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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
27/12/2021 |
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Data da última atualização: |
27/12/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PAIXÃO, F. R. S. da; HUARTE BONNET, C.; RIBEIRO-SILVA, C. de S.; MASCARIN, G. M.; FERNANDES, E. K. K.; PEDRINI, N. |
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Afiliação: |
FLÁVIA REGINA SANTOS DA PAIXÃO, Universidad Nacional de La Plata; CARLA HUARTE BONNET, Universidad Nacional de La Plata; CÁRITA SOUZA RIBEIRO-SILVA, UFG; GABRIEL MOURA MASCARIN, CNPMA; ÉVERTON KORT KAMP FERNANDES, UFG. |
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Título: |
Tolerance to abiotic factors of microsclerotia and mycelial pellets from Metarhizium robertsii, and molecular and ultrastructural changes during microsclerotial differentiation. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Frontiers in Fungal Biology, v. 2, Article 654737, 2021. |
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DOI: |
https://doi.org/10.3389/ffunb.2021.654737 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Metarhizium species fungi are able to produce resistant structures termed microsclerotia, formed by compact and melanized threads of hyphae. These propagules are tolerant to desiccation and produce infective conidia; thus, they are promising candidates to use in biological control programs. In this study, we investigated the tolerance to both ultraviolet B (UV-B) radiation and heat of microsclerotia of Metarhizium robertsii strain ARSEF 2575. We also adapted the liquid medium and culture conditions to obtain mycelial pellets from the same isolate in order to compare these characteristics between both types of propagules. We followed the peroxisome biogenesis and studied the oxidative stress during differentiation from conidia to microsclerotia by transmission electron microscopy after staining with a peroxidase activity marker and by the expression pattern of genes potentially involved in these processes. We found that despite their twice smaller size, microsclerotia exhibited higher dry biomass, yield, and conidial productivity than mycelial pellets, both with and without UV-B and heat stresses. From the 16 genes measured, we found an induction after 96-h differentiation in the oxidative stress marker genes MrcatA, MrcatP, and Mrgpx; the peroxisome biogenesis factors Mrpex5 and Mrpex14/17; and the photoprotection genes Mrlac1 and Mrlac2; and Mrlac3. We concluded that an oxidative stress scenario is induced during microsclerotia differentiation in M. robertsii and confirmed that because of its tolerance to desiccation, heat, and UV-B, this fungal structure could be an excellent candidate for use in biological control of pests under tropical and subtropical climates where heat and UV radiation are detrimental to entomopathogenic fungi survival and persistence. MenosAbstract: Metarhizium species fungi are able to produce resistant structures termed microsclerotia, formed by compact and melanized threads of hyphae. These propagules are tolerant to desiccation and produce infective conidia; thus, they are promising candidates to use in biological control programs. In this study, we investigated the tolerance to both ultraviolet B (UV-B) radiation and heat of microsclerotia of Metarhizium robertsii strain ARSEF 2575. We also adapted the liquid medium and culture conditions to obtain mycelial pellets from the same isolate in order to compare these characteristics between both types of propagules. We followed the peroxisome biogenesis and studied the oxidative stress during differentiation from conidia to microsclerotia by transmission electron microscopy after staining with a peroxidase activity marker and by the expression pattern of genes potentially involved in these processes. We found that despite their twice smaller size, microsclerotia exhibited higher dry biomass, yield, and conidial productivity than mycelial pellets, both with and without UV-B and heat stresses. From the 16 genes measured, we found an induction after 96-h differentiation in the oxidative stress marker genes MrcatA, MrcatP, and Mrgpx; the peroxisome biogenesis factors Mrpex5 and Mrpex14/17; and the photoprotection genes Mrlac1 and Mrlac2; and Mrlac3. We concluded that an oxidative stress scenario is induced during microsclerotia differentiation in M. robertsii and ... Mostrar Tudo |
