| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Unidades Centrais. |
|
Data corrente: |
15/08/2018 |
|
Data da última atualização: |
25/02/2019 |
|
Autoria: |
CORRÊA, C. B.; VERAS, G. C.; SILVA, G. P. S. da; BRABO, M. F.; SILVA, E. M. |
|
Afiliação: |
Carline Barroso Corrêa, Universidade Federal do Pará - UFPA/Instituto de Estudos Costeiros/Faculdade de Engenharia de Pesca; Galileu Crovatto Veras, Universidade Federal do Pará - UFPA/Instituto de Estudos Costeiros/Faculdade de Engenharia de Pesca; Giselle Paula Silva da Silva, Universidade Federal do Pará - UFPA/Instituto de Estudos Costeiros/Faculdade de Engenharia de Pesca; Marcos Ferreira Brabo, Universidade Federal do Pará - UFPA/Instituto de Estudos Costeiros/Faculdade de Engenharia de Pesca; Evaldo Martins Silva, Universidade Federal do Pará - UFPA/Instituto de Estudos Costeiros/Faculdade de Engenharia de Pesca. |
|
Título: |
Performance, body composition, and physiological response of tambaqui fed diet with green tea extract. |
|
Ano de publicação: |
2018 |
|
Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 53, n. 5, p. 540-546, maio, 2018. |
|
Idioma: |
Inglês |
|
Notas: |
Titulo em português: Desempenho, composição corporal e respostas fisiológicas de tambaqui alimentados com dietas com extrato de chá-verde. |
|
Conteúdo: |
The objective of this work was to evaluate the performance, body composition, and physiological state of tambaqui (Colossoma macropomum) fingerlings fed diets containing different levels of green tea (Camellia sinensis) extract (GTE). A completely randomized design was used with five treatments, different levels of GTE (0.00, 0.05, 0.30, 0.55, and 0.80 g kg-1) and three replicates. The diets were offered to fingerlings twice a day, for 90 days. The increase of dietary GTE levels promoted a positive quadratic effect on body lipids, hepatic lipids, retention of body lipids, glucose, and total triglycerides, and the minimum values of these variables were estimated with 0.61, 0.54, 0.73, 0.60, and 0.30g kg-1 GTE in the diet, respectively. There was a negative quadratic effect of the extract on body protein and hemoglobin with the increased GTE dietary level, and the maximum values of these variables were estimated with 0.59 and 0.46 g kg-1 GTE dietary level, respectively. Total cholesterol levels showed a linear reduction with increased GTE dietary level. The inclusion of GTE in the diet, although not affecting performance, decreases body and hepatic fat; reduces the levels of total cholesterol, triglycerides, and glucose; and promotes the increase of body protein of tambaqui fingerlings, improving fish health conditions, during cultivation, and the quality of the final product. |
|
Palavras-Chave: |
Extrato de planta; Lipid reduction; Redução de lipídeo. |
|
Thesagro: |
Colossoma Macropomum; Fitoterapia; Flavonóide. |
|
Thesaurus Nal: |
Camellia sinensis; Flavonoids; Phytotherapy; Plant extracts. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/181466/1/Performance-body-composition-and-physiological.pdf
|
|
Marc: |
LEADER 02433naa a2200301 a 4500
001 2094139
005 2019-02-25
008 2018 bl uuuu u00u1 u #d
100 1 $aCORRÊA, C. B.
245 $aPerformance, body composition, and physiological response of tambaqui fed diet with green tea extract.$h[electronic resource]
260 $c2018
500 $aTitulo em português: Desempenho, composição corporal e respostas fisiológicas de tambaqui alimentados com dietas com extrato de chá-verde.
