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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
30/07/2014 |
Data da última atualização: |
25/05/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MENEZES, S. P.; SILVA, D. M. de A.; LIMA, E. M.; SOUSA, A. O. de; ANDRADE, B. S.; LEMOS, L. S. L.; GRAMACHO, A. P.; GESTEIRA, A. da S.; PIROVANI, C. P.; MICHELI, F. |
Afiliação: |
SARA PEREIRA MENEZES, UESC; DSON MARIO DE ANDRADE SILVA, UESC; ELINE MATOS LIMA, UESC; AURIZÂNGELA OLIVEIRA DE SOUSA, UESC; BRUNO SILVA ANDRADE; LIVIA SANTOS LIMA LEMOS; ARINA PERES GRAMACHO; ABELMON DA SILVA GESTEIRA, CNPMF; CARLOS PRIMINHO PIROVANI; FABIENNE MICHELI, UESC. |
Título: |
The pathogenesis-related protein PR-4b from Theobroma cacao presents RNase activity, Ca2+ and Mg2+ dependent-DNase activity and antifungal action on Moniliophthora pernicios. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
BMC Plant Biology, 2014. |
ISSN: |
1471-2229 |
Idioma: |
Inglês |
Conteúdo: |
Background: The production and accumulation of pathogenesis-related proteins (PR proteins) in plants in response to biotic or abiotic stresses is well known and is considered as a crucial mechanism for plant defense. A pathogenesis-related protein 4 cDNA was identified from a cacao-Moniliophthora perniciosa interaction cDNA library and named TcPR-4b. Results: TcPR-4b presents a Barwin domain with six conserved cysteine residues, but lacks the chitin-binding site. Molecular modeling of TcPR-4b confirmed the importance of the cysteine residues to maintain the protein structure, and of several conserved amino acids for the catalytic activity. In the cacao genome, TcPR-4b belonged to a small multigene family organized mainly on chromosome 5. TcPR-4b RT-qPCR analysis in resistant and susceptible cacao plants infected by M. perniciosa showed an increase of expression at 48 hours after infection (hai) in both cacao genotypes. After the initial stage (24-72 hai), the TcPR-4b expression was observed at all times in the resistant genotypes, while in the susceptible one the expression was concentrated at the final stages of infection (45-90 days after infection).The recombinant TcPR-4b protein showed RNase, and bivalent ions dependent-DNase activity, but no chitinase activity. Moreover, TcPR-4b presented antifungal action against M. perniciosa, and the reduction of M. perniciosa survival was related to ROS production in fungal hyphae. Conclusion: To our knowledge, this is the first report of a PR-4 showing simultaneously RNase, DNase and antifungal properties, but no chitinase activity. Moreover, we showed that the antifungal activity of TcPR-4b is directly related to RNase function. In cacao, TcPR-4b nuclease activities may be related to the establishment and maintenance of resistance, and to the PCD mechanism, in resistant and susceptible cacao genotypes, respectively. MenosBackground: The production and accumulation of pathogenesis-related proteins (PR proteins) in plants in response to biotic or abiotic stresses is well known and is considered as a crucial mechanism for plant defense. A pathogenesis-related protein 4 cDNA was identified from a cacao-Moniliophthora perniciosa interaction cDNA library and named TcPR-4b. Results: TcPR-4b presents a Barwin domain with six conserved cysteine residues, but lacks the chitin-binding site. Molecular modeling of TcPR-4b confirmed the importance of the cysteine residues to maintain the protein structure, and of several conserved amino acids for the catalytic activity. In the cacao genome, TcPR-4b belonged to a small multigene family organized mainly on chromosome 5. TcPR-4b RT-qPCR analysis in resistant and susceptible cacao plants infected by M. perniciosa showed an increase of expression at 48 hours after infection (hai) in both cacao genotypes. After the initial stage (24-72 hai), the TcPR-4b expression was observed at all times in the resistant genotypes, while in the susceptible one the expression was concentrated at the final stages of infection (45-90 days after infection).The recombinant TcPR-4b protein showed RNase, and bivalent ions dependent-DNase activity, but no chitinase activity. Moreover, TcPR-4b presented antifungal action against M. perniciosa, and the reduction of M. perniciosa survival was related to ROS production in fungal hyphae. Conclusion: To our knowledge, this is the first rep... Mostrar Tudo |
