| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Soja. |
|
Data corrente: |
26/08/2014 |
|
Data da última atualização: |
19/06/2017 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
GOMES, D. F.; BATISTA, J. S. da S.; ROLLA, A. A. P.; SILVA, L. P. da; BLOCH, C.; GALLI-TARASAWA, L. V.; HUNGRIA, M. |
|
Afiliação: |
DOUGLAS FABIANO GOMES, Departamento de Génetica UFPR; JESSIANE STEFÂNIA DA SILVA BATISTA, DEBIOGEM UEPG; AMANDA ALVES PAIVA ROLLA; LUCIANO PAULINO DA SILVA, CENARGEN; CARLOS BLOCH JUNIOR, Cenargen; LYGIA VITORIA GALLI TERASAWA, Departamento de Génetica UFPR; MARIANGELA HUNGRIA DA CUNHA, CNPSO. |
|
Título: |
Proteomic analysis of free-living Bradyrhizobium diazoefficiens: highlighting potential determinants of a successful symbiosis |
|
Ano de publicação: |
2014 |
|
Fonte/Imprenta: |
BMC Genomics, v. 15, n. 643, 2014. |
|
DOI: |
10.1186/1471-2164-15-643 |
|
Idioma: |
Português |
|
Conteúdo: |
Strain CPAC 7 (=SEMIA 5080) was recently reclassified into the new species Bradyrhizobium diazoefficiens; due to its outstanding efficiency in fixing nitrogen, it has been used in commercial inoculants for application to crops of soybean [Glycine max (L.) Merr.] in Brazil and other South American countries. Although the efficiency of B. diazoefficiens inoculant strains is well recognized, few data on their protein expression are available. We provided a two-dimensional proteomic reference map of CPAC 7 obtained under free-living conditions, with the successful identification of 115 spots, representing 95 different proteins. The results highlighted the expression of molecular determinants potentially related to symbiosis establishment (e.g. inositol monophosphatase, IMPase), fixation of atmospheric nitrogen (N 2) (e.g. NifH) and defenses against stresses (e.g. chaperones). By using bioinformatic tools, it was possible to attribute probable functions to ten hypothetical proteins. For another ten proteins classified as? NO related COG? group, we analyzed by RT-qPCR the relative expression of their coding-genes in response to the nodulation-gene inducer genistein. Six of these genes were up-regulated, including blr0227, which may be related to polyhydroxybutyrate (PHB) biosynthesis and competitiveness for nodulation. The proteomic map contributed to the identification of several proteins of B. diazoefficiens under free-living conditions and our approach? combining bioinformatics and gene-expression assays ? resulted in new information about unknown genes that might play important roles in the establishment of the symbiosis with soybean. MenosStrain CPAC 7 (=SEMIA 5080) was recently reclassified into the new species Bradyrhizobium diazoefficiens; due to its outstanding efficiency in fixing nitrogen, it has been used in commercial inoculants for application to crops of soybean [Glycine max (L.) Merr.] in Brazil and other South American countries. Although the efficiency of B. diazoefficiens inoculant strains is well recognized, few data on their protein expression are available. We provided a two-dimensional proteomic reference map of CPAC 7 obtained under free-living conditions, with the successful identification of 115 spots, representing 95 different proteins. The results highlighted the expression of molecular determinants potentially related to symbiosis establishment (e.g. inositol monophosphatase, IMPase), fixation of atmospheric nitrogen (N 2) (e.g. NifH) and defenses against stresses (e.g. chaperones). By using bioinformatic tools, it was possible to attribute probable functions to ten hypothetical proteins. For another ten proteins classified as? NO related COG? group, we analyzed by RT-qPCR the relative expression of their coding-genes in response to the nodulation-gene inducer genistein. Six of these genes were up-regulated, including blr0227, which may be related to polyhydroxybutyrate (PHB) biosynthesis and competitiveness for nodulation. The proteomic map contributed to the identification of several proteins of B. diazoefficiens under free-living conditions and our approach? combining bioinformatics... Mostrar Tudo |
|
Palavras-Chave: |
RT-qPCR; Two-dimensional proteomics. |
|
Thesaurus Nal: |
Bradyrhizobium; nitrogen fixation; symbiosis. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/160837/1/12864-2014-Article-6775.pdf
|
|
Marc: |
LEADER 02463naa a2200265 a 4500
001 1993458
005 2017-06-19
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1186/1471-2164-15-643$2DOI
100 1 $aGOMES, D. F.
245 $aProteomic analysis of free-living Bradyrhizobium diazoefficiens$bhighlighting potential determinants of a successful symbiosis$h[electronic resource]
260 $c2014
520 $aStrain CPAC 7 (=SEMIA 5080) was recently reclassified into the new species Bradyrhizobium diazoefficiens; due to its outstanding efficiency in fixing nitrogen, it has been used in commercial inoculants for application to crops of soybean [Glycine max (L.) Merr.] in Brazil and other South American countries. Although the efficiency of B. diazoefficiens inoculant strains is well recognized, few data on their protein expression are available. We provided a two-dimensional proteomic reference map of CPAC 7 obtained under free-living conditions, with the successful identification of 115 spots, representing 95 different proteins. The results highlighted the expression of molecular determinants potentially related to symbiosis establishment (e.g. inositol monophosphatase, IMPase), fixation of atmospheric nitrogen (N 2) (e.g. NifH) and defenses against stresses (e.g. chaperones). By using bioinformatic tools, it was possible to attribute probable functions to ten hypothetical proteins. For another ten proteins classified as? NO related COG? group, we analyzed by RT-qPCR the relative expression of their coding-genes in response to the nodulation-gene inducer genistein. Six of these genes were up-regulated, including blr0227, which may be related to polyhydroxybutyrate (PHB) biosynthesis and competitiveness for nodulation. The proteomic map contributed to the identification of several proteins of B. diazoefficiens under free-living conditions and our approach? combining bioinformatics and gene-expression assays ? resulted in new information about unknown genes that might play important roles in the establishment of the symbiosis with soybean.