520 $aThe objective of this work was to evaluate the performance, body composition, and physiological state of tambaqui (Colossoma macropomum) fingerlings fed diets containing different levels of green tea (Camellia sinensis) extract (GTE). A completely randomized design was used with five treatments, different levels of GTE (0.00, 0.05, 0.30, 0.55, and 0.80 g kg-1) and three replicates. The diets were offered to fingerlings twice a day, for 90 days. The increase of dietary GTE levels promoted a positive quadratic effect on body lipids, hepatic lipids, retention of body lipids, glucose, and total triglycerides, and the minimum values of these variables were estimated with 0.61, 0.54, 0.73, 0.60, and 0.30g kg-1 GTE in the diet, respectively. There was a negative quadratic effect of the extract on body protein and hemoglobin with the increased GTE dietary level, and the maximum values of these variables were estimated with 0.59 and 0.46 g kg-1 GTE dietary level, respectively. Total cholesterol levels showed a linear reduction with increased GTE dietary level. The inclusion of GTE in the diet, although not affecting performance, decreases body and hepatic fat; reduces the levels of total cholesterol, triglycerides, and glucose; and promotes the increase of body protein of tambaqui fingerlings, improving fish health conditions, during cultivation, and the quality of the final product.
650 $aCamellia sinensis
650 $aFlavonoids
650 $aPhytotherapy
650 $aPlant extracts
650 $aColossoma Macropomum
650 $aFitoterapia
650 $aFlavonóide
653 $aExtrato de planta
653 $aLipid reduction
653 $aRedução de lipídeo
700 1 $aVERAS, G. C.
700 1 $aSILVA, G. P. S. da
700 1 $aBRABO, M. F.
700 1 $aSILVA, E. M.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 53, n. 5, p. 540-546, maio, 2018.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Agrobiologia. |
|
Data corrente: |
24/11/2016 |
|
Data da última atualização: |
23/10/2018 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 2 |
|
Autoria: |
SILVA, P. R. A. da; VIDAL, M. S.; SOARES, C. de P.; POLESE, V.; ARAUJO, J. L. S. de; BALDANI, J. I. |
|
Afiliação: |
PAULA RENATA ALVES DA SILVA, UFRRJ; MARCIA SOARES VIDAL, CNPAB; CLEITON DE PAULA SOARES, BOLSISTA DA EMBRAPA AGROBIOLOGIA; VALERIA POLESE, UFRRJ; JEAN LUIZ SIMOES DE ARAUJO, CNPAB; JOSE IVO BALDANI, CNPAB. |
|
Título: |
Selection and evaluation of reference genes for RT-qPCR expression studies on Burkholderia tropica strain Ppe8, a sugarcane-associated diazotrophic bacterium grown with different carbon sources or sugarcane juice |
|
Ano de publicação: |
2016 |
|
Fonte/Imprenta: |
Antonie van Leeuwenhoek, v. 109, n. 111, p. 1493-1502, 2016. |
|
DOI: |
10.1007/s10482-016-0751-0 |
|
Idioma: |
Inglês |
|
Notas: |
Epub, 17 Aug., 2016. |
|
Conteúdo: |
Among the members of the genus Burkholderia, Burkholderia tropica has the ability to fix nitrogen and promote sugarcane plant growth as well as act as a biological control agent. There is little information about how this bacterium metabolizes
carbohydrates as well as those carbon sources found in the sugarcane juice that accumulates in stems during plant growth. Reverse transcription quantitative PCR (RT-qPCR) can be used to evaluate changes in gene expression during bacterial growth on different carbon sources. Here we tested the expression of six reference genes, lpxC, gyrB, recA, rpoA, rpoB, and rpoD, when cells were grown with glucose, fructose, sucrose, mannitol, aconitic acid, and sugarcane juice as carbon sources. The lpxC, gyrB, and recA were selected as the most stable reference genes based on geNorm and NormFinder software analyses. Validation of these three reference genes during strain Ppe8 growth on the same carbon sources showed that genes involved in
glycogen biosynthesis (glgA, glgB, glgC) and trehalose biosynthesis (treY and treZ) were highly expressed when Ppe8 was grown in aconitic acid relative to other carbon sources, while otsA expression (trehalose biosynthesis) was reduced with all carbon
sources. In addition, the expression level of the ORF_6066 (gluconolactonase) gene was reduced on sugarcane juice. The results confirmed the stability of the three selected reference genes (lpxC, gyrB, and recA) during the RT-qPCR and also their robustness
