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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
13/08/1996 |
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Data da última atualização: |
13/03/2008 |
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Autoria: |
SILVA, J. F. V. |
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Afiliação: |
EMBRAPA-CNPSo. Londrina,PR. |
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Título: |
Metodos usados para deteccao de cistos de Heterodera glycines, em amostras de sementes de soja. |
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Ano de publicação: |
1995 |
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Fonte/Imprenta: |
In: REUNIAO DE PESQUISA DE SOJA DA REGIAO CENTRAL DO BRASIL, 17., 1995, Goiania. Ata e resumos. Goiania: EMGOPA, 1995. |
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Páginas: |
p.163-164. |
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Série: |
(EMGOPA. Documentos, 28). |
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Idioma: |
Português |
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Palavras-Chave: |
Brasil; Nematoide de cisto; Parana; Seed; Soybean cyst nematode. |
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Thesagro: |
Heterodera Glycines; Semente; Soja. |
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Thesaurus Nal: |
Brazil. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00735naa a2200241 a 4500
001 1456190
005 2008-03-13
008 1995 bl uuuu u00u1 u #d
100 1 $aSILVA, J. F. V.
245 $aMetodos usados para deteccao de cistos de Heterodera glycines, em amostras de sementes de soja.
260 $c1995
300 $ap.163-164.
490 $a(EMGOPA. Documentos, 28).
650 $aBrazil
650 $aHeterodera Glycines
650 $aSemente
650 $aSoja
653 $aBrasil
653 $aNematoide de cisto
653 $aParana
653 $aSeed
653 $aSoybean cyst nematode
773 $tIn: REUNIAO DE PESQUISA DE SOJA DA REGIAO CENTRAL DO BRASIL, 17., 1995, Goiania. Ata e resumos. Goiania: EMGOPA, 1995.
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Registro original: |
Embrapa Soja (CNPSO) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
11/09/2017 |
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Data da última atualização: |
06/04/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
CAMPOS, C. G.; VERAS, H. C. T.; RIBEIRO, J. A. de A.; COSTA, P. P. K. G.; ARAÚJO, K. P.; RODRIGUES, C. M.; ALMEIDA, J. R. M. de; ABDELNUR, P. V. |
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Afiliação: |
CHRISTIANE GONÇALVES CAMPOS, Universidade Federal de Goiás; HENRIQUE CÉSAR TEIXEIRA VERAS, Universidade de Brasília; JOSE ANTONIO DE AQUINO RIBEIRO, CNPAE; PATRICIA PINTO KALIL G COSTA, CNPAE; KATIÚSCIA PEREIRA ARAÚJO; CLENILSON MARTINS RODRIGUES, CNPAE; JOAO RICARDO MOREIRA DE ALMEIDA, CNPAE; PATRICIA VERARDI ABDELNUR, CNPAE. |
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Título: |
New protocol based on UHPLC-MS/MS for quantitation of metabolites in xylose-fermenting yeasts. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Journal of the American Society for Mass Spectrometry, v. 28, n. 12, p. 2646-2657, 2017. |
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Páginas: |
p. 2646-2657 |
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DOI: |
10.1007/s13361-017-1786-9 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Xylose fermentation is a bottleneck in second-generation ethanol production. As such, a comprehensive understanding of xylose metabolism in naturally xylose-fermenting yeasts is essential for prospection and construction of recombinant yeast strains. The objective of the current study was to establish a reliable metabolomics protocol for quantification of key metabolites of xylose catabolism pathways in yeast, and to apply this protocol to Spathaspora arborariae. Ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) was used to quantify metabolites, and afterwards, sample preparation was optimized to examine yeast intracellular metabolites. S. arborariae was cultivated using xylose as a carbon source under aerobic and oxygen-limited conditions. Ion pair chromatography (IPC) and hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS) were shown to efficiently quantify 14 and 5 metabolites, respectively, in a more rapid chromatographic protocol than previously described. Thirteen and eleven metabolites were quantified in S. arborariae under aerobic and oxygen-limited conditions, respectively. This targeted metabolomics protocol is shown here to quantify a total of 19 metabolites, including sugars, phosphates, coenzymes, monosaccharides, and alcohols, from xylose catabolism pathways (glycolysis, pentose phosphate pathway, and tricarboxylic acid cycle) in yeast. Furthermore, to our knowledge, this is the first time that intracellular metabolites have been quantified in S. arborariae after xylose consumption. The