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Registro Completo |
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Biblioteca(s): |
Embrapa Rondônia. |
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Data corrente: |
18/11/2021 |
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Data da última atualização: |
19/05/2022 |
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Tipo da produção científica: |
Boletim de Pesquisa e Desenvolvimento |
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Autoria: |
DIAS, J. A.; OLIVEIRA, A. M. de; MACEDO, S. C. C.; FARIA, G. V.; CONSTANTINO, N. A. S.; SILVA, F. de A. C. |
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Afiliação: |
JULIANA ALVES DIAS, CPAF-RO; AUDENICE MIRANDA DE OLIVEIRA; STEFANY CRISTINA CORDEIRO MACEDO; GUILHERME VIEIRA FARIA, CPAF-RO; NAÍLE ÁGATA SOUZA CONSTANTINO; FRANCISCO DE ASSIS CORREA SILVA, CPAF-RO. |
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Título: |
Microrganismos deteriorantes do leite armazenado em tanques de resfriamento coletivo em Rondônia. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Porto Velho: Embrapa Rondônia, 2021. |
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Páginas: |
27 p. |
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Série: |
(Embrapa Rondônia. Boletim de pesquisa e desenvolvimento, 83). |
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ISSN: |
1677-8618 |
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Idioma: |
Português |
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Conteúdo: |
Considerando a predominância do uso de tanques de resfriamento coletivos no armazenamento do leite cru em Rondônia e a grande diversidade de microrganismos deletérios ao leite, o objetivo do trabalho foi avaliar a microbiota deteriorante do leite armazenado em 23 tanques coletivos da microrregião de Ji-Paraná e Cacoal. Para a contagem dos microrganismos utilizou-se semeadura em duplicata de 1 mL em placas de Petrifilm® AC aeróbios mesófilos e Petrifilm® EC coliformes totais de acordo com o fabricante. Para a contagem de psicrotróficos e termodúricos psicotróficos, as amostras foram semeadas em ágar padrão leite a 10%. Para a pesquisa de microrganismos termodúricos mesófilos e psicrotróficos, as amostras foram submetidas ao tratamento térmico (62,8 ± 0,5 °C) e resfriada a 10 ºC. A determinação da Contagem Total de Bactérias, convertidas em Contagem Padrão em Placas (CPP), e Contagem de Células Somáticas (CCS) foi realizada pelo método de citometria de fluxo em equipamento automatizado. A mediana de produtores dos tanques avaliados foi de 5 variando de 2 a 19. Dos 23 tanques avaliados, 65,2% presentaram CPP acima de 300.000 UFC/mL, limite em vigor definido na legislação. A mediana dos resultados de CPP foi de 415.000 UFC/mL para as amostras de tanque da microrregião de Ji-Paraná e 555.000 UFC/mL para tanques da microrregião de Cacoal, não sendo observada diferença estatística da CPP entre as regiões. A mediana da contagem de mesófilos, psicrotróficos, psicrotróficos proteolíticos, coliformes, termodúricos mesófilos e psicrotróficos foram, respectivamente: 2,8 x 105 UFC/mL, 6,8 x 105 UFC/mL, 4,6 x 105 UFC/mL, 2,0 x 104 UFC/mL, 1,5 x 103 UFC/mL, 1,3 x 103 UFC/mL. O resultado deste trabalho demonstrou altas contagens de bactérias deteriorantes no leite cru resfriado armazenado em tanques coletivos, indicando a ocorrência de efeitos deletérios ao leite e derivados lácteos produzidos, e de prejuízos econômicos e tecnológicos para a indústria regional. A definição de estratégias específicas, priorizando a adoção de boas práticas de ordenha e adequação da logística, estrutura e processos para o resfriamento do leite, é fundamental para a redução da contaminação microbiológica da matéria-prima e adequação à legislação. Considering the predominance of using collective cooling tanks for the storage of raw milk in Rondônia and the great diversity of microorganisms harmful to milk, the objective of the work was to evaluate the deteriorating microbiota of milk stored in 23 collective tanks in the micro-region of Ji- Paraná and Cacoal. For counting the microorganisms, seeding in duplicate of 1 mL was