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Registro Completo |
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
15/12/2015 |
Data da última atualização: |
20/03/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ARCANJO, D. D. R.; VASCONCELOS, A. G.; COMERMA-STEFFENSEN, S. G.; JESUS, J. R.; SILVA, L. P. da; PIRES JÚNIOR, O. R.; COSTA-NETO, C. M.; OLIVEIRA, E. B.; MIGLIOLO, L.; FRANCO, O. L.; RESTINI, C. B. A.; PAULO, M.; BENDHACK, L. M.; BEMQUERER, M. P.; OLIVEIRA, A. P.; SIMONSEN, U.; LEITE, J. R. de S. de A. |
Afiliação: |
DANIEL DIAS RUFINO ARCANJO, Universidade Federal do Piauí –UFPI; ANDREANNE GOMES VASCONCELOS, Universidade Federal do Piauí –UFPI; SIMÓN GABRIEL, Aarhus University; JOILSON RAMOS JESUS, Universidade Federal do Piauí –UFPI; LUCIANO PAULINO DA SILVA, CENARGEN; OSMINDO RODRIGUES PIRES JÚNIOR, Universidade de Brasília–UnB; CLAUDIO MIGUEL COSTA-NETO, Universidade de São Paulo–USP, Ribeirão Preto; EDUARDO BRANDT OLIVEIRA, Universidade de São Paulo–USP, Ribeirão Preto; LUDOVICO MIGLIOLO, Universidade Católica de Brasília–UCB; OCTÁVIO LUIZ FRANCO, Universidade Católica de Brasília–UCB; CAROLINA BARALDI ARAÚJO RESTINI, Universidade de Ribeirão Preto–UNAERP; MICHELE PAULO, Universidade de São Paulo–USP, Ribeirão Preto; LUSIANE MARIA BENDHACK, Universidade de São Paulo–USP, Ribeirão Preto; MARCELO PORTO BEMQUERER, CENARGEN; ALDEIDIA PEREIRA OLIVEIRA, Universidade Federal do Piauí –UFPI; ULF SIMONSEN, Aarhus University; JOSÉ ROBERTO DE SOUZA DE ALMEIDA LEITE, Universidade Federal do Piauí –UFPI. |
Título: |
A novel vasoactive proline-rich oligopeptide from the skin secretion of the frog Brachycephalus ephippium. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Plos One, v. 10, dez. 2015. (Open Access) |
DOI: |
10.1371/journal.pone.0145071 |
Idioma: |
Inglês |
Conteúdo: |
Proline-rich oligopeptides (PROs) are a large family which comprises the bradykinin-potentiating peptides (BPPs). They inhibit the activity of the angiotensin I-converting enzyme (ACE) and have a typical pyroglutamyl (Pyr)/proline-rich structure at the N- and C-terminus, respectively. Furthermore, PROs decrease blood pressure in animals. In the present study, the isolation and biological characterization of a novel vasoactive BPP isolated from the skin secretion of the frog Brachycephalus ephippium is described. This new PRO, termed BPP-Brachy, has the primary structure WPPPKVSP and the amidated form termed BPPBrachyNH2 inhibits efficiently ACE in rat serum. In silico molecular modeling and docking studies suggest that BPP-BrachyNH2 is capable of forming a hydrogen bond network as well as multiple van der Waals interactions with the rat ACE, which blocks the access of the substrate to the C-domain active site. Moreover, in rat thoracic aorta BPP-BrachyNH2 induces potent endothelium-dependent vasodilatation with similar magnitude as captopril. In DAF-FM DA-loaded aortic cross sections examined by confocal microscopy, BPP-BrachyNH2 was found to increase the release of nitric oxide (NO). Moreover, BPP-BrachyNH2 was devoid of toxicity in endothelial and smooth muscle cell cultures. In conclusion, the peptide BPP-BrachyNH2 has a novel sequence being the first BPP isolated from the skin secretion of the Brachycephalidae family. This opens for exploring amphibians as a source of new biomolecules. The