Thesagro: |
Cacau; Theobroma Cacao. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02703naa a2200265 a 4500 001 1991551 005 2023-05-25 008 2014 bl uuuu u00u1 u #d 022 $a1471-2229 100 1 $aMENEZES, S. P. 245 $aThe pathogenesis-related protein PR-4b from Theobroma cacao presents RNase activity, Ca2+ and Mg2+ dependent-DNase activity and antifungal action on Moniliophthora pernicios.$h[electronic resource] 260 $c2014 520 $aBackground: The production and accumulation of pathogenesis-related proteins (PR proteins) in plants in response to biotic or abiotic stresses is well known and is considered as a crucial mechanism for plant defense. A pathogenesis-related protein 4 cDNA was identified from a cacao-Moniliophthora perniciosa interaction cDNA library and named TcPR-4b. Results: TcPR-4b presents a Barwin domain with six conserved cysteine residues, but lacks the chitin-binding site. Molecular modeling of TcPR-4b confirmed the importance of the cysteine residues to maintain the protein structure, and of several conserved amino acids for the catalytic activity. In the cacao genome, TcPR-4b belonged to a small multigene family organized mainly on chromosome 5. TcPR-4b RT-qPCR analysis in resistant and susceptible cacao plants infected by M. perniciosa showed an increase of expression at 48 hours after infection (hai) in both cacao genotypes. After the initial stage (24-72 hai), the TcPR-4b expression was observed at all times in the resistant genotypes, while in the susceptible one the expression was concentrated at the final stages of infection (45-90 days after infection).The recombinant TcPR-4b protein showed RNase, and bivalent ions dependent-DNase activity, but no chitinase activity. Moreover, TcPR-4b presented antifungal action against M. perniciosa, and the reduction of M. perniciosa survival was related to ROS production in fungal hyphae. Conclusion: To our knowledge, this is the first report of a PR-4 showing simultaneously RNase, DNase and antifungal properties, but no chitinase activity. Moreover, we showed that the antifungal activity of TcPR-4b is directly related to RNase function. In cacao, TcPR-4b nuclease activities may be related to the establishment and maintenance of resistance, and to the PCD mechanism, in resistant and susceptible cacao genotypes, respectively. 650 $aCacau 650 $aTheobroma Cacao 700 1 $aSILVA, D. M. de A. 700 1 $aLIMA, E. M. 700 1 $aSOUSA, A. O. de 700 1 $aANDRADE, B. S. 700 1 $aLEMOS, L. S. L. 700 1 $aGRAMACHO, A. P. 700 1 $aGESTEIRA, A. da S. 700 1 $aPIROVANI, C. P. 700 1 $aMICHELI, F. 773 $tBMC Plant Biology, 2014.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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Biblioteca(s): |
Embrapa Hortaliças; Embrapa Meio-Norte. |
Data corrente: |
13/12/2012 |
Data da última atualização: |
13/12/2012 |
Tipo da produção científica: |
Circular Técnica |
Autoria: |
MAROUELLI, W. A.; BRAGA, M. B.; ANDRADE JUNIOR, A. S. de. |
Afiliação: |
WALDIR APARECIDO MAROUELLI, CNPH; MARCOS BRANDAO BRAGA, CNPH; ADERSON SOARES DE ANDRADE JUNIOR, CPAMN. |
Título: |
Irrigação na cultura da melancia. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
Brasília, DF: Embrapa, 2012. |
Série: |
(Embrapa Hortaliças. Circular técnica, 108). |
ISSN: |
1415-3033 |
Idioma: |
Português |
Palavras-Chave: |
Irrigação subsuperficial. |
Thesagro: |
Irrigação localizada; Irrigação por aspersão; Irrigação por sulco; Melancia. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/72287/1/ct-1081.pdf
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Marc: |
LEADER 00621nam a2200205 a 4500 001 1942564 005 2012-12-13 008 2012 bl uuuu u0uu1 u #d 022 $a1415-3033 100 1 $aMAROUELLI, W. A. 245 $aIrrigação na cultura da melancia.$h[electronic resource] 260 $aBrasília, DF: Embrapa$c2012 490 $a(Embrapa Hortaliças. Circular técnica, 108). 650 $aIrrigação localizada 650 $aIrrigação por aspersão 650 $aIrrigação por sulco 650 $aMelancia 653 $aIrrigação subsuperficial 700 1 $aBRAGA, M. B. 700 1 $aANDRADE JUNIOR, A. S. de
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