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Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
12/01/2011 |
Data da última atualização: |
01/07/2016 |
Autoria: |
ARAÚJO, V. R.; SILVA, C. M. G. da; MAGALHÃES, D. M.; SILVA, G. M. da; BAÓ, S. N.; SILVA, J. R. V.; FIGUEIREDO, J. R. de; RODRIGUES, A. P. R. |
Título: |
Effect of bone morphogenetic protein-7 (BMP-7) on in vitro survival of caprine preantral follicles. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 30, n. 4, p. 305-310, abr., 2010. |
Idioma: |
Inglês |
Conteúdo: |
This study was conducted in order to verify the effect of different concentrations of BMP-7 in the in vitro survival and development of caprine preantral follicles. Fragments of caprine ovarian cortical tissue were cultured for 1 or 7 days in Minimum Essential Medium (MEM+) supplemented with different concentrations of BMP-7 (1, 10, 50 or 100ng/ml). Non-cultured fragments or those cultured for 1 or 7 days were processed for classical histology and transmission electron microscopy (TEM). Parameters such as follicular survival, activation and growth were evaluated. The results showed that, after 1 or 7 days of culture, the percentage of morphologically normal follicles was significantly reduced in all treatments when compared with fresh control, except at 1ng/ml of BMP-7 for 1 day. In addition, the concentration of 10ng/ml of BMP-7 significantly increases follicular diameter from day 1 to 7 of culture. There was no influence of the other concentrations of BMP-7 regarding to the follicular and oocyte diameter. Ultrastructure studies confirmed follicular integrity after 7 days of culture in 1ng/ml BMP-7. In conclusion, small concentrations of BMP-7 can improve the survival and growth of caprine preantral follicles during in vitro culture.(AU).
[Efeito da proteína morfogenética óssea 7 (BMP-7) para a sobrevivência in vitro de folículos pré-antrais caprinos].
Resumo: O presente trabalho foi conduzido de modo a se verificar o efeito de diferentes concentrações da BMP-7 no desenvolvimento in vitro de folículos pré-antrais caprinos. Fragmentos de tecido cortical ovariano caprino foram cultivados por 1 ou 7 dias em Minimum Essential Medium (MEM+) suplementado com diferentes concentrações de BMP-7 (1, 10, 50 ou 100ng/ml). Os fragmentos não cultivados ou aqueles cultivados por 1 ou 7 dias foram processados para histologia clássica e microscopia eletrônica de transmissão (TEM), sendo avaliados parâmetros morfológicos indicativos de viabilidade, ativação e crescimento. Os resultados mostraram que o percentual de folículos morfologicamente normais diminuiu significativamente em todos os tratamentos quando comparados ao controle, exceto na concentração de 1ng/ml por 1 dia de cultivo. Já no D7 todos os tratamentos reduziram significativamente os percentuais de folículos morfologicamente normais. Utilizando 10ng/ml de BMP-7 foi observado um aumento significativo no diâmetro folicular quando comparados os diferentes períodos de cultivo. Não houve influência das demais concentrações de BMP-7 quando avaliados além do diâmetro folicular o diâmetro oocitário. A análise por TEM confirmou a integridade ultra-estrutural nos folículos após 7 dias de cultivo com 1ng/ml de BMP-7 . Em conclusão, o BMP-7 em baixas concentrações pode melhorar a sobrevivência e o crescimento durante o cultivo in vitro de folículos pré-antrais caprinos. MenosThis study was conducted in order to verify the effect of different concentrations of BMP-7 in the in vitro survival and development of caprine preantral follicles. Fragments of caprine ovarian cortical tissue were cultured for 1 or 7 days in Minimum Essential Medium (MEM+) supplemented with different concentrations of BMP-7 (1, 10, 50 or 100ng/ml). Non-cultured fragments or those cultured for 1 or 7 days were processed for classical histology and transmission electron microscopy (TEM). Parameters such as follicular survival, activation and growth were evaluated. The results showed that, after 1 or 7 days of culture, the percentage of morphologically normal follicles was significantly reduced in all treatments when compared with fresh control, except at 1ng/ml of BMP-7 for 1 day. In addition, the concentration of 10ng/ml of BMP-7 significantly increases follicular diameter from day 1 to 7 of culture. There was no influence of the other concentrations of BMP-7 regarding to the follicular and oocyte diameter. Ultrastructure studies confirmed follicular integrity after 7 days of culture in 1ng/ml BMP-7. In conclusion, small concentrations of BMP-7 can improve the survival and growth of caprine preantral follicles during in vitro culture.(AU).