BPP-BrachyNH2 is devoid of cytotoxicity and elicits endothelium-dependent vasodilatation mediated by NO. These findings open for the possibility of potential application of these peptides in the treatment of endothelial dysfunction and cardiovascular diseases. MenosProline-rich oligopeptides (PROs) are a large family which comprises the bradykinin-potentiating peptides (BPPs). They inhibit the activity of the angiotensin I-converting enzyme (ACE) and have a typical pyroglutamyl (Pyr)/proline-rich structure at the N- and C-terminus, respectively. Furthermore, PROs decrease blood pressure in animals. In the present study, the isolation and biological characterization of a novel vasoactive BPP isolated from the skin secretion of the frog Brachycephalus ephippium is described. This new PRO, termed BPP-Brachy, has the primary structure WPPPKVSP and the amidated form termed BPPBrachyNH2 inhibits efficiently ACE in rat serum. In silico molecular modeling and docking studies suggest that BPP-BrachyNH2 is capable of forming a hydrogen bond network as well as multiple van der Waals interactions with the rat ACE, which blocks the access of the substrate to the C-domain active site. Moreover, in rat thoracic aorta BPP-BrachyNH2 induces potent endothelium-dependent vasodilatation with similar magnitude as captopril. In DAF-FM DA-loaded aortic cross sections examined by confocal microscopy, BPP-BrachyNH2 was found to increase the release of nitric oxide (NO). Moreover, BPP-BrachyNH2 was devoid of toxicity in endothelial and smooth muscle cell cultures. In conclusion, the peptide BPP-BrachyNH2 has a novel sequence being the first BPP isolated from the skin secretion of the Brachycephalidae family. This opens for exploring amphibians as a source of ne... Mostrar Tudo |
Palavras-Chave: |
Brachycephalus ephippium; Proline-rich oligopeptides. |
Thesaurus Nal: |
secretion. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/182309/1/journal.pone.0145071.PDF
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Marc: |
LEADER 02864naa a2200361 a 4500 001 2031700 005 2023-03-20 008 2015 bl uuuu u00u1 u #d 024 7 $a10.1371/journal.pone.0145071$2DOI 100 1 $aARCANJO, D. D. R. 245 $aA novel vasoactive proline-rich oligopeptide from the skin secretion of the frog Brachycephalus ephippium.$h[electronic resource] 260 $c2015 520 $aProline-rich oligopeptides (PROs) are a large family which comprises the bradykinin-potentiating peptides (BPPs). They inhibit the activity of the angiotensin I-converting enzyme (ACE) and have a typical pyroglutamyl (Pyr)/proline-rich structure at the N- and C-terminus, respectively. Furthermore, PROs decrease blood pressure in animals. In the present study, the isolation and biological characterization of a novel vasoactive BPP isolated from the skin secretion of the frog Brachycephalus ephippium is described. This new PRO, termed BPP-Brachy, has the primary structure WPPPKVSP and the amidated form termed BPPBrachyNH2 inhibits efficiently ACE in rat serum. In silico molecular modeling and docking studies suggest that BPP-BrachyNH2 is capable of forming a hydrogen bond network as well as multiple van der Waals interactions with the rat ACE, which blocks the access of the substrate to the C-domain active site. Moreover, in rat thoracic aorta BPP-BrachyNH2 induces potent endothelium-dependent