Data/hora: 13/06/2024 09:59:56

Biblioteca(s): | Embrapa Semiárido.
Data corrente: | 21/07/2021
Data da última atualização: | 21/07/2021
Autoria: | SILVA, C. M. de J.; DIAS, R. de C. S.; MELO, N. F. de.
Afiliação: | CARLA MARIA DE JESUS SILVA; RITA DE CASSIA SOUZA DIAS, CPATSA; NATONIEL FRANKLIN DE MELO, CPATSA.
Título: | The effect of temperature and growth regulators on callus induction in watermelon anthers.
Ano de publicação: | 2021
Fonte/Imprenta: | Brazilian Archives of Biology and Technology, v. 64, e21180505, 2021.
ISSN: | 1678-4324
DOI: | https://doi.org/10.1590/1678-4324-2021180505
Idioma: | Inglês
Conteúdo: | Callus induction is one of the pathways required for haploid plant regeneration through anther culture. Pollen viability, as well as the effect of growth regulators and cold pretreatment on anthers of two watermelon lines (Smile and Sugar Baby) to induce callus formation were herein evaluated. Pollen viability was estimated through the staining technique using 2% acetic carmine. Male flower buds were collected and disinfested to allow removal anthers. These anthers were placed on Murashige and Skoog medium, which was supplemented with 2,4-dichlorophenoxyacetic (2,4-D) at 0.0, 0.5, 1.0, 2.0 or 5.0 ?M or with 6-benzylaminopurine at 0.0, 0.5, 1.0, 1.5, or 2.0 ?M, in combination with 2.0 ?M of 2,4-dichlorophenoxyacetic. Anthers were pretreated at 4 °C, for two days and then placed in vitro. Both watermelon lines provided high pollen viability rates (from 93 to 98%). The 2.0 and 5.0 ?M concentrations of 2,4-D stimulated higher friable callus formation. The optimal concentration of 2,4-D was estimated at 3.78 ?M and 4.17 ?M, which had callus induction rates of 64% and 52%, respectively. The combination of 2.0 ?M of 2,4-D and 6-benzylaminopurine did not lead to increased anther response to callus induction. The pre-treatment applied to flower buds at 4 °C enabled callus induction and the anther response to callus induction was genotype-dependent.
Palavras-Chave: | Calogênese de anteras; Viabilidade do pólen.
Thesagro: | Citrullus Lanatus; Cucurbitaceae; Fruta Cucurbitácea; Melancia; Regulador de Crescimento.
Thesaurus Nal: | Growth regulators; Watermelons.
Categoria do assunto: | F Plantas e Produtos de Origem Vegetal
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/224590/1/The-effect-of-temperature-and-growth-regulator-2021.pdf
Registro original: | Embrapa Semiárido (CPATSA)
Exemplares: | CPATSA - (59590-1) - UPC - AP - DD -  -  -  - DIS