| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Uva e Vinho. |
|
Data corrente: |
25/10/2017 |
|
Data da última atualização: |
06/05/2019 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
MOURA, C. J. M. de; FAJARDO, T. V. M.; EIRAS, M.; SILVA, F. N. da; NICKEL, O. |
|
Afiliação: |
Cátia Jacira Martins de Moura, 1Biological Institute ? Dept. of Plant Pathology ? Lab. of Plant Virology, Av. Conselheiro Rodrigues Alves, 1252 ? 04014-900 ? São Paulo, SP ? Brazil.; THOR VINICIUS MARTINS FAJARDO, CNPUV; Marcelo Eiras, 1Biological Institute ? Dept. of Plant Pathology ? Lab. of Plant Virology, Av. Conselheiro Rodrigues Alves, 1252 ? 04014-900 ? São Paulo, SP ? Brazil.; Fábio Nascimento da Silva, 3Santa Catarina State University/Centre of Agroveterinary Sciences, Av. Luiz de Camões, 2090 ? 88520-000 ? Lages, SC ? Brazil.; OSMAR NICKEL, CNPUV. |
|
Título: |
Molecular characterization of GSyV-1 and GLRaV-3 and prevalence of grapevine viruses in a grape-growing area. |
|
Ano de publicação: |
2018 |
|
Fonte/Imprenta: |
Scientia Agricola, Brasília,DF, v. 75, n. 1, p.43-51, jan./feb. 2018. |
|
DOI: |
10.1590/1678-992X-2016-0328 |
|
Idioma: |
Inglês |
|
Conteúdo: |
The aims of this study were to determine the prevalence of viruses in 119 samples from 32 grapevine cultivars, collected from nine vineyards in a specific grape-growing area in southeastern Brazil, perform a partial molecular characterization of 14 isolates of Grapevine Syrah virus 1 (GSyV-1) and Grapevine leafroll-associated virus 3 (GLRaV-3) and assess the coat protein genetic variability of these viruses. The detection of viruses was implemented by realtime RT-PCR (reverse transcription polymerase chain reaction) aiming to detect seven viruses and one viroid. With the exception of the Grapevine Cabernet Sauvignon reovirus (GCSV), the viruses and viroid that were evaluated were widespread in the sampled areas, often in high prevalence and multiple infections, ranging from 15 % up to 76 %. Eight isolates of GSyV-1 and six of GLRaV-3, partially characterized by complete coat protein gene nucleotide sequencing and a variability study showed nucleotide identities ranging from 91 % to 99 % (GSyV-1) and from 98 % to 100 % (GLRaV-3) among themselves, respectively. Comparisons between conventional and real-time RT-PCR detections were implemented for GSyV-1 and GLRaV-3 infections. Analysis of genetic variability indicated molecular differences between GSyV-1 and GLRaV-3 isolates and negative selection acting on the coat protein gene of both viruses. This is the first report of GSyV-1 in commercial vineyards in Brazil. The survey revealed widespread infections of seven important pathogens in one prominent Brazilian grape-producing region implying contaminated grapevine cuttings in the spread of disease. Keywords: Vitis, diagnosis, variability, incidence, leafroll MenosThe aims of this study were to determine the prevalence of viruses in 119 samples from 32 grapevine cultivars, collected from nine vineyards in a specific grape-growing area in southeastern Brazil, perform a partial molecular characterization of 14 isolates of Grapevine Syrah virus 1 (GSyV-1) and Grapevine leafroll-associated virus 3 (GLRaV-3) and assess the coat protein genetic variability of these viruses. The detection of viruses was implemented by realtime RT-PCR (reverse transcription polymerase chain reaction) aiming to detect seven viruses and one viroid. With the exception of the Grapevine Cabernet Sauvignon reovirus (GCSV), the viruses and viroid that were evaluated were widespread in the sampled areas, often in high prevalence and multiple infections, ranging from 15 % up to 76 %. Eight isolates of GSyV-1 and six of GLRaV-3, partially characterized by complete coat protein gene nucleotide sequencing and a variability study showed nucleotide identities ranging from 91 % to 99 % (GSyV-1) and from 98 % to 100 % (GLRaV-3) among themselves, respectively. Comparisons between conventional and real-time RT-PCR detections were implemented for GSyV-1 and GLRaV-3 infections. Analysis of genetic variability indicated molecular differences between GSyV-1 and GLRaV-3 isolates and negative selection acting on the coat protein gene of both viruses. This is the first report of GSyV-1 in commercial vineyards in Brazil. The survey revealed widespread infections of seven important pat... Mostrar Tudo |
|
Palavras-Chave: |
Caracterização molecular; Cultivo de uva; Diagnosis; Leafroll; Variabilitty; Videira; Vírus em videira. |
|
Thesagro: |
DOença de planta; Uva. |
|
Thesaurus Nal: |
incidence; Vitis. |
|
Categoria do assunto: |
A Sistemas de Cultivo |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/165554/1/SA-Molecular-characterization-GSyV1-and-GLRaV3-2018.pdf
|
|
Marc: |