[Efeito da proteína morfogenética óssea 7 (BMP-7) para a sobrevivência in vitro de folículos pré-antrais caprinos].
Resumo: O presente trabalho foi conduzido de modo a se verificar o efeito de diferentes concentrações da BMP-7 no desenvol... Mostrar Tudo |
Palavras-Chave: |
BMP-7; Foliculo pre-antral; Foliculogênese; Proteína morfogenética óssea. |
Thesagro: |
Caprino; Fator de Crescimento; Ovário. |
Categoria do assunto: |
-- |
Marc: |
LEADER 03766naa a2200289 a 4500 001 1872852 005 2016-07-01 008 2010 bl uuuu u00u1 u #d 100 1 $aARAÚJO, V. R. 245 $aEffect of bone morphogenetic protein-7 (BMP-7) on in vitro survival of caprine preantral follicles. 260 $c2010 520 $aThis study was conducted in order to verify the effect of different concentrations of BMP-7 in the in vitro survival and development of caprine preantral follicles. Fragments of caprine ovarian cortical tissue were cultured for 1 or 7 days in Minimum Essential Medium (MEM+) supplemented with different concentrations of BMP-7 (1, 10, 50 or 100ng/ml). Non-cultured fragments or those cultured for 1 or 7 days were processed for classical histology and transmission electron microscopy (TEM). Parameters such as follicular survival, activation and growth were evaluated. The results showed that, after 1 or 7 days of culture, the percentage of morphologically normal follicles was significantly reduced in all treatments when compared with fresh control, except at 1ng/ml of BMP-7 for 1 day. In addition, the concentration of 10ng/ml of BMP-7 significantly increases follicular diameter from day 1 to 7 of culture. There was no influence of the other concentrations of BMP-7 regarding to the follicular and oocyte diameter. Ultrastructure studies confirmed follicular integrity after 7 days of culture in 1ng/ml BMP-7. In conclusion, small concentrations of BMP-7 can improve the survival and growth of caprine preantral follicles during in vitro culture.(AU). [Efeito da proteína morfogenética óssea 7 (BMP-7) para a sobrevivência in vitro de folículos pré-antrais caprinos]. Resumo: O presente trabalho foi conduzido de modo a se verificar o efeito de diferentes concentrações da BMP-7 no desenvolvimento in vitro de folículos pré-antrais caprinos. Fragmentos de tecido cortical ovariano caprino foram cultivados por 1 ou 7 dias em Minimum Essential Medium (MEM+) suplementado com diferentes concentrações de BMP-7 (1, 10, 50 ou 100ng/ml). Os fragmentos não cultivados ou aqueles cultivados por 1 ou 7 dias foram processados para histologia clássica e microscopia eletrônica de transmissão (TEM), sendo avaliados parâmetros morfológicos indicativos de viabilidade, ativação e crescimento. Os resultados mostraram que o percentual de folículos morfologicamente normais diminuiu significativamente em todos os tratamentos quando comparados ao controle, exceto na concentração de 1ng/ml por 1 dia de cultivo. Já no D7 todos os tratamentos reduziram significativamente os percentuais de folículos morfologicamente normais. Utilizando 10ng/ml de BMP-7 foi observado um aumento significativo no diâmetro folicular quando comparados os diferentes períodos de cultivo. Não houve influência das demais concentrações de BMP-7 quando avaliados além do diâmetro folicular o diâmetro oocitário. A análise por TEM confirmou a integridade ultra-estrutural nos folículos após 7 dias de cultivo com 1ng/ml de BMP-7 . Em conclusão, o BMP-7 em baixas concentrações pode melhorar a sobrevivência e o crescimento durante o cultivo in vitro de folículos pré-antrais caprinos. 650 $aCaprino 650 $aFator de Crescimento 650 $aOvário 653 $aBMP-7 653 $aFoliculo pre-antral 653 $aFoliculogênese 653 $aProteína morfogenética óssea 700 1 $aSILVA, C. M. G. da 700 1 $aMAGALHÃES, D. M. 700 1 $aSILVA, G. M. da 700 1 $aBAÓ, S. N. 700 1 $aSILVA, J. R. V. 700 1 $aFIGUEIREDO, J. R. de 700 1 $aRODRIGUES, A. P. R. 773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 30, n. 4, p. 305-310, abr., 2010.