by evaluating the relative expression of genes involved in glycogen and trehalose biosynthesis when strain Ppe8 was grown on different carbon sources and sugarcane juice. MenosAmong the members of the genus Burkholderia, Burkholderia tropica has the ability to fix nitrogen and promote sugarcane plant growth as well as act as a biological control agent. There is little information about how this bacterium metabolizes
carbohydrates as well as those carbon sources found in the sugarcane juice that accumulates in stems during plant growth. Reverse transcription quantitative PCR (RT-qPCR) can be used to evaluate changes in gene expression during bacterial growth on different carbon sources. Here we tested the expression of six reference genes, lpxC, gyrB, recA, rpoA, rpoB, and rpoD, when cells were grown with glucose, fructose, sucrose, mannitol, aconitic acid, and sugarcane juice as carbon sources. The lpxC, gyrB, and recA were selected as the most stable reference genes based on geNorm and NormFinder software analyses. Validation of these three reference genes during strain Ppe8 growth on the same carbon sources showed that genes involved in
glycogen biosynthesis (glgA, glgB, glgC) and trehalose biosynthesis (treY and treZ) were highly expressed when Ppe8 was grown in aconitic acid relative to other carbon sources, while otsA expression (trehalose biosynthesis) was reduced with all carbon
sources. In addition, the expression level of the ORF_6066 (gluconolactonase) gene was reduced on sugarcane juice. The results confirmed the stability of the three selected reference genes (lpxC, gyrB, and recA) during the RT-qPCR and also their robustness
by evalua... Mostrar Tudo |
|
Palavras-Chave: |
Carbon metabolism; Diazotrophic bacteria; Normalizing genes; Real time PCR; Relative expression. |
|
Categoria do assunto: |
S Ciências Biológicas |
|
Marc: |
LEADER 02573naa a2200265 a 4500
001 2057134
005 2018-10-23
008 2016 bl --- 0-- u #d
024 7 $a10.1007/s10482-016-0751-0$2DOI
100 1 $aSILVA, P. R. A. da
245 $aSelection and evaluation of reference genes for RT-qPCR expression studies on Burkholderia tropica strain Ppe8, a sugarcane-associated diazotrophic bacterium grown with different carbon sources or sugarcane juice
260 $c2016
500 $aEpub, 17 Aug., 2016.
520 $aAmong the members of the genus Burkholderia, Burkholderia tropica has the ability to fix nitrogen and promote sugarcane plant growth as well as act as a biological control agent. There is little information about how this bacterium metabolizes carbohydrates as well as those carbon sources found in the sugarcane juice that accumulates in stems during plant growth. Reverse transcription quantitative PCR (RT-qPCR) can be used to evaluate changes in gene expression during bacterial growth on different carbon sources. Here we tested the expression of six reference genes, lpxC, gyrB, recA, rpoA, rpoB, and rpoD, when cells were grown with glucose, fructose, sucrose, mannitol, aconitic acid, and sugarcane juice as carbon sources. The lpxC, gyrB, and recA were selected as the most stable reference genes based on geNorm and NormFinder software analyses. Validation of these three reference genes during strain Ppe8 growth on the same carbon sources showed that genes involved in glycogen biosynthesis (glgA, glgB, glgC) and trehalose biosynthesis (treY and treZ) were highly expressed when Ppe8 was grown in aconitic acid relative to other carbon sources, while otsA expression (trehalose biosynthesis) was reduced with all carbon sources. In addition, the expression level of the ORF_6066 (gluconolactonase) gene was reduced on sugarcane juice. The results confirmed the stability of the three selected reference genes (lpxC, gyrB, and recA) during the RT-qPCR and also their robustness by evaluating the relative expression of genes involved in glycogen and trehalose biosynthesis when strain Ppe8 was grown on different carbon sources and sugarcane juice.
653 $aCarbon metabolism
653 $aDiazotrophic bacteria
653 $aNormalizing genes
653 $aReal time PCR
653 $aRelative expression
700 1 $aVIDAL, M. S.
700 1 $aSOARES, C. de P.
700 1 $aPOLESE, V.
700 1 $aARAUJO, J. L. S. de
700 1 $aBALDANI, J. I.
773 $tAntonie van Leeuwenhoek$gv. 109, n. 111, p. 1493-1502, 2016.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Agrobiologia (CNPAB) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
