Data/hora: 20/06/2024 18:11:56

Biblioteca(s): | Embrapa Rondônia.
Data corrente: | 16/11/2022
Data da última atualização: | 16/11/2022
Autoria: | SILVA, C. K. C. da; SANTOS, M. R. A. dos.
Afiliação: | CAMILA KAREN CANDEIRA DA SILVA, UNIVERSIDADE FEDERAL DE RONDÔNIA; MAURICIO REGINALDO ALVES DOS SANTOS, CPAF-RO.
Título: | Optimization of somatic embryogenesis in Coffea canephora.
Ano de publicação: | 2022
Fonte/Imprenta: | International Journal of Development Research, v. 12, n. 10, p. 59738-59740, Oct. 2022.
ISSN: | 2230-9926
DOI: | https://doi.org/10.37118/ijdr.25621.10.2022
Idioma: | Inglês
Conteúdo: | Plant tissue culture allows the clonal propagation of Coffea canephora on a large scale through somatic embryogenesis. The aim of this study was to optimize the conditions for the induction of somatic embryogenesisin leaves of the Robusta variety. The physiological stage of the leaves - drain or source; the use of the surrounding area ofprimary or secondary veins; and the position of the explants - abaxial or adaxial surface in contact with the culture medium were evaluated. The explants were inoculated in a modified MS culture medium (1/2 macro, 1/4 micronutrients), supplemented with 30 g L-1 sucrose, 6 g L-1 agar, 1.0 mg L-1 2iP and 5.0 mg L-1 IBA. At 45 days of culture, the occurrence of callus induction and the dry weight of the explants were evaluated and, at 120 days, the average number of cotyledonary embryos per explant. The leaf fragments most responsive to the induction of somatic embryogenesis were taken from drain leaves, with explants containing secondary veins, placed with the adaxial face in contact with the culture medium.
Palavras-Chave: | Amazônia Ocidental; Cotyledonary embryos; Embrapa Rondônia; Embriogênese somática; Porto Velho (RO); Western Amazon.
Thesagro: | Café Robusta; Campo Experimental; Coffea Canephora; Cultura de Tecido.
Thesaurus Nal: | Demonstration farms; Somatic embryogenesis; Tissue culture.
Categoria do assunto: | F Plantas e Produtos de Origem Vegetal
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1148351/1/cpafro-18834.pdf
Registro original: | Embrapa Rondônia (CPAF-RO)
Exemplares: | CPAF-RO - (18834-1) - UPC - AP - DD -  -  -  - DIS