650 $aBradyrhizobium
650 $anitrogen fixation
650 $asymbiosis
653 $aRT-qPCR
653 $aTwo-dimensional proteomics
700 1 $aBATISTA, J. S. da S.
700 1 $aROLLA, A. A. P.
700 1 $aSILVA, L. P. da
700 1 $aBLOCH, C.
700 1 $aGALLI-TARASAWA, L. V.
700 1 $aHUNGRIA, M.
773 $tBMC Genomics$gv. 15, n. 643, 2014.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Soja (CNPSO) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Pantanal. Para informações adicionais entre em contato com cpap.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Pantanal. |
|
Data corrente: |
06/07/1999 |
|
Data da última atualização: |
29/03/2017 |
|
Autoria: |
MAURO, R. de A.; TOMAS, W. M. |
|
Afiliação: |
EMBRAPA. Centro de Pesquisa Agropecuaria do Pantanal (Corumba, MS). |
|
Título: |
Avifauna da Estacao Ecologica Nhumirim e adjacencias. |
|
Ano de publicação: |
1994 |
|
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE ZOOLOGIA, 20., 1994, Rio de Janeiro. Resumos. Rio de Janeiro: UFRJ / Instituto de Biologia / Museu Nacional / SBZ, 1994. p.111. |
|
Idioma: |
Português |
|
Conteúdo: |
A Estacao Ecologica Nhumirim (18 59'S; 56 39'W) parte integrante da Faz. Nhumirim (4.310 ha) do Centro de Pesquisa Agropecuaria do Pantanal (CPAP/EMBRAPA), foi criada para constituir uma amostra protegida do pantanal arenoso, na sub-regiao da Nhecolandia. Com 680 ha, a EEN possui as principais fisionomias da sub-regiao: campos naturais, cordilheiras (cordoes arenosos com mata ou cerradao), caapoes (ilhas de cerrado), baias (lagoas com agua doce) e salinas (lagoas com agua salobra). O inventario avifaunistico foi iniciado em 1988. Utilizamos binoculos e a vocalizacao das aves para identificar as especies em rotas pre-determinadas dentro e na periferia da Estacao. As saidas a campo eram realizadas pela manha e final da tarde, sendo eventualmente efetuadas durante a noite para includir na lista as especies de habito equivalente. Foram registradas 243 especies de aves para a Estacao e adjacencias, constituindo35,2% das especies que ocorrem no pantanal. Consta em nossa relacao duas aves de lista de especie da fauna brasileira ameacada de extincao: arara-azul Anodorthynchus hyacinthinus e pica-pau-rei Campephilus robustus. |
|
Palavras-Chave: |
Animal silvestre; Ave; Bird. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 01671naa a2200169 a 4500
001 1797522
005 2017-03-29
008 1994 bl --- 0-- u #d
100 1 $aMAURO, R. de A.
245 $aAvifauna da Estacao Ecologica Nhumirim e adjacencias.
260 $c1994
520 $aA Estacao Ecologica Nhumirim (18 59'S; 56 39'W) parte integrante da Faz. Nhumirim (4.310 ha) do Centro de Pesquisa Agropecuaria do Pantanal (CPAP/EMBRAPA), foi criada para constituir uma amostra protegida do pantanal arenoso, na sub-regiao da Nhecolandia. Com 680 ha, a EEN possui as principais fisionomias da sub-regiao: campos naturais, cordilheiras (cordoes arenosos com mata ou cerradao), caapoes (ilhas de cerrado), baias (lagoas com agua doce) e salinas (lagoas com agua salobra). O inventario avifaunistico foi iniciado em 1988. Utilizamos binoculos e a vocalizacao das aves para identificar as especies em rotas pre-determinadas dentro e na periferia da Estacao. As saidas a campo eram realizadas pela manha e final da tarde, sendo eventualmente efetuadas durante a noite para includir na lista as especies de habito equivalente. Foram registradas 243 especies de aves para a Estacao e adjacencias, constituindo35,2% das especies que ocorrem no pantanal. Consta em nossa relacao duas aves de lista de especie da fauna brasileira ameacada de extincao: arara-azul Anodorthynchus hyacinthinus e pica-pau-rei Campephilus robustus.
653 $aAnimal silvestre
653 $aAve
653 $aBird
700 1 $aTOMAS, W. M.
773 $tIn: CONGRESSO BRASILEIRO DE ZOOLOGIA, 20., 1994, Rio de Janeiro. Resumos. Rio de Janeiro: UFRJ / Instituto de Biologia / Museu Nacional / SBZ, 1994. p.111.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Pantanal (CPAP) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
