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Registro Completo |
Biblioteca(s): |
Embrapa Algodão. |
Data corrente: |
03/03/2017 |
Data da última atualização: |
08/03/2017 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
SANTOS, R. C. dos; SILVA, C. R. C. da; SOARES, T. da C; CAVALCANTI, J. J. V.; LIMA, L. M. de; MARTINS, E. S.; PONTES, R. G. M. S. de. |
Afiliação: |
ROSEANE CAVALCANTI DOS SANTOS, CNPA; CARLIANE REBECA COELHO da SILVA, UFRPE; TAÍZA da CUNHA SOARES, UFRPE; JOSE JAIME VASCONCELOS CAVALCANTI, CNPA; LIZIANE MARIA DE LIMA, CNPA; ÉRICA SOARES MARTINS, Cenargen; ROSE GOMES MONNERAT SOLON DE PONTES, Cenargen. |
Título: |
Relative expression of CRy10 in GM cotton lines resistant to boll weevil. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
In: ENCONTRO DE GENÉTICA DO NORDESTE, 21., 2016, Recife. Anais... Ribeirão Preto: SBG; Recife: UFPE: UFRPE: UPE, 2016. p. 87. |
Idioma: |
Inglês |
Conteúdo: |
Boll weevil (Anthonomus grandis) is a serious pest of cotton crop in several parts of world. The control is done by synthetic pesticides that increase the management costs. Embrapa coordinates an important research involving development of GM cotton resistant to boll weevil, by genetic transformation. Seven line (T0-8H) containing a Btcry 10 have been identified by feeding bioassays and ELISA, which showed reasonable toxicity against adult boll weevils. In this work, these lines were used in order to estimate the presence and relative expression of cry 10 by PCR and RT-qPCR, respectively, from young buds of 50d-plants. RT-qPCR assays were performed, by using Syber Green Rox Plus Master Mix 2X (LGC). Three endogenous cotton gene (GhACT, GhUBQ14 and GhPP2A) were used as a standard control. At first, 95°C/15 min and 40 cycles of 95°C/ 20 sec, 60°C/ 20 sec and 72°C/ 20 sec. Then, a curve of denaturation (melting curve) was performed after conclusion of amplification, at 95°C/15 sec, 60°C/15 sec, rising 2°/min until reaching 95°C. All reactions were carried out with experimental and biological replications. The threshold cycle (Ct) and PCR efficiency was estimated by Real-time PCR Miner program. The analyses were performed by using qBASEPlus program. Graphics, Cqs and Melt curve were automatically generated, based on the normalization method with a reference gene, ??Cq. Varied levels of expression were found in GM lines, too low in 8H-269 and 8H-357, mid in 8H-282 and 8H-346 and high in 8H- 336 (14x). These data agreed with previous results obtained by ELISA assays. Eleven lines derivate from 8H-336 (T1) were analyzed by PCR assays with genomic DNA, using 2 primer combinations. More than 50% showed amplicons confirming the presence of gene in selected lines. Taking in account that a reasonable level of resistance should overcoming 2 ug of protein/g tissue, we suggested that 8H-366 is the best genotype for control the cotton boll weevil. This material will be further advanced for entomological assays with larvae and adults of boll weevil. MenosBoll weevil (Anthonomus grandis) is a serious pest of cotton crop in several parts of world. The control is done by synthetic pesticides that increase the management costs. Embrapa coordinates an important research involving development of GM cotton resistant to boll weevil, by genetic transformation. Seven line (T0-8H) containing a Btcry 10 have been identified by feeding bioassays and ELISA, which showed reasonable toxicity against adult boll weevils. In this work, these lines were used in order to estimate the presence and relative expression of cry 10 by PCR and