used in aerobic mesophilic Petrifilm® AC and total coliform Petrifilm® EC plates according to the manufacturer. For counting psychrotrophic and psychotrophic thermoduric, the samples were seeded on standard 10% milk agar. For the research of psychrotrophic and thermoduric mesophilic microorganisms, the samples were submitted to heat treatment (62.8 ± 0.5 °C) and cooled to 10 ºC. The determination of the Total Bacteria Count, converted to Standard Plate Count (SPC), and Somatic Cell Count (SCC) were performed by the flow cytometry method in automated equipment. The median of farmers of the tanks evaluated was 5, varying from 2 to 19. Of the 23 tanks evaluated, 65.2% had SPC above 300,000 CFU/mL, the current limit defined in legislation. The median of SPC results was 415,000 CFU/mL for tank samples from the Ji-Paraná micro-region and 555,000 CFU/mL for tanks in the Cacoal micro-region, with no statistical difference in SPC between regions. The median count of mesophilic, psychrotrophic, proteolytic psychrotrophic, coliform, mesophilic and psychrotrophic thermoduric were 2.8x105 CFU/mL, 6.8x105 CFU/mL, 4.6x105 CFU/mL, 2.0x104 CFU/mL, 1,5x103 CFU/mL and 1.3 x103 CFU/mL, respectively. The result of this work revealed high deteriorating bacteria counts in raw milk stored in collective tanks, indicating the occurrence of deleterious effects to the milk and dairy products, and economic and technological losses for the regional industry. Defining specific strategies, prioritizing in the adoption of good practices in milking and adaptations in logistic, structure and processes for the milk cooling is fundamental for the microbiological contamination reduction in raw material and adequacy to legislation. MenosConsiderando a predominância do uso de tanques de resfriamento coletivos no armazenamento do leite cru em Rondônia e a grande diversidade de microrganismos deletérios ao leite, o objetivo do trabalho foi avaliar a microbiota deteriorante do leite armazenado em 23 tanques coletivos da microrregião de Ji-Paraná e Cacoal. Para a contagem dos microrganismos utilizou-se semeadura em duplicata de 1 mL em placas de Petrifilm® AC aeróbios mesófilos e Petrifilm® EC coliformes totais de acordo com o fabricante. Para a contagem de psicrotróficos e termodúricos psicotróficos, as amostras foram semeadas em ágar padrão leite a 10%. Para a pesquisa de microrganismos termodúricos mesófilos e psicrotróficos, as amostras foram submetidas ao tratamento térmico (62,8 ± 0,5 °C) e resfriada a 10 ºC. A determinação da Contagem Total de Bactérias, convertidas em Contagem Padrão em Placas (CPP), e Contagem de Células Somáticas (CCS) foi realizada pelo método de citometria de fluxo em equipamento automatizado. A mediana de produtores dos tanques avaliados foi de 5 variando de 2 a 19. Dos 23 tanques avaliados, 65,2% presentaram CPP acima de 300.000 UFC/mL, limite em vigor definido na legislação. A mediana dos resultados de CPP foi de 415.000 UFC/mL para as amostras de tanque da microrregião de Ji-Paraná e 555.000 UFC/mL para tanques da microrregião de Cacoal, não sendo observada diferença estatística da CPP entre as regiões. A mediana da contagem de mesófilos, psicrotróficos, psicrotróficos proteolíti... Mostrar Tudo |
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Palavras-Chave: |
Cooling tank; Microbiota. |
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Thesagro: |
Armazenamento; Leite; Qualidade; Resfriamento; Tanque. |
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Thesaurus Nal: |
Cold storage; Microorganisms; Milk quality. |
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Categoria do assunto: |
Q Alimentos e Nutrição Humana |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/229765/1/cpafro-18620.pdf
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Marc: |
LEADER 05367nam a2200325 a 4500
001 2136312
005 2022-05-19
008 2021 bl uuuu u0uu1 u #d
022 $a1677-8618
100 1 $aDIAS, J. A.
245 $aMicrorganismos deteriorantes do leite armazenado em tanques de resfriamento coletivo em Rondônia.$h[electronic resource]
260 $aPorto Velho: Embrapa Rondônia$c2021
300 $a27 p.
490 $a(Embrapa Rondônia. Boletim de pesquisa e desenvolvimento, 83).
