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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
07/07/2014 |
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Data da última atualização: |
25/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
KAYA, E.; ALVES, A. A. C.; RODRIGUES, L.; JENDEREK, M.; HERNANDEZ-ELLIS, M.; OZUDOGRU, A.; ELLIS, D. |
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Afiliação: |
E. KAYA, USDA-ARS; ALFREDO AUGUSTO CUNHA ALVES, CNPMF; L. RODRIGUES, USDA-ARS; M. JENDEREK, USDA-ARS; M. HERNANDEZ-ELLIS, USDA-ARS; A. OZUDOGRU, CNR-IVALSA; D. ELLIS, USDA-ARS. |
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Título: |
Cryopreservation of eucalyptus genetic resources. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Cryo-Letters, v. 34, n.6, p.608-618, 2013. |
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ISSN: |
0143-2044 |
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Idioma: |
Inglês |
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Conteúdo: |
The long-term preservation of forest genetic resources is a vital part of preserving our forest crops for future generations. Unfortunately, there are few genebanks dedicated to forest trees and very few methods for long-term preservation of forest genetic resources collections aside from field plantings of a limited number of seed-derived or elite clonal individuals. The use of cryopreservation for the long-term storage of elite germplasm is increasingly being used for the long-term preservation of clonal agronomic crops but for forest trees, such as Eucalyptus, the methodology for cryopreservation of diverse genetic resources collections has not been established. We report the successful cryopreservation of a germplasm collection of in vitro shoot cultures of thirteen Eucalyptus spp. lines consisting of two E. grandis x E. camaldulensis lines, seven E. urophylla x E. grandis lines, one E. grandis line, two E. grandis x E. urophylla lines, and one E. camaldulensis line. In a comparison of two cryopreservation methods, sucrose sensitivity limited the application of encapsulation-dehydration. However, with droplet-vitrification, all thirteen lines had good survival after cryopreservation in liquid nitrogen. A 30 min exposure to Plant Vitrification Solution 2 (PVS2) yielded post-liquid nitrogen survival between 38% and 85% depending on the line. One hundred shoot tips from all thirteen lines are currently in long-term storage as a germplasm collection. |
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Thesagro: |
Criopreservação; Nitrogênio; Recurso genético. |
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Thesaurus NAL: |
Cryopreservation; Genetic resources; Nitrogen. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02203naa a2200277 a 4500
001 1989967
005 2023-05-25
008 2013 bl uuuu u00u1 u #d
022 $a0143-2044
100 1 $aKAYA, E.
245 $aCryopreservation of eucalyptus genetic resources.$h[electronic resource]
260 $c2013
520 $aThe long-term preservation of forest genetic resources is a vital part of preserving our forest crops for future generations. Unfortunately, there are few genebanks dedicated to forest trees and very few methods for long-term preservation of forest genetic resources collections aside from field plantings of a limited number of seed-derived or elite clonal individuals. The use of cryopreservation for the long-term storage of elite germplasm is increasingly being used for the long-term preservation of clonal agronomic crops but for forest trees, such as Eucalyptus, the methodology for cryopreservation of diverse genetic resources collections has not been established. We report the successful cryopreservation of a germplasm collection of in vitro shoot cultures of thirteen Eucalyptus spp. lines consisting of two E. grandis x E. camaldulensis lines, seven E. urophylla x E. grandis lines, one E. grandis line, two E. grandis x E. urophylla lines, and one E. camaldulensis line. In a comparison of two cryopreservation methods, sucrose sensitivity limited the application of encapsulation-dehydration. However, with droplet-vitrification, all thirteen lines had good survival after cryopreservation in liquid nitrogen. A 30 min exposure to Plant Vitrification Solution 2 (PVS2) yielded post-liquid nitrogen survival between 38% and 85% depending on the line. One hundred shoot tips from all thirteen lines are currently in long-term storage as a germplasm collection.
650 $aCryopreservation
650 $aGenetic resources
650 $aNitrogen
650 $aCriopreservação
650 $aNitrogênio
650 $aRecurso genético
700 1 $aALVES, A. A. C.
700 1 $aRODRIGUES, L.
700 1 $aJENDEREK, M.
700 1 $aHERNANDEZ-ELLIS, M.
700 1 $aOZUDOGRU, A.
700 1 $aELLIS, D.
773 $tCryo-Letters$gv. 34, n.6, p.608-618, 2013.
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