RT-qPCR, respectively, from young buds of 50d-plants. RT-qPCR assays were performed, by using Syber Green Rox Plus Master Mix 2X (LGC). Three endogenous cotton gene (GhACT, GhUBQ14 and GhPP2A) were used as a standard control. At first, 95°C/15 min and 40 cycles of 95°C/ 20 sec, 60°C/ 20 sec and 72°C/ 20 sec. Then, a curve of denaturation (melting curve) was performed after conclusion of amplification, at 95°C/15 sec, 60°C/15 sec, rising 2°/min until reaching 95°C. All reactions were carried out with experimental and biological replications. The threshold cycle (Ct) and PCR efficiency was estimated by Real-time PCR Miner program. The analyses were performed by using qBASEPlus program. Graphics, Cqs and Melt curve were automatically generated, based on the normalization method with a reference gene, ??Cq. Varied levels of expression were found in GM lines, too low in 8H-269 and 8H-357, mid in 8H-282 and 8H-346 and ... Mostrar Tudo |
Palavras-Chave: |
Boll weevil. |
Thesagro: |
Algodão; Anthonomus Grandis; Bicudo. |
Thesaurus Nal: |
Cotton. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/157044/1/Relative-expression-of-Cry10.pdf
|
Marc: |
LEADER 02851nam a2200241 a 4500 001 2066096 005 2017-03-08 008 2016 bl uuuu u00u1 u #d 100 1 $aSANTOS, R. C. dos 245 $aRelative expression of CRy10 in GM cotton lines resistant to boll weevil.$h[electronic resource] 260 $aIn: ENCONTRO DE GENÉTICA DO NORDESTE, 21., 2016, Recife. Anais... Ribeirão Preto: SBG; Recife: UFPE: UFRPE: UPE, 2016. p. 87.$c2016 520 $aBoll weevil (Anthonomus grandis) is a serious pest of cotton crop in several parts of world. The control is done by synthetic pesticides that increase the management costs. Embrapa coordinates an important research involving development of GM cotton resistant to boll weevil, by genetic transformation. Seven line (T0-8H) containing a Btcry 10 have been identified by feeding bioassays and ELISA, which showed reasonable toxicity against adult boll weevils. In this work, these lines were used in order to estimate the presence and relative expression of cry 10 by PCR and RT-qPCR, respectively, from young buds of 50d-plants. RT-qPCR assays were performed, by using Syber Green Rox Plus Master Mix 2X (LGC). Three endogenous cotton gene (GhACT, GhUBQ14 and GhPP2A) were used as a standard control. At first, 95°C/15 min and 40 cycles of 95°C/ 20 sec, 60°C/ 20 sec and 72°C/ 20 sec. Then, a curve of denaturation (melting curve) was performed after conclusion of amplification, at 95°C/15 sec, 60°C/15 sec, rising 2°/min until reaching 95°C. All reactions were carried out with experimental and biological replications. The threshold cycle (Ct) and PCR efficiency was estimated by Real-time PCR Miner program. The analyses were performed by using qBASEPlus program. Graphics, Cqs and Melt curve were automatically generated, based on the normalization method with a reference gene, ??Cq. Varied levels of expression were found in GM lines, too low in 8H-269 and 8H-357, mid in 8H-282 and 8H-346 and high in 8H- 336 (14x). These data agreed with previous results obtained by ELISA assays. Eleven lines derivate from 8H-336 (T1) were analyzed by PCR assays with genomic DNA, using 2 primer combinations. More than 50% showed amplicons confirming the presence of gene in selected lines. Taking in account that a reasonable level of resistance should overcoming 2 ug of protein/g tissue, we suggested that 8H-366 is the best genotype for control the cotton boll weevil. This material will be further advanced for entomological assays with larvae and adults of boll weevil. 650 $aCotton 650 $aAlgodão 650 $aAnthonomus Grandis 650 $aBicudo 653 $aBoll weevil 700 1 $aSILVA, C. R. C. da 700 1 $aSOARES, T. da C 700 1 $aCAVALCANTI, J. J. V. 700 1 $aLIMA, L. M. de 700 1 $aMARTINS, E. S. 700 1 $aPONTES, R. G. M. S. de