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Registro Completo
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
08/06/2004 |
Data da última atualização: |
06/05/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
BRITO, L. F. C.; SILVA, A. E. D. F. D.; BARBOSA, R. T.; KASTELIC, J. P. |
Afiliação: |
LEONARDO F. C. BRITO, UNESP; ANTONIO EMIDIO DIAS F DA SILVA, Cenargen; ROGERIO TAVEIRA BARBOSA, CPPSE; JOHN P. KASTELIC, Agriculture and Agri-Food Canada. |
Título: |
Testicular thermoregulation in Bos indicus, crossbred and Bos taurus bulls: relationship with scrotal, testicular morphology, and effects on semen quality and sperm production. |
Ano de publicação: |
2004 |
Fonte/Imprenta: |
Theriogenology, v.61, n.2-3, p.511-528, jan. 2004. |
DOI: |
https://doi.org/10.1016/S0093-691X(03)00231-0 |
Idioma: |
Inglês |
Conteúdo: |
Mechanisms of testicular thermoregulation, the relationship of scrotal, testicular vascular cone (TVC), and testicular morphology with thermoregulatory capability, and their effects on semen quality and sperm production were studied in 20 Bos indicus, 28 crossbred, and 26 Bos taurus bulls. The ratio of testicular artery length and volume to testicular volume were larger (P<0.05) in B. indicus and crossbred bulls than in B. taurus bulls (1.03 and 0.94 cm/cm3 versus 0.48 cm/cm3; 0.034 and 0.047 ml/cm3 versus 0.017 ml/cm3, respectively). Testicular artery wall thickness (average 192.5, 229.0, and 290.0 ?m, respectively) and arterial?venous blood distance in the TVC (average 330.5, 373.7, and 609.4 ?m, respectively) were smallest in B. indicus, intermediary in crossbred, and greatest in B. taurus bulls (P<0.05); the proximity between arterial and venous blood was consistent with the estimated decrease in arterial blood temperature after passage through the TVC (5.9, 5.0, and 2.9 °C, in B. indicus, crossbred, and B. taurus bulls, respectively). In crossbred and B. taurus bulls, there was a positive top-to-bottom scrotal temperature gradient and a negative testicular subtunic temperature gradient. However, in B. indicus bulls, both scrotal and testicular subtunic temperatures gradients were positive. Differences in the vascular arrangement, characteristics of the artery (e.g. wall thickness) or thickness of the tunica albuginea may have affected the testicular arterial blood and subtunic temperatures in B. indicus bulls. Better testicular thermoregulatory capability was associated with increased scrotal shape (pendulosity), testicular artery length and volume, and top-to-bottom gradient of the distance between the artery wall and the veins in the TVC. Increased semen quality was associated with increased testicular volume and scrotal subcutaneous (SQT) temperature gradient, and with decreased scrotal surface and testicular temperatures. Increased sperm production was associated with increased testicular artery volume, testicular volume, and SQT temperature gradient, and with decreased testicular artery wall thickness, scrotal circumference (SC), and scrotal surface, testicular subtunic, and epididymal temperatures. In conclusion, morphology of the TVC may contribute to the greater resistance of B. indicus bulls to high ambient temperatures by conferring a better testicular blood supply and by facilitating heat transfer between the testicular artery and veins. Testicular thermoregulation was associated with opposing scrotal and testicular subtunic temperatures gradients only in crossbred and B. taurus bulls. Scrotal, TVC, and testicular morphology influence testicular thermoregulatory capability and were associated with differences in semen quality and sperm production. MenosMechanisms of testicular thermoregulation, the relationship of scrotal, testicular vascular cone (TVC), and testicular morphology with thermoregulatory capability, and their effects on semen quality and sperm production were studied in 20 Bos indicus, 28 crossbred, and 26 Bos taurus bulls. The ratio of testicular artery length and volume to testicular volume were larger (P<0.05) in B. indicus and crossbred bulls than in B. taurus bulls (1.03 and 0.94 cm/cm3 versus 0.48 cm/cm3; 0.034 and 0.047 ml/cm3 versus 0.017 ml/cm3, respectively). Testicular artery wall thickness (average 192.5, 229.0, and 290.0 ?m, respectively) and arterial?venous blood distance in the TVC (average 330.5, 373.7, and 609.4 ?m, respectively) were smallest in