results indicated that fine control of oxygen levels during fermentation is necessary to optimize ethanol production by S. arborariae. The protocol presented here may be applied to other yeast species and could support yeast genetic engineering to improve second generation ethanol production. Graphical Abstract ?. MenosAbstract: Xylose fermentation is a bottleneck in second-generation ethanol production. As such, a comprehensive understanding of xylose metabolism in naturally xylose-fermenting yeasts is essential for prospection and construction of recombinant yeast strains. The objective of the current study was to establish a reliable metabolomics protocol for quantification of key metabolites of xylose catabolism pathways in yeast, and to apply this protocol to Spathaspora arborariae. Ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) was used to quantify metabolites, and afterwards, sample preparation was optimized to examine yeast intracellular metabolites. S. arborariae was cultivated using xylose as a carbon source under aerobic and oxygen-limited conditions. Ion pair chromatography (IPC) and hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS) were shown to efficiently quantify 14 and 5 metabolites, respectively, in a more rapid chromatographic protocol than previously described. Thirteen and eleven metabolites were quantified in S. arborariae under aerobic and oxygen-limited conditions, respectively. This targeted metabolomics protocol is shown here to quantify a total of 19 metabolites, including sugars, phosphates, coenzymes, monosaccharides, and alcohols, from xylose catabolism pathways (glycolysis, pentose phosphate pathway, and tricarboxylic acid cycle) in yeast. Furthermore, to our knowledge, this is... Mostrar Tudo |
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Palavras-Chave: |
Spathaspora arborariae; UHPLC-MS/MS; Xylose fermentation. |
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Thesaurus NAL: |
Mass spectrometry; Metabolomics; Xylose. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/163703/1/Abdelnur-ASMassSoectiometry-2017.pdf
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Marc: |
LEADER 02860naa a2200301 a 4500
001 2075378
005 2021-04-06
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1007/s13361-017-1786-9$2DOI
100 1 $aCAMPOS, C. G.
245 $aNew protocol based on UHPLC-MS/MS for quantitation of metabolites in xylose-fermenting yeasts.$h[electronic resource]
260 $c2017
300 $ap. 2646-2657
520 $aAbstract: Xylose fermentation is a bottleneck in second-generation ethanol production. As such, a comprehensive understanding of xylose metabolism in naturally xylose-fermenting yeasts is essential for prospection and construction of recombinant yeast strains. The objective of the current study was to establish a reliable metabolomics protocol for quantification of key metabolites of xylose catabolism pathways in yeast, and to apply this protocol to Spathaspora arborariae. Ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) was used to quantify metabolites, and afterwards, sample preparation was optimized to examine yeast intracellular metabolites. S. arborariae was cultivated using xylose as a carbon source under aerobic and oxygen-limited conditions. Ion pair chromatography (IPC) and hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS) were shown to efficiently quantify 14 and 5 metabolites, respectively, in a more rapid chromatographic protocol than previously described. Thirteen and eleven metabolites were quantified in S. arborariae under aerobic and oxygen-limited conditions, respectively. This targeted metabolomics protocol is shown here to quantify a total of 19 metabolites, including sugars, phosphates, coenzymes, monosaccharides, and alcohols, from xylose catabolism pathways (glycolysis, pentose phosphate pathway, and tricarboxylic acid cycle) in yeast. Furthermore, to our knowledge, this is the first time that intracellular metabolites have been quantified in S. arborariae after xylose consumption. The results indicated that fine control of oxygen levels during fermentation is necessary to optimize ethanol production by S. arborariae. The protocol presented here may be applied to other yeast species and could support yeast genetic engineering to improve second generation ethanol production. Graphical Abstract ?.
650 $aMass spectrometry
650 $aMetabolomics
650 $aXylose
653 $aSpathaspora arborariae
653 $aUHPLC-MS/MS
653 $aXylose fermentation
700 1 $aVERAS, H. C. T.
700 1 $aRIBEIRO, J. A. de A.
700 1 $aCOSTA, P. P. K. G.
700 1 $aARAÚJO, K. P.
700 1 $aRODRIGUES, C. M.
700 1 $aALMEIDA, J. R. M. de
700 1 $aABDELNUR, P. V.
773 $tJournal of the American Society for Mass Spectrometry$gv. 28, n. 12, p. 2646-2657, 2017.
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Embrapa Agroenergia (CNPAE) |
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