Data/hora: 22/06/2024 20:51:31

Biblioteca(s): | Embrapa Meio Ambiente.
Data corrente: | 05/04/2021
Data da última atualização: | 05/04/2021
Autoria: | MEDEIROS, A. M. Z.; KHAN, L. U.; SILVA, G. H. da; OSPINA, C. A.; ALVES, O. L.; CASTRO, V. L. S. S. de; MARTINEZ, D. S. T.
Afiliação: | ALINE MARIA ZIGIOTTO DE MEDEIROS, CNPEM; LATIF ULLAH KHAN, CNPEM; GABRIELA HELENA DA SILVA, CNPEM; CARLOS ALBERTO OSPINA, CNPEM; OSWALDO LUIZ ALVES, IQ-UNICAMP; VERA LUCIA SCHERHOLZ S DE CASTRO, CNPMA; DIEGO STÉFANI TEODORO MARTINEZ, CNPEM.
Título: | Graphene oxide-silver nanoparticle hybrid material: an integrated nanosafety study in zebrafish embryos.
Ano de publicação: | 2021
Fonte/Imprenta: | Ecotoxicology and Environmental Safety, v. 209, article 111776, 2021.
ISSN: | 0147-6513
DOI: | https://doi.org/10.1016/j.ecoenv.2020.111776
Idioma: | Inglês
Conteúdo: | Abstract: This work reports an integrated nanosafety study including the synthesis and characterization of the graphene oxide-silver nanoparticle hybrid material (GO-AgNPs) and its nano-ecotoxicity evaluation in the zebrafish embryo model. The influences of natural organic matter (NOM) and a chorion embryo membrane were considered in this study, looking towards more environmentally realistic scenarios and standardized nanotoxicity testing. The nanohybrid was successfully synthesized using the NaBH4 aqueous method, and AgNPs (~ 5.8 nm) were evenly distributed over the GO surface. GO-AgNPs showed a dose-response acute toxicity: the LC50 was 1.5 mg L-1 for chorionated embryos. The removal of chorion, however, increased this toxic effect by 50%. Furthermore, the presence of NOM mitigated mortality, and LC50 for GO-AgNPs changed respectively from 2.3 to 1.2 mg L-1 for chorionated and de-chorionated embryos. Raman spectroscopy confirmed the ingestion of GO by embryos; but without displaying acute toxicity up to 100 mg L-1, indicating that the silver drove toxicity down. Additionally, it was observed that silver nanoparticle dissolution has a minimal effect on these observed toxicity results. Finally, understanding the influence of chorion membranes and NOM is a critical step towards the standardization of testing for zebrafish embryo toxicity in safety assessments and regulatory issues.
Palavras-Chave: | Alternative methods; Grafeno; Nanoecotoxicology; Nanohybrids; Nanomateriais; Nanotoxicity.
Thesagro: | Composto de Carbono; Peixe de Água Doce; Toxidez.
Thesaurus Nal: | chorion; Danio rerio; Ecotoxicology; Graphene oxide; Nanomaterials.
Categoria do assunto: | P Recursos Naturais, Ciências Ambientais e da Terra
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/222338/1/Castro-Graphene-oxide-2021.pdf
Registro original: | Embrapa Meio Ambiente (CNPMA)
Exemplares: | CNPMA - (16956-1) - UPC - AP - DD -  -  -  - DIS