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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
08/02/2018 |
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Data da última atualização: |
09/08/2021 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
PINTO, A. C. G. R.; RODRIGUES, J. P.; PETI, A. P. F.; SILVA, L. H. F.; MELO, I. S. de; MORAES, L. A. B. |
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Afiliação: |
A. C. G. R. PINTO; J. P. RODRIGUES; A. P. F. PETI; L. H. F. SILVA; ITAMAR SOARES DE MELO, CNPMA; L. A. B. MORAES. |
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Título: |
Optimization of actinomycin D production using response surface methodology as statistical tool. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
In: BRAZILIAN CONFERENCE ON NATURAL PRODUCTS, 6.; ANUAL MEETING ON MICROMOLECULAR EVOLUTION, SYSTEMATICS AND ECOLOGY, 32., 2017. Vitória. Annals... Vitória: UFES, 2017. |
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Idioma: |
Português |
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Conteúdo: |
In a general way, the isolated microbial strains from nature produce discrete amounts of a particular secondary metabolite. The identification and characterization of bioactive compounds from crude extracts, makes it possible to improve the production of a metabolite of interest. The Streptomyces sp strain Caat 7-38, isolated from Caatinga biome rhizosphere which produces Actinomycin D, a potent antibiotic marketed under an antitumor agent. In order to improve the production of Actinomycin D, an experimental design 3¬k (33), generating response surfaces as a function of the independent variables: carbon source (%), nitrogen source (%) and temperature (°C) of culture conditions in ISP-2 medium, and as a dependent variable (response), the production of actinomycin D, was performed. The crude extracts generated in each one of the experiments were analyzed and processed by LC-MS with constructing an analytical curve with standard solution of 10, 50, 100, 200(?g/mL), and thus, the determination of the concentration of the compound in the different crude extracts. Through experimental results it was possible to obtain an empirical mathematical model that was able predicting the studied system, and thus, to determine the optimal values for each independent variable under the study ranges. With the validation of mathematical model and optimal values, we increased the production of Actinomycin D in 5-fold in regarding to control (standard growth media). |
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Thesagro: |
Estatística. |
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Thesaurus Nal: |
Actinomycin D. |
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Categoria do assunto: |
V Taxonomia de Organismos |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/217167/1/2017SP-Pinto-Optimization-16035.pdf
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Marc: |
LEADER 02185nam a2200193 a 4500
001 2087495
005 2021-08-09
008 2017 bl uuuu u00u1 u #d
100 1 $aPINTO, A. C. G. R.
245 $aOptimization of actinomycin D production using response surface methodology as statistical tool.$h[electronic resource]
260 $aIn: BRAZILIAN CONFERENCE ON NATURAL PRODUCTS, 6.; ANUAL MEETING ON MICROMOLECULAR EVOLUTION, SYSTEMATICS AND ECOLOGY, 32., 2017. Vitória. Annals... Vitória: UFES$c2017
520 $aIn a general way, the isolated microbial strains from nature produce discrete amounts of a particular secondary metabolite. The identification and characterization of bioactive compounds from crude extracts, makes it possible to improve the production of a metabolite of interest. The Streptomyces sp strain Caat 7-38, isolated from Caatinga biome rhizosphere which produces Actinomycin D, a potent antibiotic marketed under an antitumor agent. In order to improve the production of Actinomycin D, an experimental design 3¬k (33), generating response surfaces as a function of the independent variables: carbon source (%), nitrogen source (%) and temperature (°C) of culture conditions in ISP-2 medium, and as a dependent variable (response), the production of actinomycin D, was performed. The crude extracts generated in each one of the experiments were analyzed and processed by LC-MS with constructing an analytical curve with standard solution of 10, 50, 100, 200(?g/mL), and thus, the determination of the concentration of the compound in the different crude extracts. Through experimental results it was possible to obtain an empirical mathematical model that was able predicting the studied system, and thus, to determine the optimal values for each independent variable under the study ranges. With the validation of mathematical model and optimal values, we increased the production of Actinomycin D in 5-fold in regarding to control (standard growth media).
650 $aActinomycin D
650 $aEstatística
700 1 $aRODRIGUES, J. P.
700 1 $aPETI, A. P. F.
700 1 $aSILVA, L. H. F.
700 1 $aMELO, I. S. de
700 1 $aMORAES, L. A. B.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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| Registros recuperados : 3 | |
| 1. |  | SANTOS, M. M. P.; DAHER, R. F.; PONCIANO, N. J.; GRAVINA, G. A.; PEREIRA, A. V.; SANTOS, C. L. Respostas do capim-elefante sob doses de adubação azotada de cobertura para fins energéticos. Revista de Ciências Agrárias, v. 37, n. 1, p. 100-108, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 2. | | SANTOS, M. M. P.; PONCIANO, N. J.; DAHER, R. F.; GRAVINA, G. A.; PEREIRA, A. V.; SANTOS, C. L. Otimização azotada no Capim-elefante para fins energéticos. Revista de Ciências Agrárias, Lisboa, v. 39, p. 366-375, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 3. | | SANTOS, M. M. P.; DAHER, R. F.; PONCIANO, N. J.; GRAVINA, G. de A.; PEREIRA, A. V.; SANT'ANA, J. A. de A.; SANTOS, C. L. Características produtivas de capim-elefante sob doses de fósforo e nitrogênio para fins energéticos. Científica - Revista de Ciências Agrárias, v. 42, n. 4, p. 354-365, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 3 | |
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