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Palavras-Chave: |
Thermotolerance; Tolerância; UV-B radiation. |
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Thesagro: |
Controle Biológico; Fungo Entomógeno; Fungo Para Controle Biológico; Radiação Solar; Raio Ultravioleta. |
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Thesaurus Nal: |
Biological control; Entomopathogenic fungi; Gene expression; Heat tolerance; Metarhizium robertsii; Oxidative stress; Ultraviolet radiation. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/229681/1/Mascarin-Tolerance-abiotic-2021.pdf
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Marc: |
LEADER 03040naa a2200373 a 4500
001 2138387
005 2021-12-27
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3389/ffunb.2021.654737$2DOI
100 1 $aPAIXÃO, F. R. S. da
245 $aTolerance to abiotic factors of microsclerotia and mycelial pellets from Metarhizium robertsii, and molecular and ultrastructural changes during microsclerotial differentiation.$h[electronic resource]
260 $c2021
520 $aAbstract: Metarhizium species fungi are able to produce resistant structures termed microsclerotia, formed by compact and melanized threads of hyphae. These propagules are tolerant to desiccation and produce infective conidia; thus, they are promising candidates to use in biological control programs. In this study, we investigated the tolerance to both ultraviolet B (UV-B) radiation and heat of microsclerotia of Metarhizium robertsii strain ARSEF 2575. We also adapted the liquid medium and culture conditions to obtain mycelial pellets from the same isolate in order to compare these characteristics between both types of propagules. We followed the peroxisome biogenesis and studied the oxidative stress during differentiation from conidia to microsclerotia by transmission electron microscopy after staining with a peroxidase activity marker and by the expression pattern of genes potentially involved in these processes. We found that despite their twice smaller size, microsclerotia exhibited higher dry biomass, yield, and conidial productivity than mycelial pellets, both with and without UV-B and heat stresses. From the 16 genes measured, we found an induction after 96-h differentiation in the oxidative stress marker genes MrcatA, MrcatP, and Mrgpx; the peroxisome biogenesis factors Mrpex5 and Mrpex14/17; and the photoprotection genes Mrlac1 and Mrlac2; and Mrlac3. We concluded that an oxidative stress scenario is induced during microsclerotia differentiation in M. robertsii and confirmed that because of its tolerance to desiccation, heat, and UV-B, this fungal structure could be an excellent candidate for use in biological control of pests under tropical and subtropical climates where heat and UV radiation are detrimental to entomopathogenic fungi survival and persistence.
650 $aBiological control
650 $aEntomopathogenic fungi
650 $aGene expression
650 $aHeat tolerance
650 $aMetarhizium robertsii
650 $aOxidative stress
650 $aUltraviolet radiation
650 $aControle Biológico
650 $aFungo Entomógeno
650 $aFungo Para Controle Biológico
650 $aRadiação Solar
650 $aRaio Ultravioleta
653 $aThermotolerance
653 $aTolerância
653 $aUV-B radiation
700 1 $aHUARTE BONNET, C.
700 1 $aRIBEIRO-SILVA, C. de S.
700 1 $aMASCARIN, G. M.
700 1 $aFERNANDES, E. K. K.
700 1 $aPEDRINI, N.
