Data/hora: 01/06/2024 00:52:11

Biblioteca(s): 	Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Unidades Centrais.	
Data corrente: 	22/01/2024	
Data da última atualização: 	02/02/2024	
Autoria: 	MAGARELLI, G.; SILVA, J. G. da; RIBEIRO, C. L.; FREITAS, T. V. de; RODRIGUES, M. A.; GIL, E. de S.; MARRACCINI, P.; SOUZA, J. R. de; CASTRO, C. S. P. de; BEMQUERER, M. P.	
Afiliação: 	GABRIELLA MAGARELLI, Cenargen; JONATAS GOMES DA SILVA, UNIVERSIDADE FEDERAL DO OESTE DA BAHIA; CAROLINE LUCHTENBERG RIBEIRO; THIAGO VIANA DE FREITAS; MAGALI APARECIDA RODRIGUES, UNIVERSIDADE DE SAO PAULO; ERIC DE SOUZA GIL, UNIVERSIDADE FEDERAL DE GOIAS; PIERRE MARRACCINI, CIRAD; JURANDIR RODRIGUES DE SOUZA, UNIVERSIDADE DE BRASÍLIA; CLARISSA SILVA PIRES DE CASTRO, GIS; MARCELO PORTO BEMQUERER, CNPGL.	
Título: 	A voltammetric peptide biosensor for Cu2+ metal ion quantification in coffee seeds.	
Ano de publicação: 	2024	
Fonte/Imprenta: 	Journal of Inorganic Biochemistry, v. 251, 112441, 2024.	
DOI: 	https://doi.org/10.1016/j.jinorgbio.2023.112441	
Idioma: 	Inglês	
Conteúdo: 	A prion-derived copper(II)-binding peptide was assembled onto a gold electrode for the building of a voltammetric biosensor for measuring the Cu2+ metal ion in biological samples. The chosen sequence was HCVNITKQHTVTTTT-NH2, with an appended cysteine residue for binding to the gold surface as a self-assembled monolayer and a histidine residue as the anchorage point for copper(II) complexation. The biosensor showed a linear range of 10-7 to 10-6 M with an 8.0 x 10-8 M detection limit and a 1.0 x 10-7 M quantification limit, with good precision, trueness, and absence of matrix effect. The quantification of Cu2+ was performed in the presence of other transition metal ions, such as Zn2+, Cd2+, Fe2+, or Ni2+, which indicates the excellent selectivity of the biosensor. When the modified electrode was applied for measuring copper(II) in calcined coffee seeds, a difference in copper amount was observed between two Coffea arabica cultivars that were submitted to a treatment with a copper-based antifungal, showing the applicability of the biosensor in the agricultural field.	
Palavras-Chave: 	Biossensor peptídico; Coffee seeds; Peptide biosensor; Prion peptide; Voltametria; Voltammetry.	
Thesagro: 	Café; Peptídeo; Semente.	
Categoria do assunto: 	F Plantas e Produtos de Origem Vegetal	
Registro original: 	Embrapa Unidades Centrais (AI-SEDE)	
Exemplares: 	CNPGL - (26257-1) - UPC - AP - DD -  -  -  - DIS
CENARGEN - (39358-1) - UPC - AP - DD -  -  -  - DIS
AI-SEDE - (67182-1) - UPC - AP - DD -  -  -  - DIS