520 $aConsiderando a predominância do uso de tanques de resfriamento coletivos no armazenamento do leite cru em Rondônia e a grande diversidade de microrganismos deletérios ao leite, o objetivo do trabalho foi avaliar a microbiota deteriorante do leite armazenado em 23 tanques coletivos da microrregião de Ji-Paraná e Cacoal. Para a contagem dos microrganismos utilizou-se semeadura em duplicata de 1 mL em placas de Petrifilm® AC aeróbios mesófilos e Petrifilm® EC coliformes totais de acordo com o fabricante. Para a contagem de psicrotróficos e termodúricos psicotróficos, as amostras foram semeadas em ágar padrão leite a 10%. Para a pesquisa de microrganismos termodúricos mesófilos e psicrotróficos, as amostras foram submetidas ao tratamento térmico (62,8 ± 0,5 °C) e resfriada a 10 ºC. A determinação da Contagem Total de Bactérias, convertidas em Contagem Padrão em Placas (CPP), e Contagem de Células Somáticas (CCS) foi realizada pelo método de citometria de fluxo em equipamento automatizado. A mediana de produtores dos tanques avaliados foi de 5 variando de 2 a 19. Dos 23 tanques avaliados, 65,2% presentaram CPP acima de 300.000 UFC/mL, limite em vigor definido na legislação. A mediana dos resultados de CPP foi de 415.000 UFC/mL para as amostras de tanque da microrregião de Ji-Paraná e 555.000 UFC/mL para tanques da microrregião de Cacoal, não sendo observada diferença estatística da CPP entre as regiões. A mediana da contagem de mesófilos, psicrotróficos, psicrotróficos proteolíticos, coliformes, termodúricos mesófilos e psicrotróficos foram, respectivamente: 2,8 x 105 UFC/mL, 6,8 x 105 UFC/mL, 4,6 x 105 UFC/mL, 2,0 x 104 UFC/mL, 1,5 x 103 UFC/mL, 1,3 x 103 UFC/mL. O resultado deste trabalho demonstrou altas contagens de bactérias deteriorantes no leite cru resfriado armazenado em tanques coletivos, indicando a ocorrência de efeitos deletérios ao leite e derivados lácteos produzidos, e de prejuízos econômicos e tecnológicos para a indústria regional. A definição de estratégias específicas, priorizando a adoção de boas práticas de ordenha e adequação da logística, estrutura e processos para o resfriamento do leite, é fundamental para a redução da contaminação microbiológica da matéria-prima e adequação à legislação. Considering the predominance of using collective cooling tanks for the storage of raw milk in Rondônia and the great diversity of microorganisms harmful to milk, the objective of the work was to evaluate the deteriorating microbiota of milk stored in 23 collective tanks in the micro-region of Ji- Paraná and Cacoal. For counting the microorganisms, seeding in duplicate of 1 mL was used in aerobic mesophilic Petrifilm® AC and total coliform Petrifilm® EC plates according to the manufacturer. For counting psychrotrophic and psychotrophic thermoduric, the samples were seeded on standard 10% milk agar. For the research of psychrotrophic and thermoduric mesophilic microorganisms, the samples were submitted to heat treatment (62.8 ± 0.5 °C) and cooled to 10 ºC. The determination of the Total Bacteria Count, converted to Standard Plate Count (SPC), and Somatic Cell Count (SCC) were performed by the flow cytometry method in automated equipment. The median of farmers of the tanks evaluated was 5, varying from 2 to 19. Of the 23 tanks evaluated, 65.2% had SPC above 300,000 CFU/mL, the current limit defined in legislation. The median of SPC results was 415,000 CFU/mL for tank samples from the Ji-Paraná micro-region and 555,000 CFU/mL for tanks in the Cacoal micro-region, with no statistical difference in SPC between regions. The median count of mesophilic, psychrotrophic, proteolytic psychrotrophic, coliform, mesophilic and psychrotrophic thermoduric were 2.8x105 CFU/mL, 6.8x105 CFU/mL, 4.6x105 CFU/mL, 2.0x104 CFU/mL, 1,5x103 CFU/mL and 1.3 x103 CFU/mL, respectively. The result of this work revealed high deteriorating bacteria counts in raw milk stored in collective tanks, indicating the occurrence of deleterious effects to the milk and dairy products, and economic and technological losses for the regional industry. Defining specific strategies, prioritizing in the adoption of good practices in milking and adaptations in logistic, structure and processes for the milk cooling is fundamental for the microbiological contamination reduction in raw material and adequacy to legislation.
650 $aCold storage
650 $aMicroorganisms
650 $aMilk quality
650 $aArmazenamento
650 $aLeite
650 $aQualidade
650 $aResfriamento
650 $aTanque
653 $aCooling tank
653 $aMicrobiota
700 1 $aOLIVEIRA, A. M. de
700 1 $aMACEDO, S. C. C.
700 1 $aFARIA, G. V.
700 1 $aCONSTANTINO, N. A. S.
700 1 $aSILVA, F. de A. C.
