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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
29/11/2012 |
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Data da última atualização: |
18/05/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LIU, J.; LUO, X.; SHAFF, J.; LIANG, C.; JIA, X.; LI, Z.; MAGALHAES, J.; KOCHIAN, L. V. |
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Afiliação: |
Jiping Liu, Cornell University, Ithaca, NY.; Xiaoying Luo, Southwest University, CHINA.; Jon Shaff, Cornell University, USA; Cuiyue Liang, South China Agriculture University, China.; Xiaomin Jia, Cornell University, USA.; Ziyan Li, Northwest Sci-Tech University of Agriculture and Forestry, China; JURANDIR VIEIRA DE MAGALHAES, CNPMS; Leon V. Kochian, Cornell University, USA. |
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Título: |
A promoter-swap strategy between the AtALMT and AtMATE genes increased Arabidopsis aluminum resistance and improved carbon-use efficiency for aluminum resistance. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
The Plant Journal, Oxford, v. 71, p. 327-337, 2012. |
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DOI: |
10.1111/j.1365-313X.2012.04994.x |
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Idioma: |
Inglês |
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Conteúdo: |
The primary mechanism of Arabidopsis aluminum (Al) resistance is based on root Al exclusion, resulting from Al-activated root exudation of the Al3+-chelating organic acids, malate and citrate. Root malate exudation is the major contributor to Arabidopsis Al resistance, and is conferred by expression of AtALMT1, which encodes the root malate transporter. Root citrate exudation plays a smaller but still significant role in Arabidopsis Al resistance, and is conferred by expression of AtMATE, which encodes the root citrate transporter. In this study, we demonstrate that levels of Al-activated root organic acid exudation are closely correlated with expression of the organic acid transporter genes AtALMT1 and AtMATE. We also found that the AtALMT1 promoter confers a significantly higher level of gene expression than the AtMATE promoter. Analysis of AtALMT1 and AtMATE tissue- and cell-specific expression based on stable expression of promoter–reporter gene constructs showed that the two genes are expressed incomplementary root regions: AtALMT1 is expressed in the root apices, while AtMATE is expressed in the mature portions of the roots. As citrate is a much more effective chelator of Al3+ than malate, we used a promoter-swap strategy to test whether root tip-localized expression of the AtMATE coding region driven by the stronger AtALMT1 promoter (AtALMT1P::AtMATE) resulted in increased Arabidopsis Al resistance. Our results indicate that expression of AtALMT1P::AtMATE not only significantly increased Al resistance of the transgenic plants, but also enhanced carbon-use efficiency for Al resistance. MenosThe primary mechanism of Arabidopsis aluminum (Al) resistance is based on root Al exclusion, resulting from Al-activated root exudation of the Al3+-chelating organic acids, malate and citrate. Root malate exudation is the major contributor to Arabidopsis Al resistance, and is conferred by expression of AtALMT1, which encodes the root malate transporter. Root citrate exudation plays a smaller but still significant role in Arabidopsis Al resistance, and is conferred by expression of AtMATE, which encodes the root citrate transporter. In this study, we demonstrate that levels of Al-activated root organic acid exudation are closely correlated with expression of the organic acid transporter genes AtALMT1 and AtMATE. We also found that the AtALMT1 promoter confers a significantly higher level of gene expression than the AtMATE promoter. Analysis of AtALMT1 and AtMATE tissue- and cell-specific expression based on stable expression of promoter–reporter gene constructs showed that the two genes are expressed incomplementary root regions: AtALMT1 is expressed in the root apices, while AtMATE is expressed in the mature portions of the roots. As citrate is a much more effective chelator of Al3+ than malate, we used a promoter-swap strategy to test whether root tip-localized expression of the AtMATE coding region driven by the stronger AtALMT1 promoter (AtALMT1P::AtMATE) resulted in increased Arabidopsis Al resistance. Our results indicate that expression of AtALMT1P::AtMATE not only sig... Mostrar Tudo |
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Palavras-Chave: |
Aluminum resistance; Carbon-use efficiency; Organic acid exudation; Promoter swap; Transporter protein. |
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Thesaurus Nal: |
Arabidopsis thaliana. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02528naa a2200289 a 4500
001 1940816
005 2017-05-18
008 2012 bl uuuu u00u1 u #d
024 7 $a10.1111/j.1365-313X.2012.04994.x$2DOI
100 1 $aLIU, J.