LEADER 02596naa a2200313 a 4500
001 2078147
005 2019-05-06
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1590/1678-992X-2016-0328$2DOI
100 1 $aMOURA, C. J. M. de
245 $aMolecular characterization of GSyV-1 and GLRaV-3 and prevalence of grapevine viruses in a grape-growing area.$h[electronic resource]
260 $c2018
520 $aThe aims of this study were to determine the prevalence of viruses in 119 samples from 32 grapevine cultivars, collected from nine vineyards in a specific grape-growing area in southeastern Brazil, perform a partial molecular characterization of 14 isolates of Grapevine Syrah virus 1 (GSyV-1) and Grapevine leafroll-associated virus 3 (GLRaV-3) and assess the coat protein genetic variability of these viruses. The detection of viruses was implemented by realtime RT-PCR (reverse transcription polymerase chain reaction) aiming to detect seven viruses and one viroid. With the exception of the Grapevine Cabernet Sauvignon reovirus (GCSV), the viruses and viroid that were evaluated were widespread in the sampled areas, often in high prevalence and multiple infections, ranging from 15 % up to 76 %. Eight isolates of GSyV-1 and six of GLRaV-3, partially characterized by complete coat protein gene nucleotide sequencing and a variability study showed nucleotide identities ranging from 91 % to 99 % (GSyV-1) and from 98 % to 100 % (GLRaV-3) among themselves, respectively. Comparisons between conventional and real-time RT-PCR detections were implemented for GSyV-1 and GLRaV-3 infections. Analysis of genetic variability indicated molecular differences between GSyV-1 and GLRaV-3 isolates and negative selection acting on the coat protein gene of both viruses. This is the first report of GSyV-1 in commercial vineyards in Brazil. The survey revealed widespread infections of seven important pathogens in one prominent Brazilian grape-producing region implying contaminated grapevine cuttings in the spread of disease. Keywords: Vitis, diagnosis, variability, incidence, leafroll
650 $aincidence
650 $aVitis
650 $aDOença de planta
650 $aUva
653 $aCaracterização molecular
653 $aCultivo de uva
653 $aDiagnosis
653 $aLeafroll
653 $aVariabilitty
653 $aVideira
653 $aVírus em videira
700 1 $aFAJARDO, T. V. M.
700 1 $aEIRAS, M.
700 1 $aSILVA, F. N. da
700 1 $aNICKEL, O.
773 $tScientia Agricola, Brasília,DF$gv. 75, n. 1, p.43-51, jan./feb. 2018.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Uva e Vinho (CNPUV) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Meio-Norte. |
|
Data corrente: |
23/12/2015 |
|
Data da última atualização: |
24/02/2016 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 2 |
|
Autoria: |
AMARAL, M. R. X.; ALBRECHT, M.; MCKINLEY, A. S.; CARVALHO, A. M. F. de; SOUSA JUNIOR, S. C. de; DINIZ, F. M. |
|
Afiliação: |
MARIA ROSIMERE XAVIER AMARAL, CPAMN; MARC ALBRECHT, University of Nebraska at Kearney, Kearney, NE 68849, USA.; ALAN SHANE MCKINLEY, National Park Service, 18001 Old Cutler Bay Rd Suite 419, Palmetto Bay, FL 33190, USA.; ADRIANA MÁRCIA FERREIRA DE CARVALHO, CPAMN; SEVERINO CAVALCANTE DE SOUSA JUNIOR, Universidade Federal do Piauí, Bom Jesus, PI, Brazil.; FABIO MENDONCA DINIZ, CPAMN. |
|
Título: |
Mitochondrial DNA variation reveals a sharp genetic break within the distribution of the blue land crab cardisoma Guanhumi in the Western Central Atlantic. |
|
Ano de publicação: |
2015 |
|
Fonte/Imprenta: |
Molecules, v. 20, p. 15158-15174, 2015. |
|
ISSN: |
1420-3049 |
|
DOI: |
10.3390/molecules200815158 |
|
Idioma: |
Inglês |
|
Conteúdo: |
The blue land crab Cardisoma guanhumi is widely distributed throughout tropical and subtropical estuarine regions in the Western Central Atlantic (WCA)... |
|
Palavras-Chave: |
Diversidade genética. |
|
Thesagro: |
Caranguejo. |
|
Categoria do assunto: |
G Melhoramento Genético |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/136123/1/ArtigoFabioDinizMoleculesDez2015.pdf
|
|
Marc: |
LEADER 00904naa a2200229 a 4500
001 2032378
005 2016-02-24
008 2015 bl uuuu u00u1 u #d
022 $a1420-3049
024 7 $a10.3390/molecules200815158$2DOI
100 1 $aAMARAL, M. R. X.
245 $aMitochondrial DNA variation reveals a sharp genetic break within the distribution of the blue land crab cardisoma Guanhumi in the Western Central Atlantic.$h[electronic resource]
260 $c2015
520 $aThe blue land crab Cardisoma guanhumi is widely distributed throughout tropical and subtropical estuarine regions in the Western Central Atlantic (WCA)...
650 $aCaranguejo
653 $aDiversidade genética
700 1 $aALBRECHT, M.
700 1 $aMCKINLEY, A. S.
700 1 $aCARVALHO, A. M. F. de
700 1 $aSOUSA JUNIOR, S. C. de
700 1 $aDINIZ, F. M.
773 $tMolecules$gv. 20, p. 15158-15174, 2015.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Meio-Norte (CPAMN) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
