Data/hora: 20/06/2024 03:22:56

Biblioteca(s): | Embrapa Soja.
Data corrente: | 01/10/2002
Data da última atualização: | 28/03/2024
Autoria: | SILVA, J. F. V.; FERRAZ, L. C. B. C.; ARIAS, C. A. A.; ABDELNOOR, R. V.
Afiliação: | CARLOS ALBERTO ARRABAL ARIAS, CNPSO; RICARDO VILELA ABDELNOOR, CNPSO.
Título: | Identificação de marcadores moleculares de microssatélites associados à resistência de genótipos de soja a Meloidogyne javanica.
Ano de publicação: | 2001
Fonte/Imprenta: | Nematologia Brasileira, Brasília, v. 25, n. 1, p. 79-83, 2001.
Idioma: | Português
Conteúdo: | Twenty loci of simple sequence repeats (SSR) were used to amplify the DNA of 120 F2 plants of the soyabean crosses BRS 133 (M. javanica-susceptible) x Coodetec 201 (resistant) and BRS 133 x PI 595099 (resistant). The loci are located close to some quantitative trait loci (QTLs) on the linkage group D1b+W and F. Each of the F2 (120) and F3 (120 families) generations and the parents (60 of each) were screened in a greenhouse for resistance to gall formation. The plants were grown in plastic tubes (4.5 cm in diameter x 19 cm length) filled with methyl bromide-fumigated soil amended with sand. The seedlings were individually inoculated with 3000 nematode eggs and evaluated by counting the root galls 30 days later. Analysis of variance indicated significant marker/nematode galls association for Satt 266 and Sat 133 (BRS133 x Coodetec 201) and HSP 176 (BRS 133 x PI 595099). This data corroborates the existence of QTLs conferring resistance to M. javanica located close to RFLP markers A725 on linkage-group D1b+W, and A806D and A186D/A757V on linkage-group F. However, the loci detected here did not explain much of the total phenotypic variation of the number of galls. Thus, further studies dealing with the evaluation of SSR loci at the same genomic location will be necessary until these markers can be fully applied to assisted selection of soyabean genotypes with resistance to M. javanica.
Thesagro: | Marcador Molecular; Meloidogyne Javanica; Resistência; Soja.
Thesaurus Nal: | Genetic resistance; Meloidogyne; Molecular genetics; Soybeans.
Categoria do assunto: | X Pesquisa, Tecnologia e Engenharia
Registro original: | Embrapa Soja (CNPSO)
Exemplares: | CNPSO - (19157-1) - UPC - AP - DD -  -  -  - DIS