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Registro original: |
Embrapa Rondônia (CPAF-RO) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
29/10/2021 |
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Data da última atualização: |
29/10/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
DINIZ, J. H. W.; PERES, R. F. G.; TEIXEIRA, A. C. B.; RIVEROS, J. A. N.; NORONHA, I. M.; MARTINS, C. F. G.; OLIVEIRA, C. S.; POHLER, K. G.; PUGLIESI, G.; OLIVEIRA, L. Z. |
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Afiliação: |
JULIANA HORTA WILKE DINIZ, Universidade Federal de Minas Gerais; ROGERIO FONSECA GUIMARÃES PERES; ANA CAROLINA BAHIA TEIXEIRA, Universidade Federal de Minas Gerais; JOSE ANDRES NIVIA RIVEROS, Universidade Federal de Minas Gerais; ISABELLA MARCONATO NORONHA; CÍCERO FLEURY GUEDES MARTINS; CLARA SLADE OLIVEIRA, CNPGL; KY GARRETT POHLER, Texas A&M University; GUILHERME PUGLIESI, Universidade de São Paulo; LETICIA ZOCCOLARO OLIVEIRA, Universidade Federal de Minas Gerais. |
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Título: |
Administration of PGF2 alpha at the moment of timed-AI using sex-sortedor conventional semen in suckled nelore cows with different intensityof estrus behavior. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Theriogenology, v. 74, 169e175, 2021. |
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DOI: |
https://doi.org/10.1016/j.theriogenology.2021.08.023 |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this work was to evaluate pregnancy rates (PR) and ovulatory characteristics of Nelore cows receiving PGF2a at the time of AI (artificial insemination) in a progesterone(P4)/estradiol-based timed-AI protocol. We also compared the effects of PGF2a treatment at AI in cows inseminated with conventional or sex-sorted semen, with the absence or expression of estrus. In experiment 1, a total of 701 suckled, multiparous Nelore cows from two commercial beef farms were submitted to the same protocol. All cows received a 12.5 mg (IM) injection of dinoprost tromethamine (Dinoprost; Lutalyse®; PGF treatment) at days 7 and 9 of a timed-AI protocol. Following P4 device removal (day 11; D11), AI was performed 48 h later with conventional or sex-sorted semen from two different sires. At AI, cows received an additional dose of 12.5 mg (IM) of Dinoprost (PGF treatment) or 2.5 mL (IM) of sterile saline (Control). Estrus behavior was determined at D11 by activation of an estrus detection device (Estrotect®). The overall PR was 32.8% (n ¼ 348) at Farm 1 and 42.3% (n ¼ 353) at Farm 2 (P ¼ 0.01). Despite PR differences between farms, the same factors affected PR at Farms 1 and 2. Body condition score (P ¼ 0.02), estrus behavior (P ¼ 0.01), and type of semen (P < 0.001) were factors affecting PR. Conventional semen had a 2.73x greater chance of successful pregnancy than sex-sorted semen. Cows displaying estrus had a 2.5x greater chance of successful pregnancy than cows that did not display estrus. No treatment effect (P ¼ 0.67) was detected in cows receiving conventional or sex-sorted semen. However, there was a tendency (P ¼ 0.08) for an interaction between treatment (PGF or control) and estrus behavior (estrus or no estrus). PGF2a at the time of AI tended to increase PR of cows that did not display estrus (P < 0.10). In experiment 2, 29 suckled, multiparous Nelore cows were compared using B-mode and Doppler ultrasongraphy to assess the ovulatory characteristics of cows receiving the 12.5 mg (IM) injection of Dinoprost (PGF treatment) or saline solution (control) at D11. No significant effects of PGF2a treatment at D11 were observed in follicular characteristics and/or ovulation performance. It was concluded that fertility of sex-sorted semen was lower than conventional semen, regardless of the PGF2a treatment. The 12.5 mg treatment of Dinoprost at AI did not accelerate the occurrence of ovulation; however, it was interesting to note that PGF2a treatment at timed-AI appeared to increase the fertility of cows that did not display estrus, independent of semen type. MenosThe aim of this work was to evaluate pregnancy rates (PR) and ovulatory characteristics of Nelore cows receiving PGF2a at the time of AI (artificial insemination) in a progesterone(P4)/estradiol-based timed-AI protocol. We also compared the effects of PGF2a treatment at AI in cows inseminated with conventional or sex-sorted semen, with the absence or expression of estrus. In experiment 1, a total of 701 suckled, multiparous Nelore cows from two commercial beef farms were submitted to the same protocol. All cows received a 12.5 mg (IM) injection of dinoprost tromethamine (Dinoprost; Lutalyse®; PGF treatment) at days 7 and 9 of a timed-AI protocol. Following P4 device removal (day 11; D11), AI was performed 48 h later with conventional or sex-sorted semen from two different sires. At AI, cows received an additional dose of 12.5 mg (IM) of Dinoprost (PGF treatment) or 2.5 mL (IM) of sterile saline (Control). Estrus behavior was determined at D11 by activation of an estrus detection device (Estrotect®). The overall PR was 32.8% (n ¼ 348) at Farm 1 and 42.3% (n ¼ 353) at Farm 2 (P ¼ 0.01). Despite PR differences between farms, the same factors affected PR at Farms 1 and 2. Body condition score (P ¼ 0.02), estrus behavior (P ¼ 0.01), and type of semen (P < 0.001) were factors affecting PR. Conventional semen had a 2.73x greater chance of successful pregnancy than sex-sorted semen. Cows displaying estrus had a 2.5x greater chance of successful pregnancy than cows that did not displ... Mostrar Tudo |
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Palavras-Chave: |
Dinoprost; Estrus behavior; ProstaglandinF2alpha; Sexed semen; Timed-AI. |
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Thesagro: |
Bovino; Inseminação Artificial; Prenhez; Reprodução Animal; Vaca. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03711naa a2200361 a 4500
001 2135711
005 2021-10-29
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.theriogenology.2021.08.023$2DOI
100 1 $aDINIZ, J. H. W.
