Data/hora: 18/06/2024 01:28:50

Biblioteca(s): | Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia.
Data corrente: | 10/11/2006
Data da última atualização: | 03/06/2022
Autoria: | FARIA, J. C.; ALBINO, M. M. C.; DIAS, B. B. A.; CANÇADO, L. J.; CUNHA, N. B. da; SILVA, L. de M.; VIANNA, G. R.; ARAGÃO, F. J. L.
Título: | Partial resistance to Bean golden mosaic virus in a transgenic common bean (Phaseolus vulgaris L.) line expressing a mutated rep gene.
Ano de publicação: | 2006
Fonte/Imprenta: | Plant Science, Limerick, v. 171, p. 565-571, 2006.
Idioma: | Inglês
Conteúdo: | The rep gene of Bean golden mosaic virus (BGMV) is essential for virus replication. A mutated rep gene with amino acid codon change in the putative nucleoside triphosphate (NTP) binding motif D262R was created. Phaseolus vulgaris transformation was achieved with a vector that contained the mutated rep and bar genes. A total of 17 initial (T0) transformants were analyzed. One line (M1/4) showed tolerance to glufosinate ammonium and partial resistance to the virus, that is, disease incidence depended on inoculation level. The incidence of BGMVincreased with the increasing number of viruliferous whiteflies per plant, both in the transgenic and in the control plants. However, the number of symptomless plants was significantly higher in the transgenic group. The line M1/4 was studied during several generations and presented stability in the transgene loci and virus resistance. Southern blot analysis with genomic DNA of eight generations led to an estimate of two copies of the rep gene integrated at the same locus. RT-PCR analysis revealed the presence of both bar and rep genes transcripts. The mutated REP protein was present in amounts detectable by Western blot analysis in transgenic plants.
Palavras-Chave: | BGMV; Dry bean; Geminivirus; Rep gene transdominance.
Thesagro: | Feijão; Phaseolus Vulgaris; Planta Transgênica; Resistência; Variedade Resistente; Vírus.

Categoria do assunto:

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X Pesquisa, Tecnologia e Engenharia

URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/178023/1/ID-27510-1.pdf
Registro original: | Embrapa Recursos Genéticos e Biotecnologia (CENARGEN)

Exemplares:

CENARGEN - (27510-1) - UPC - AP - DD - SP 19035 -  - 19035 - DIS
CNPAF - (24404-1) - UPC - AP - PP - 2006 -  - 2006 - DIS