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Registro original: |
Embrapa Algodão (CNPA) |
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Registro Completo
Biblioteca(s): |
Embrapa Pesca e Aquicultura. |
Data corrente: |
17/03/2014 |
Data da última atualização: |
17/03/2014 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
SOZO, J. S.; COSTA, A. M.; CELESTINO, S. M. C.; MADALENA, J. O. de M.; CARDOSO, E. R.; CAMPOS, G. A.; FIGUEIRA, G. M.; SILVA, J. R. da; PEREIRA, R. de C. A.; VIANA, A. M. |
Afiliação: |
J. S. SOZO, UFSC; ANA MARIA COSTA, CPAC; SONIA MARIA COSTA CELESTINO, CPAC; JOSE ORLANDO DE MELO MADALENA, CPAC; ELIANE RIBEIRO CARDOSO; GUSTAVO AZEVEDO CAMPOS, CNPASA; GLYN MARA FIGUEIRA, UNICAMP; JOSÉ RAFAEL DA SILVA; RITA DE CASSIA ALVES PEREIRA, CNPAT; ANA MARIA VIANA, UFSC. |
Título: |
Teores de polifenóis e flavonóides em amostras de P. tenuifila cultivadas em diferentes regiões brasileiras. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
In: SIMPÓSIO LATINO AMERICANO DE CIÊNCIA DOS ALIMENTOS, 10., 2013, Campinas. Ciência de alimentos: impacto na nutrição e saúde: anais... Campinas: Unicamp, 2013. |
Páginas: |
p. 1278. |
Idioma: |
Português |
Conteúdo: |
Para orientar a cadeia produtiva, o trabalho objetivou avaliar a influência das regiões de plantio de maracujá alho sobre a biossíntese desses compostos. Para tanto, foram determinados os teores de polifenóis e flavonóides, pelos métodos espectrofotométricos de frutos de P.tenuifila, processados inteiros (POP-CPAC, 2012), cultivados e colhidos em MG(outubro), TO(janeiro), SP(maio), CE(julho) e DF(março), safra 2011. |
Palavras-Chave: |
Brasil; Maracujá alho; Passiflora tenuifila; Polifenóis; Polifenol oxidase. |
Thesagro: |
Flavonóide; Maracujá. |
Thesaurus NAL: |
Brazil; Flavonoids; Passion fruits; Polyphenols. |
Categoria do assunto: |
Q Alimentos e Nutrição Humana |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/95949/1/12617.pdf
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Marc: |
LEADER 01586naa a2200373 a 4500 001 1982510 005 2014-03-17 008 2013 bl uuuu u00u1 u #d 100 1 $aSOZO, J. S. 245 $aTeores de polifenóis e flavonóides em amostras de P. tenuifila cultivadas em diferentes regiões brasileiras.$h[electronic resource] 260 $c2013 300 $ap. 1278. 520 $aPara orientar a cadeia produtiva, o trabalho objetivou avaliar a influência das regiões de plantio de maracujá alho sobre a biossíntese desses compostos. Para tanto, foram determinados os teores de polifenóis e flavonóides, pelos métodos espectrofotométricos de frutos de P.tenuifila, processados inteiros (POP-CPAC, 2012), cultivados e colhidos em MG(outubro), TO(janeiro), SP(maio), CE(julho) e DF(março), safra 2011. 650 $aBrazil 650 $aFlavonoids 650 $aPassion fruits 650 $aPolyphenols 650 $aFlavonóide 650 $aMaracujá 653 $aBrasil 653 $aMaracujá alho 653 $aPassiflora tenuifila 653 $aPolifenóis 653 $aPolifenol oxidase 700 1 $aCOSTA, A. M. 700 1 $aCELESTINO, S. M. C. 700 1 $aMADALENA, J. O. de M. 700 1 $aCARDOSO, E. R. 700 1 $aCAMPOS, G. A. 700 1 $aFIGUEIRA, G. M. 700 1 $aSILVA, J. R. da 700 1 $aPEREIRA, R. de C. A. 700 1 $aVIANA, A. M. 773 $tIn: SIMPÓSIO LATINO AMERICANO DE CIÊNCIA DOS ALIMENTOS, 10., 2013, Campinas. Ciência de alimentos: impacto na nutrição e saúde: anais... Campinas: Unicamp, 2013.
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