245 $aAdministration of PGF2 alpha at the moment of timed-AI using sex-sortedor conventional semen in suckled nelore cows with different intensityof estrus behavior.$h[electronic resource]
260 $c2021
520 $aThe aim of this work was to evaluate pregnancy rates (PR) and ovulatory characteristics of Nelore cows receiving PGF2a at the time of AI (artificial insemination) in a progesterone(P4)/estradiol-based timed-AI protocol. We also compared the effects of PGF2a treatment at AI in cows inseminated with conventional or sex-sorted semen, with the absence or expression of estrus. In experiment 1, a total of 701 suckled, multiparous Nelore cows from two commercial beef farms were submitted to the same protocol. All cows received a 12.5 mg (IM) injection of dinoprost tromethamine (Dinoprost; Lutalyse®; PGF treatment) at days 7 and 9 of a timed-AI protocol. Following P4 device removal (day 11; D11), AI was performed 48 h later with conventional or sex-sorted semen from two different sires. At AI, cows received an additional dose of 12.5 mg (IM) of Dinoprost (PGF treatment) or 2.5 mL (IM) of sterile saline (Control). Estrus behavior was determined at D11 by activation of an estrus detection device (Estrotect®). The overall PR was 32.8% (n ¼ 348) at Farm 1 and 42.3% (n ¼ 353) at Farm 2 (P ¼ 0.01). Despite PR differences between farms, the same factors affected PR at Farms 1 and 2. Body condition score (P ¼ 0.02), estrus behavior (P ¼ 0.01), and type of semen (P < 0.001) were factors affecting PR. Conventional semen had a 2.73x greater chance of successful pregnancy than sex-sorted semen. Cows displaying estrus had a 2.5x greater chance of successful pregnancy than cows that did not display estrus. No treatment effect (P ¼ 0.67) was detected in cows receiving conventional or sex-sorted semen. However, there was a tendency (P ¼ 0.08) for an interaction between treatment (PGF or control) and estrus behavior (estrus or no estrus). PGF2a at the time of AI tended to increase PR of cows that did not display estrus (P < 0.10). In experiment 2, 29 suckled, multiparous Nelore cows were compared using B-mode and Doppler ultrasongraphy to assess the ovulatory characteristics of cows receiving the 12.5 mg (IM) injection of Dinoprost (PGF treatment) or saline solution (control) at D11. No significant effects of PGF2a treatment at D11 were observed in follicular characteristics and/or ovulation performance. It was concluded that fertility of sex-sorted semen was lower than conventional semen, regardless of the PGF2a treatment. The 12.5 mg treatment of Dinoprost at AI did not accelerate the occurrence of ovulation; however, it was interesting to note that PGF2a treatment at timed-AI appeared to increase the fertility of cows that did not display estrus, independent of semen type.
650 $aBovino
650 $aInseminação Artificial
650 $aPrenhez
650 $aReprodução Animal
650 $aVaca
653 $aDinoprost
653 $aEstrus behavior
653 $aProstaglandinF2alpha
653 $aSexed semen
653 $aTimed-AI
700 1 $aPERES, R. F. G.
700 1 $aTEIXEIRA, A. C. B.
700 1 $aRIVEROS, J. A. N.
700 1 $aNORONHA, I. M.
700 1 $aMARTINS, C. F. G.
700 1 $aOLIVEIRA, C. S.
700 1 $aPOHLER, K. G.
700 1 $aPUGLIESI, G.
700 1 $aOLIVEIRA, L. Z.
773 $tTheriogenology$gv. 74, 169e175, 2021.
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