B. indicus, intermediary in crossbred, and greatest in B. taurus bulls (P<0.05); the proximity between arterial and venous blood was consistent with the estimated decrease in arterial blood temperature after passage through the TVC (5.9, 5.0, and 2.9 °C, in B. indicus, crossbred, and B. taurus bulls, respectively). In crossbred and B. taurus bulls, there was a positive top-to-bottom scrotal temperature gradient and a negative testicular subtunic temperature gradient. However, in B. indicus bulls, both scrotal and testicular subtunic temperatures gradients were positive. Differences in the vascular arrangement, characteristics of the artery (e.g. wall thickness) or thickness of the tunica albuginea may have affected the testicular arterial blood and s... Mostrar Tudo |
Palavras-Chave: |
Circuferênica escrotal; Semem. |
Thesagro: |
Bos Indicus; Bos Taurus; Qualidade. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 03604naa a2200229 a 4500 001 1046842 005 2022-05-06 008 2004 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/S0093-691X(03)00231-0$2DOI 100 1 $aBRITO, L. F. C. 245 $aTesticular thermoregulation in Bos indicus, crossbred and Bos taurus bulls$brelationship with scrotal, testicular morphology, and effects on semen quality and sperm production.$h[electronic resource] 260 $c2004 520 $aMechanisms of testicular thermoregulation, the relationship of scrotal, testicular vascular cone (TVC), and testicular morphology with thermoregulatory capability, and their effects on semen quality and sperm production were studied in 20 Bos indicus, 28 crossbred, and 26 Bos taurus bulls. The ratio of testicular artery length and volume to testicular volume were larger (P<0.05) in B. indicus and crossbred bulls than in B. taurus bulls (1.03 and 0.94 cm/cm3 versus 0.48 cm/cm3; 0.034 and 0.047 ml/cm3 versus 0.017 ml/cm3, respectively). Testicular artery wall thickness (average 192.5, 229.0, and 290.0 ?m, respectively) and arterial?venous blood distance in the TVC (average 330.5, 373.7, and 609.4 ?m, respectively) were smallest in B. indicus, intermediary in crossbred, and greatest in B. taurus bulls (P<0.05); the proximity between arterial and venous blood was consistent with the estimated decrease in arterial blood temperature after passage through the TVC (5.9, 5.0, and 2.9 °C, in B. indicus, crossbred, and B. taurus bulls, respectively). In crossbred and B. taurus bulls, there was a positive top-to-bottom scrotal temperature gradient and a negative testicular subtunic temperature gradient. However, in B. indicus bulls, both scrotal and testicular subtunic temperatures gradients were positive. Differences in the vascular arrangement, characteristics of the artery (e.g. wall thickness) or thickness of the tunica albuginea may have affected the testicular arterial blood and subtunic temperatures in B. indicus bulls. Better testicular thermoregulatory capability was associated with increased scrotal shape (pendulosity), testicular artery length and volume, and top-to-bottom gradient of the distance between the artery wall and the veins in the TVC. Increased semen quality was associated with increased testicular volume and scrotal subcutaneous (SQT) temperature gradient, and with decreased scrotal surface and testicular temperatures. Increased sperm production was associated with increased testicular artery volume, testicular volume, and SQT temperature gradient, and with decreased testicular artery wall thickness, scrotal circumference (SC), and scrotal surface, testicular subtunic, and epididymal temperatures. In conclusion, morphology of the TVC may contribute to the greater resistance of B. indicus bulls to high ambient temperatures by conferring a better testicular blood supply and by facilitating heat transfer between the testicular artery and veins. Testicular thermoregulation was associated with opposing scrotal and testicular subtunic temperatures gradients only in crossbred and B. taurus bulls. Scrotal, TVC, and testicular morphology influence testicular thermoregulatory capability and were associated with differences in semen quality and sperm production. 650 $aBos Indicus 650 $aBos Taurus 650 $aQualidade 653 $aCircuferênica escrotal 653 $aSemem 700 1 $aSILVA, A. E. D. F. D. 700 1 $aBARBOSA, R. T. 700 1 $aKASTELIC, J. P. 773 $tTheriogenology$gv.61, n.2-3, p.511-528, jan. 2004.
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