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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
26/02/2007 |
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Data da última atualização: |
23/03/2007 |
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Autoria: |
ALMEIDA NETO, J. B. de; SÁ, F B. de; SILVA, K. P. C.; BUZINHANI, M. |
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Título: |
Flora conjuntival bacteriana de ovinos sadios da raça Santa Inês e seus mestiços criados na microrregião de Garanhuns, Pernambuco. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
Ciência Veterinária nos Trópicos, Recife, v. 9, n. 1, p. 17-22, 2006. |
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Idioma: |
Português |
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Conteúdo: |
Em todos os animais existe uma flora bacteriana conjuntival normal. Para correta avaliaçao dos resultados de qualquer tecnica de cultura, e necessaria conhecer os microrganismos considerados como parte da flora normal, diferenciando-os dos patogenicos. Neste estudo o objetivo foi estabelecer padroes bacteriologicos de sacos conjuntivais de ovinos sadios. Avaliou-se 60 olhos de 30 animais sem alteracoes clinicas aparentes. Para o isolamento bacteriano e pesquisa de Mycoplasma, utilizou-se swab do saco conjuntival. Moraxella sp. foi a bacteria mais isolada, presente em 28,33% (17/60) dos olhos sadios, seguido par Staphylococcus sp., 23,33% (14/60). Streptococcus sp., 5,00% (3/60), Micrococcus sp" 5,00% (3/60), Corynebacterium sp., 3,33% (2/60), Bacillus sp" 3,33% (2/60) e Serratia sp., 1,67% (1/60). 0 Mycoplasma conjunctivae esteve presente em 15,00% (9/60) das amostras estudadas. |
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Palavras-Chave: |
Brasil; Controle bacteriologico; Moraxella sp; Pernambuco. |
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Thesagro: |
Infecção; Microbiologia; Olho; Ovino. |
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Thesaurus Nal: |
Mycoplasma conjunctivae. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01681naa a2200265 a 4500
001 1532926
005 2007-03-23
008 2006 bl uuuu u00u1 u #d
100 1 $aALMEIDA NETO, J. B. de
245 $aFlora conjuntival bacteriana de ovinos sadios da raça Santa Inês e seus mestiços criados na microrregião de Garanhuns, Pernambuco.
260 $c2006
520 $aEm todos os animais existe uma flora bacteriana conjuntival normal. Para correta avaliaçao dos resultados de qualquer tecnica de cultura, e necessaria conhecer os microrganismos considerados como parte da flora normal, diferenciando-os dos patogenicos. Neste estudo o objetivo foi estabelecer padroes bacteriologicos de sacos conjuntivais de ovinos sadios. Avaliou-se 60 olhos de 30 animais sem alteracoes clinicas aparentes. Para o isolamento bacteriano e pesquisa de Mycoplasma, utilizou-se swab do saco conjuntival. Moraxella sp. foi a bacteria mais isolada, presente em 28,33% (17/60) dos olhos sadios, seguido par Staphylococcus sp., 23,33% (14/60). Streptococcus sp., 5,00% (3/60), Micrococcus sp" 5,00% (3/60), Corynebacterium sp., 3,33% (2/60), Bacillus sp" 3,33% (2/60) e Serratia sp., 1,67% (1/60). 0 Mycoplasma conjunctivae esteve presente em 15,00% (9/60) das amostras estudadas.
650 $aMycoplasma conjunctivae
650 $aInfecção
650 $aMicrobiologia
650 $aOlho
650 $aOvino
653 $aBrasil
653 $aControle bacteriologico
653 $aMoraxella sp
653 $aPernambuco
700 1 $aSÁ, F B. de
700 1 $aSILVA, K. P. C.
700 1 $aBUZINHANI, M.
