promove mudanças no percentual, na intensidade (A. colubrina var. cebil) ou na morfologia (C. brasiliense) da infecção radicular, quando comparadas com mudas não-inoculadas. Incremento em parâmetros de qualidade das mudas foi observado em C. brasiliense sob inoculação combinada (Gc + Gm), apontando o potencial de uso de fungos micorrízicos na produção desta espécie. |
Palavras-Chave: |
Anandenanthera colubrina; Espécie florestal; Guanandi; Inoculation; Mixorriza. |
Thesagro: |
Angico vermelho; Árvore Florestal; Espécie nativa; Inoculação; Muda; Simbiose. |
Thesaurus NAL: |
Calophyllum brasiliense; Forest trees; seedling production; symbiosis. |
Categoria do assunto: |
-- K Ciência Florestal e Produtos de Origem Vegetal |
Marc: |
LEADER 02134naa a2200373 a 4500 001 2080364 005 2017-11-21 008 2017 bl --- 0-- u #d 024 7 $a10.4336/2017.pfb.37.91.1424$2DOI 100 1 $aSOARES, M. T. S. 245 $aQualidade de mudas de espécies arbóreas procedentes do Bioma Pantanal e inoculadas com fungos micorrízicos.$h[electronic resource] 260 $c2017 520 $aO objetivo do presente trabalho foi comparar a infecção micorrízica e indicadores de qualidade das mudas de Calophyllum brasiliense e Anandenanthera colubrina var. cebil procedentes do Bioma Pantanal, desenvolvidas em substrato não fumigado e sob inoculação combinada com Glomus clarum (Gc) + Gigaspora margarita (Gm). Mudas das duas espécies produzidas em condições de viveiro foram aleatoriamente selecionadas e avaliadas quanto a parâmetros de qualidade morfológica, percentual, intensidade e morfologia da colonização micorrízica de raízes finas. Concluiu-se que, sob as condições de estudo, a inoculação com Gc + Gm promove mudanças no percentual, na intensidade (A. colubrina var. cebil) ou na morfologia (C. brasiliense) da infecção radicular, quando comparadas com mudas não-inoculadas. Incremento em parâmetros de qualidade das mudas foi observado em C. brasiliense sob inoculação combinada (Gc + Gm), apontando o potencial de uso de fungos micorrízicos na produção desta espécie. 650 $aCalophyllum brasiliense 650 $aForest trees 650 $aseedling production 650 $asymbiosis 650 $aAngico vermelho 650 $aÁrvore Florestal 650 $aEspécie nativa 650 $aInoculação 650 $aMuda 650 $aSimbiose 653 $aAnandenanthera colubrina 653 $aEspécie florestal 653 $aGuanandi 653 $aInoculation 653 $aMixorriza 700 1 $aGAIAD, S. 700 1 $aRESENDE, A. S. de 700 1 $aMENEZES, G. I. de 700 1 $aFERNANDES, F. A. 700 1 $aFERNANDES, A. H. B. M. 773 $tPesquisa Florestal Brasileira, Colombo$gv. 37, n. 91, p. 311-322, jun./set. 2017.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Pantanal (CPAP) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|