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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte; Embrapa Gado de Leite. |
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Data corrente: |
30/12/2019 |
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Data da última atualização: |
30/12/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BACANELLI, G.; OLARTE, L. C.; SILVA, M. R.; RODRIGUES, R. A.; CARNEIRO, P. A. M.; KANEENE, J. B.; PASQUATTI, T. N.; TAKATANI, H.; ZUMÁRRAGA, M. J.; ETGES, R. N.; ARAUJO, F. R.; VERBISCK, N. V. |
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Afiliação: |
GISELE BACANELLI, Universidade Federal de Mato Grosso do Sul - UFMS/Programa de Pós-Graduação em Biotecnologia e Biodiversidade da Região Centro-Oeste; LARISSA C. OLARTE, Universidade Federal de Mato Grosso do Sul - UFMS/Programa de Pós-Graduação Multicêntrica em Bioquímica e Biologia Molecular; MARCIO ROBERTO SILVA, CNPGL; RUDIELLE A. RODRIGUES, Universidade Federal de Mato Grosso do Sul - UFMS/Faculdade de Medicina Veterinária/Programa de Pós-Graduação em Ciências Veterinárias; PAULO A. M. CARNEIRO, Michigan State University/Center for Comparative Epidemiology; JOHN B. KANEENE, Michigan State University/Center for Comparative Epidemiology; TAYNARA N. PASQUATTI, Universidade Católica Dom Bosco - UCDB; HARUO TAKATANI, Agência de Defesa Agrícola do Amazonas; MARTIN J. ZUMÁRRAGA, Institute of Biotechnology, CICVyA/INTA; RODRIGO N. ETGES, Secretário de Agricultura, Pecuária e Irrigação de Porto Alegre; FLABIO RIBEIRO DE ARAUJO, CNPGC; NEWTON VALERIO VERBISCK, CNPGC. |
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Título: |
Matrix Assisted Laser Desorption Ionization-Time-of-Flight mass spectrometry identification of Mycobacterium bovis in Bovinae. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Journal the Veterinary Medical Science, v. 81, n. 10, p.1400?1408, 2019. |
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Idioma: |
Inglês |
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Conteúdo: |
In this study, Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDITOF) mass spectrometry was used to identify Mycobacterium bovis from cattle and buffalo tissue isolates from the North and South regions of Brazil, grown in solid medium and previously identified by Polymerase Chain Reaction (PCR) based on Region of Difference 4 (RD4), sequencing and spoligotyping. For this purpose, the protein extraction protocol and the mass spectra reference database were optimized for the identification of 80 clinical isolates of mycobacteria. As a result of this optimization, it was possible to identify and differentiate M. bovis from other members of the Mycobacterium tuberculosis complex with 100% specificity, 90.91% sensitivity and 91.25% reliability. MALDI-TOF MS methodology described herein provides successful identification of M. bovis within bovine/bubaline clinical samples, demonstrating its usefulness for bovine tuberculosis diagnosis in the future. |
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Palavras-Chave: |
Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDI-TOF) mass spectrometry. |
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Thesaurus Nal: |
Bovine tuberculosis; Mycobacterium bovis BCG; Mycobacterium tuberculosis complex. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/207929/1/Matrix-Assisted-Laser-Desorption-Ionization.pdf
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Marc: |
LEADER 01994naa a2200301 a 4500
001 2117863
005 2019-12-30
008 2019 bl uuuu u00u1 u #d
100 1 $aBACANELLI, G.
245 $aMatrix Assisted Laser Desorption Ionization-Time-of-Flight mass spectrometry identification of Mycobacterium bovis in Bovinae.$h[electronic resource]
260 $c2019
520 $aIn this study, Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDITOF) mass spectrometry was used to identify Mycobacterium bovis from cattle and buffalo tissue isolates from the North and South regions of Brazil, grown in solid medium and previously identified by Polymerase Chain Reaction (PCR) based on Region of Difference 4 (RD4), sequencing and spoligotyping. For this purpose, the protein extraction protocol and the mass spectra reference database were optimized for the identification of 80 clinical isolates of mycobacteria. As a result of this optimization, it was possible to identify and differentiate M. bovis from other members of the Mycobacterium tuberculosis complex with 100% specificity, 90.91% sensitivity and 91.25% reliability. MALDI-TOF MS methodology described herein provides successful identification of M. bovis within bovine/bubaline clinical samples, demonstrating its usefulness for bovine tuberculosis diagnosis in the future.
650 $aBovine tuberculosis
650 $aMycobacterium bovis BCG
650 $aMycobacterium tuberculosis complex
653 $aMatrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDI-TOF) mass spectrometry
700 1 $aOLARTE, L. C.
700 1 $aSILVA, M. R.
700 1 $aRODRIGUES, R. A.
700 1 $aCARNEIRO, P. A. M.
700 1 $aKANEENE, J. B.
700 1 $aPASQUATTI, T. N.
700 1 $aTAKATANI, H.
700 1 $aZUMÁRRAGA, M. J.
700 1 $aETGES, R. N.
700 1 $aARAUJO, F. R.
700 1 $aVERBISCK, N. V.
773 $tJournal the Veterinary Medical Science$gv. 81, n. 10, p.1400?1408, 2019.
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Embrapa Gado de Corte (CNPGC) |
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