773 $tCiência Veterinária nos Trópicos, Recife$gv. 9, n. 1, p. 17-22, 2006.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
06/10/2021 |
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Data da última atualização: |
10/06/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
IWANICKI, N. S.; MASCARIN, G. M.; MORENO, S. G.; EILENBERG, J.; DELALIBERA JUNIOR, I. |
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Afiliação: |
NATASHA SANT´ANNA IWANICKI, ESALQ-USP; GABRIEL MOURA MASCARIN, CNPMA; SARA GIRO MORENO, ESALQ-USP; JØRGEN EILENBERG, University of Copenhagen; ITALO DELALIBERA JUNIOR, ESALQ-USP. |
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Título: |
Development of novel spray-dried and air-dried formulations of Metarhizium robertsii blastospores and their virulence. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Applied Microbiology and Biotechnology, v. 105, n. 20, p. 7913-7933, 2021. |
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ISSN: |
1432-0614 |
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DOI: |
https://doi.org/10.1007/s00253-021-11576-5 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The present research addressed spray-drying and air-drying techniques applied to Metarhizium robertsii blastospores to develop wettable powder (WP) formulations. We investigated the effect of co-formulants on blastospore viability during drying and assessed the wettability and stability of formulations in water. The effect of oxygen-moisture absorbers was studied on the shelf life of these formulations stored at 26 °C and 4 °C for up to 90 days. Additionally, we determined the virulence of the best spray-dried and air-dried formulations against the corn leafhopper Dalbulus maidis. While sucrose and skim milk played an essential role as osmoprotectants in preserving air-dried blastospores, maltodextrin, skim milk, and bentonite were crucial to attain high cell survival during spray drying. The lowest wettability time was achieved with spray-dried formulations containing less Ca-lignin, while charcoal powder amount was positively associated with formulation stability. The addition of oxygen-moisture absorbers inside sealed packages increased from threefold to fourfold the half-life times of air-dried and spray-dried formulations at both storage temperatures. However, the half-life times of all blastospore-based formulations were shorter than 3 months regardless of temperature and packaging system. Spray-dried and air-dried WP formulations were as virulent as fresh blastopores against D. maydis adults sprayed with 5 × 107 blastospores mL−1 that induced 87.8% and 70.6% mortality, respectively. These findings bring innovative advancement for M. robertsii blastospore formulation through spray-drying and underpin the importance of adding protective matrices coupled to oxygen-moisture absorbers to extend cell viability during either cold or non-refrigerated storage. MenosAbstract: The present research addressed spray-drying and air-drying techniques applied to Metarhizium robertsii blastospores to develop wettable powder (WP) formulations. We investigated the effect of co-formulants on blastospore viability during drying and assessed the wettability and stability of formulations in water. The effect of oxygen-moisture absorbers was studied on the shelf life of these formulations stored at 26 °C and 4 °C for up to 90 days. Additionally, we determined the virulence of the best spray-dried and air-dried formulations against the corn leafhopper Dalbulus maidis. While sucrose and skim milk played an essential role as osmoprotectants in preserving air-dried blastospores, maltodextrin, skim milk, and bentonite were crucial to attain high cell survival during spray drying. The lowest wettability time was achieved with spray-dried formulations containing less Ca-lignin, while charcoal powder amount was positively associated with formulation stability. The addition of oxygen-moisture absorbers inside sealed packages increased from threefold to fourfold the half-life times of air-dried and spray-dried formulations at both storage temperatures. However, the half-life times of all blastospore-based formulations were shorter than 3 months regardless of temperature and packaging system. Spray-dried and air-dried WP formulations were as virulent as fresh blastopores against D. maydis adults sprayed with 5 × 107 blastospores mL−1 that induc... Mostrar Tudo |
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Palavras-Chave: |
Convective drying. |
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Thesagro: |
Cigarrinha; Fermentação; Formulação; Fungo Entomógeno; Fungo Para Controle Biológico; Pó Molhável; Praga de Planta. |
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Thesaurus NAL: |
Biological control agents; Dalbulus maidis; Entomopathogenic fungi; Liquid state fermentation; Metarhizium robertsii; Pesticide formulations; Wettable powders. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 03008naa a2200373 a 4500
001 2135109
005 2022-06-10
008 2021 bl uuuu u00u1 u #d
022 $a1432-0614
024 7 $ahttps://doi.org/10.1007/s00253-021-11576-5$2DOI
100 1 $aIWANICKI, N. S.
245 $aDevelopment of novel spray-dried and air-dried formulations of Metarhizium robertsii blastospores and their virulence.$h[electronic resource]
260 $c2021
520 $aAbstract: The present research addressed spray-drying and air-drying techniques applied to Metarhizium robertsii blastospores to develop wettable powder (WP) formulations. We investigated the effect of co-formulants on blastospore viability during drying and assessed the wettability and stability of formulations in water. The effect of oxygen-moisture absorbers was studied on the shelf life of these formulations stored at 26 °C and 4 °C for up to 90 days. Additionally, we determined the virulence of the best spray-dried and air-dried formulations against the corn leafhopper Dalbulus maidis. While sucrose and skim milk played an essential role as osmoprotectants in preserving air-dried blastospores, maltodextrin, skim milk, and bentonite were crucial to attain high cell survival during spray drying. The lowest wettability time was achieved with spray-dried formulations containing less Ca-lignin, while charcoal powder amount was positively associated with formulation stability. The addition of oxygen-moisture absorbers inside sealed packages increased from threefold to fourfold the half-life times of air-dried and spray-dried formulations at both storage temperatures. However, the half-life times of all blastospore-based formulations were shorter than 3 months regardless of temperature and packaging system. Spray-dried and air-dried WP formulations were as virulent as fresh blastopores against D. maydis adults sprayed with 5 × 107 blastospores mL−1 that induced 87.8% and 70.6% mortality, respectively. These findings bring innovative advancement for M. robertsii blastospore formulation through spray-drying and underpin the importance of adding protective matrices coupled to oxygen-moisture absorbers to extend cell viability during either cold or non-refrigerated storage.
650 $aBiological control agents
650 $aDalbulus maidis
650 $aEntomopathogenic fungi
650 $aLiquid state fermentation
650 $aMetarhizium robertsii
650 $aPesticide formulations
650 $aWettable powders
650 $aCigarrinha
650 $aFermentação
650 $aFormulação
650 $aFungo Entomógeno
650 $aFungo Para Controle Biológico
650 $aPó Molhável
650 $aPraga de Planta
653 $aConvective drying
700 1 $aMASCARIN, G. M.
700 1 $aMORENO, S. G.
700 1 $aEILENBERG, J.
700 1 $aDELALIBERA JUNIOR, I.
773 $tApplied Microbiology and Biotechnology$gv. 105, n. 20, p. 7913-7933, 2021.
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