773 $tFrontiers in Fungal Biology$gv. 2, Article 654737, 2021.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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Classificação |
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Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Acre. |
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Data corrente: |
15/04/1997 |
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Data da última atualização: |
23/05/2023 |
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Autoria: |
VALENTIM, J. F.; COSTA, A. L. da. |
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Afiliação: |
JUDSON FERREIRA VALENTIM, CPAF-AC; ARLINDO LUIZ DA COSTA, CPAF-AC. |
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Título: |
Adaptação de leguminosas forrageiras consorciadas com gramíneas no Acre. |
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Ano de publicação: |
1981 |
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Fonte/Imprenta: |
Rio Branco, AC: Embrapa-UEPAE Rio Branco, 1981. |
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Páginas: |
3 p. |
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Série: |
(Embrapa-UEPAE Rio Branco. Pesquisa em andamento, 4). |
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Idioma: |
Português |
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Conteúdo: |
A Unidade de Execução de Pesquisa de Âmbito Estadual - UEPAB/RIO BRANCO, através do Projeto de Melhoramento de Pastagens da Amazônia Legal-PROPASTO/AMAZONIA, vem desenvolvendo, no município de Senador Guiomard, estudos objetivando avaliar a adaptação de 15 leguminosas forrageiras, sendo 11 selecionadas pelo Centro Internacional de Agricultura Tropical-CIAT, para as condições do trópico úmido e 4 comerciais, consideradas como promissoras para o Estado. |
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Palavras-Chave: |
Aclimatación; Acre; Amazonia Occidental; Amazônia Ocidental; Brasil; Consorciação; Cultivo intercalar; Ensayos de variedades; Factores ambientales; Forage plant; Gramíneas; Legumes plant; Leguminosas forrajeras; Pastos forrajeros; Planta leguminosa; Plantas gramineas; Rendimiento de los cultivos; Senador Guiomard (AC); Western Amazon. |
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Thesagro: |
Aclimatação; Características Agronômicas; Comportamento de Variedade; Condição Ambiental; Gramínea; Gramínea Forrageira; Leguminosa Forrageira; Melhoramento Genético Vegetal; Pastagem Consorciada; Planta Forrageira; Rendimento. |
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Thesaurus NAL: |
Acclimation; Agronomic traits; Brazil; Crop yield; Environmental factors; Forage grasses; Forage legumes; Intercropping; Plant breeding; Variety trials. |
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Categoria do assunto: |
--
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/117353/1/1086.pdf
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Marc: |
LEADER 02248nam a2200625 a 4500
001 1492355
005 2023-05-23
008 1981 bl uuuu u0uu1 u #d
100 1 $aVALENTIM, J. F.
245 $aAdaptação de leguminosas forrageiras consorciadas com gramíneas no Acre.
260 $aRio Branco, AC: Embrapa-UEPAE Rio Branco$c1981
300 $a3 p.
490 $a(Embrapa-UEPAE Rio Branco. Pesquisa em andamento, 4).
520 $aA Unidade de Execução de Pesquisa de Âmbito Estadual - UEPAB/RIO BRANCO, através do Projeto de Melhoramento de Pastagens da Amazônia Legal-PROPASTO/AMAZONIA, vem desenvolvendo, no município de Senador Guiomard, estudos objetivando avaliar a adaptação de 15 leguminosas forrageiras, sendo 11 selecionadas pelo Centro Internacional de Agricultura Tropical-CIAT, para as condições do trópico úmido e 4 comerciais, consideradas como promissoras para o Estado.
650 $aAcclimation
650 $aAgronomic traits
650 $aBrazil
650 $aCrop yield
650 $aEnvironmental factors
650 $aForage grasses
650 $aForage legumes
650 $aIntercropping
650 $aPlant breeding
650 $aVariety trials
650 $aAclimatação
650 $aCaracterísticas Agronômicas
650 $aComportamento de Variedade
650 $aCondição Ambiental
650 $aGramínea
650 $aGramínea Forrageira
650 $aLeguminosa Forrageira
650 $aMelhoramento Genético Vegetal
650 $aPastagem Consorciada
650 $aPlanta Forrageira
650 $aRendimento
653 $aAclimatación
653 $aAcre
653 $aAmazonia Occidental
653 $aAmazônia Ocidental
653 $aBrasil
653 $aConsorciação
653 $aCultivo intercalar
653 $aEnsayos de variedades
653 $aFactores ambientales
653 $aForage plant
653 $aGramíneas
653 $aLegumes plant
653 $aLeguminosas forrajeras
653 $aPastos forrajeros
653 $aPlanta leguminosa
653 $aPlantas gramineas
653 $aRendimiento de los cultivos
653 $aSenador Guiomard (AC)
653 $aWestern Amazon
700 1 $aCOSTA, A. L. da
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Registro original: |
Embrapa Acre (CPAF-AC) |
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