vasodilatation with similar magnitude as captopril. In DAF-FM DA-loaded aortic cross sections examined by confocal microscopy, BPP-BrachyNH2 was found to increase the release of nitric oxide (NO). Moreover, BPP-BrachyNH2 was devoid of toxicity in endothelial and smooth muscle cell cultures. In conclusion, the peptide BPP-BrachyNH2 has a novel sequence being the first BPP isolated from the skin secretion of the Brachycephalidae family. This opens for exploring amphibians as a source of new biomolecules. The BPP-BrachyNH2 is devoid of cytotoxicity and elicits endothelium-dependent vasodilatation mediated by NO. These findings open for the possibility of potential application of these peptides in the treatment of endothelial dysfunction and cardiovascular diseases. 650 $asecretion 653 $aBrachycephalus ephippium 653 $aProline-rich oligopeptides 700 1 $aVASCONCELOS, A. G. 700 1 $aCOMERMA-STEFFENSEN, S. G. 700 1 $aJESUS, J. R. 700 1 $aSILVA, L. P. da 700 1 $aPIRES JÚNIOR, O. R. 700 1 $aCOSTA-NETO, C. M. 700 1 $aOLIVEIRA, E. B. 700 1 $aMIGLIOLO, L. 700 1 $aFRANCO, O. L. 700 1 $aRESTINI, C. B. A. 700 1 $aPAULO, M. 700 1 $aBENDHACK, L. M. 700 1 $aBEMQUERER, M. P. 700 1 $aOLIVEIRA, A. P. 700 1 $aSIMONSEN, U. 700 1 $aLEITE, J. R. de S. de A. 773 $tPlos One$gv. 10, dez. 2015. (Open Access)
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registro Completo
Biblioteca(s): |
Embrapa Caprinos e Ovinos; Embrapa Pecuária Sudeste. |
Data corrente: |
23/05/2023 |
Data da última atualização: |
28/11/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
DIAS, J. H.; VERGANI; GONÇALVES, J. D.; OLIVEIRA, T. A.; PENITENTE-FILHO, J. M.; PEREIRA, V. S. do A.; ESTEVES, S. N.; GARCIA, A. R.; BATISTA, R. I. T. P.; OLIVEIRA, M. E. F.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da. |
Afiliação: |
J. H. DIAS, Universidade Federal de Viçosa; G. B. VERGANI, Universidade Estadual Paulista; J. D. GONÇALVES, Universidade Estadual Paulista; T. A. OLIVEIRA, Universidade Federal Fluminense; J. M. PENITENTE-FILHO, Universidade Federal de Viçosa; VERONICA SCHINAIDER DO A PEREIRA, CPPSE; SERGIO NOVITA ESTEVES, CPPSE; ALEXANDRE ROSSETTO GARCIA, CPPSE; R. I. T. P. BATISTA, Universidade Federal Fluminense; M. E. F. OLIVEIRA, Universidade Estadual Paulista; J. M. G. SOUZA-FABJAN, Universidade Federal Fluminense; JEFERSON FERREIRA DA FONSECA, CNPC. |
Título: |
Different doses of pFSH are effective to promote follicular growth, superovulatory response, and embryo yield in White Dorper ewes. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Small Ruminant Research, v. 220, n. 103, mar. 2023, 106914. |
DOI: |
https://doi.org/10.1016/j.smallrumres.2023.106914 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: This study evaluated the effect of 250, 333, or 400 IU of a commercial porcine stimulating-follicle hormone (pFSH) on follicular growth, superovulatory (SOV) response, and embryo yield in White Dorper sheep. Ewes received an induced-ovulation protocol (D0 = progesterone (P4) device insertion) and superovulation starting 60 h before P4 withdrawal (D9), which consisted of six decreasing doses of 250 IU (G250; n = 15), 333 IU (G333; n = 15), or 400 IU (G400; n = 14) of pFSH. Ewes also received 50 µg of gonadotropin-releasing hormone (GnRH) analog on D10 and 300 IU of hCG on D13. Ewes were mated and submitted to non-surgical