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Registro Completo |
Biblioteca(s): |
Embrapa Uva e Vinho. |
Data corrente: |
27/06/2017 |
Data da última atualização: |
06/05/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SILVA, J. M. F; RWAHNIH, M. A.; BLAWID, R.; NAGATA, T.; FAJARDO, T. V. M. |
Afiliação: |
João Marcos Fagundes Silva, University of Bras?lia, Bras?lia, DF, Brazil; Maher Al Rwahnih, Department of Plant Pathology, University of California, Davis, CA, USA; Rosana Blawid, University of Bras? ? lia, Bras? ? lia, DF, Brazil; Tatsuya Nagata, University of Bras?lia, Bras?lia, DF, Brazil; THOR VINICIUS MARTINS FAJARDO, CNPUV. |
Título: |
Discovery and molecular characterization of a novel enamovirus, Grapevine enamovirus-1. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Virus Genes, v.53, n. 4, p. 667-671 [online], 2017. |
DOI: |
10.1007/s11262-017-1470-y |
Idioma: |
Inglês |
Conteúdo: |
In this study, we describe a novel putative Enamovirus member, Grapevine enamovirus-1 (GEV-1), discovered by high-throughput sequencing (HTS). A limited survey using HTS of 17 grapevines (Vitis spp.) from the south, southeast, and northeast regions of Brazil led to the detection of GEV-1 exclusively on southern plants, infecting four grapevine cultivars (Cabernet Sauvignon, Semillon, CG 90450, and Cabernet franc) with a remark-able identity of around 99% at the nucleotide level. This novel virus was only detected in multiple-virus infected plants exhibiting viral-like symptoms. GEV-1 was also detected on a cv. Malvasia Longa by RT-PCR. We performed graft-transmissibility assays on GEV-1. The organization, products, and cis-acting regulatory elements of GEV-1 genome are also discussed here. The near complete genome sequence of GEV-1 was obtained during the course of this study, lacking only part of the 3 0 untranslated terminal region. This is the first report of a virus in the family Luteoviridae infecting grapevines. Based on its genomic properties and phylogenetic analyses, GEV-1 should be classified as a new member of the genus Enamovirus . Keywords High-throughput sequencing Virus discovery Luteoviridae Grapevine enamovirus-1 GEV-1 |
Palavras-Chave: |
GEV-1; Grapevine enamovirus-1; High-throughput sequencing. |
Thesaurus Nal: |
Luteoviridae. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/161127/1/GEV1-Virus-Genes-Silva-et-al-published-June-03-2017.pdf
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Marc: |
LEADER 01940naa a2200229 a 4500 001 2071596 005 2019-05-06 008 2017 bl uuuu u00u1 u #d 024 7 $a10.1007/s11262-017-1470-y$2DOI 100 1 $aSILVA, J. M. F 245 $aDiscovery and molecular characterization of a novel enamovirus, Grapevine enamovirus-1.$h[electronic resource] 260 $c2017 520 $aIn this study, we describe a novel putative Enamovirus member, Grapevine enamovirus-1 (GEV-1), discovered by high-throughput sequencing (HTS). A limited survey using HTS of 17 grapevines (Vitis spp.) from the south, southeast, and northeast regions of Brazil led to the detection of GEV-1 exclusively on southern plants, infecting four grapevine cultivars (Cabernet Sauvignon, Semillon, CG 90450, and Cabernet franc) with a remark-able identity of around 99% at the nucleotide level. This novel virus was only detected in multiple-virus infected plants exhibiting viral-like symptoms. GEV-1 was also detected on a cv. Malvasia Longa by RT-PCR. We performed graft-transmissibility assays on GEV-1. The organization, products, and cis-acting regulatory elements of GEV-1 genome are also discussed here. The near complete genome sequence of GEV-1 was obtained during the course of this study, lacking only part of the 3 0 untranslated terminal region. This is the first report of a virus in the family Luteoviridae infecting grapevines. Based on its genomic properties and phylogenetic analyses, GEV-1 should be classified as a new member of the genus Enamovirus . Keywords High-throughput sequencing Virus discovery Luteoviridae Grapevine enamovirus-1 GEV-1 650 $aLuteoviridae 653 $aGEV-1 653 $aGrapevine enamovirus-1 653 $aHigh-throughput sequencing 700 1 $aRWAHNIH, M. A. 700 1 $aBLAWID, R. 700 1 $aNAGATA, T. 700 1 $aFAJARDO, T. V. M. 773 $tVirus Genes$gv.53, n. 4, p. 667-671 [online], 2017.
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Embrapa Uva e Vinho (CNPUV) |
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1. | | SERRA, L. A.; CRUZ, R. G. da S.; GUTIERREZ, D. M. R.; CRUZ, A. J. G.; CANIZARES, C. A. T.; CHEN, X.; MOSIER, N.; THOMPSON, D.; ASTON, J.; DOOLEY, J.; SHARMA, P.; DE MARCO, J. L.; ALMEIDA, J. R. M. de; ERK, K.; XIMENES, E.; LADISCH, M. R. Screening method for Enzyme-based liquefaction of corn stover pellets at high solids. Bioresource Technology, v. 363, 127999, Nov., 2022.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
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