embryo recovery (NSER). Ultrasound evaluations were performed on D6, D7, D8, D9, and D16. The overall responding donor rate (≥3 CL) was 86.4% (38/44). The mean number of large follicles was higher (P < 0.05) in all groups on D8 (6.2 ± 0.9, 7.7 ± 0.9, and 8.3 ± 1.1) and D9 (11.1 ± 0.6, 10.4 ± 1.0, and 11.5 ± 0.9). Non-surgical embryo recovery procedures were successfully performed in 60.5% (23/38) of females. The CL count in successfully flushed ewes was greater (P < 0.05) in G333 (12.7 ± 2.3) and G400 (12.0 ± 1.3) than in G250 (6.0 ± 1.1). The recovery rate (44.4%, 57.3%, and 50.0%) and numbers of transferable embryos (2.3 ± 1.1, 6.1 ± 2.6, and 4.5 ± 1.3) did not differ (P > 0.05) among groups. All protocols were efficient in promoting follicular growth and adequate superovulatory response and embryo yield. |
Palavras-Chave: |
Follicular dynamics; FSH; MOET; Ovarian superestimulation; Ovine; PFSH. |
Thesaurus NAL: |
Ewes; Reproduction; Sheep. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02594naa a2200373 a 4500 001 2153917 005 2023-11-28 008 2023 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.smallrumres.2023.106914$2DOI 100 1 $aDIAS, J. H. 245 $aDifferent doses of pFSH are effective to promote follicular growth, superovulatory response, and embryo yield in White Dorper ewes.$h[electronic resource] 260 $c2023 520 $aAbstract: This study evaluated the effect of 250, 333, or 400 IU of a commercial porcine stimulating-follicle hormone (pFSH) on follicular growth, superovulatory (SOV) response, and embryo yield in White Dorper sheep. Ewes received an induced-ovulation protocol (D0 = progesterone (P4) device insertion) and superovulation starting 60 h before P4 withdrawal (D9), which consisted of six decreasing doses of 250 IU (G250; n = 15), 333 IU (G333; n = 15), or 400 IU (G400; n = 14) of pFSH. Ewes also received 50 µg of gonadotropin-releasing hormone (GnRH) analog on D10 and 300 IU of hCG on D13. Ewes were mated and submitted to non-surgical embryo recovery (NSER). Ultrasound evaluations were performed on D6, D7, D8, D9, and D16. The overall responding donor rate (≥3 CL) was 86.4% (38/44). The mean number of large follicles was higher (P < 0.05) in all groups on D8 (6.2 ± 0.9, 7.7 ± 0.9, and 8.3 ± 1.1) and D9 (11.1 ± 0.6, 10.4 ± 1.0, and 11.5 ± 0.9). Non-surgical embryo recovery procedures were successfully performed in 60.5% (23/38) of females. The CL count in successfully flushed ewes was greater (P < 0.05) in G333 (12.7 ± 2.3) and G400 (12.0 ± 1.3) than in G250 (6.0 ± 1.1). The recovery rate (44.4%, 57.3%, and 50.0%) and numbers of transferable embryos (2.3 ± 1.1, 6.1 ± 2.6, and 4.5 ± 1.3) did not differ (P > 0.05) among groups. All protocols were efficient in promoting follicular growth and adequate superovulatory response and embryo yield. 650 $aEwes 650 $aReproduction 650 $aSheep 653 $aFollicular dynamics 653 $aFSH 653 $aMOET 653 $aOvarian superestimulation 653 $aOvine 653 $aPFSH 700 1 $aVERGANI 700 1 $aGONÇALVES, J. D. 700 1 $aOLIVEIRA, T. A. 700 1 $aPENITENTE-FILHO, J. M. 700 1 $aPEREIRA, V. S. do A. 700 1 $aESTEVES, S. N. 700 1 $aGARCIA, A. R. 700 1 $aBATISTA, R. I. T. P. 700 1 $aOLIVEIRA, M. E. F. 700 1 $aSOUZA-FABJAN, J. M. G. 700 1 $aFONSECA, J. F. da 773 $tSmall Ruminant Research$gv. 220, n. 103, mar. 2023, 106914.
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