245 $aA promoter-swap strategy between the AtALMT and AtMATE genes increased Arabidopsis aluminum resistance and improved carbon-use efficiency for aluminum resistance.$h[electronic resource]
260 $c2012
520 $aThe primary mechanism of Arabidopsis aluminum (Al) resistance is based on root Al exclusion, resulting from Al-activated root exudation of the Al3+-chelating organic acids, malate and citrate. Root malate exudation is the major contributor to Arabidopsis Al resistance, and is conferred by expression of AtALMT1, which encodes the root malate transporter. Root citrate exudation plays a smaller but still significant role in Arabidopsis Al resistance, and is conferred by expression of AtMATE, which encodes the root citrate transporter. In this study, we demonstrate that levels of Al-activated root organic acid exudation are closely correlated with expression of the organic acid transporter genes AtALMT1 and AtMATE. We also found that the AtALMT1 promoter confers a significantly higher level of gene expression than the AtMATE promoter. Analysis of AtALMT1 and AtMATE tissue- and cell-specific expression based on stable expression of promoter–reporter gene constructs showed that the two genes are expressed incomplementary root regions: AtALMT1 is expressed in the root apices, while AtMATE is expressed in the mature portions of the roots. As citrate is a much more effective chelator of Al3+ than malate, we used a promoter-swap strategy to test whether root tip-localized expression of the AtMATE coding region driven by the stronger AtALMT1 promoter (AtALMT1P::AtMATE) resulted in increased Arabidopsis Al resistance. Our results indicate that expression of AtALMT1P::AtMATE not only significantly increased Al resistance of the transgenic plants, but also enhanced carbon-use efficiency for Al resistance.
650 $aArabidopsis thaliana
653 $aAluminum resistance
653 $aCarbon-use efficiency
653 $aOrganic acid exudation
653 $aPromoter swap
653 $aTransporter protein
700 1 $aLUO, X.
700 1 $aSHAFF, J.
700 1 $aLIANG, C.
700 1 $aJIA, X.
700 1 $aLI, Z.
700 1 $aMAGALHAES, J.
700 1 $aKOCHIAN, L. V.
773 $tThe Plant Journal, Oxford$gv. 71, p. 327-337, 2012.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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| Registros recuperados : 17 | |
| 3. |  | PINEROS, M. A.; SHAFF, J. E.; MANSLANK, H. S.; ALVES, V. M. C.; KOCHIAN, L. V. Aluminum resistance in maize cannot be solely explained by root organic acid exudation. A comparative physiological study Plant Physiology, Bethesda, v. 137, n. 1, p. 231-241, 2005.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 4. | | ALVES, V. M. C.; MAGALHAES, J. V. de; SHAFF, J.; SCHAFFERT, R. E.; GUIMARAES, C. T.; KOCHIAN, L. V. Exsudação de citrato e tolerânica ao alumínio em sorgo. In: CONGRESSO NACIONAL DE MILHO E SORGO, 26.; SIMPÓSIO BRASILEIRO SOBRE A LAGARTA-DO-CARTUCHO, SPODOPTERA FRUGIPERDA, 2.; SIMPÓSIO SOBRE COLLETOTRICHUM GRAMINICOLA, 1., 2006, Belo Horizonte. Inovação para sistemas integrados de produção: trabalhos apresentados. [Sete Lagoas]: ABMS, 2006. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
|  |
| 5. | | HUFNAGEL, B.; GUIMARÃES, C. T.; CRAFT, E. J.; SHAFF, J. E.; SCHAFFERT, R. E.; KOCHIAN, L. V.; MAGALHAES, J. V. Exploiting sorghum genetic diversity for enhanced aluminum tolerance: allele mining based on the AltSB locus. Scientific Reports, v. 8, p. 1-13, July 2018. Article number: 10094.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 6. | | KOCHIAN, L.; MAGALHAES, J.; LIU, J.; HOEKENGA, O.; PINEROS, M.; SCHAFFERT, R. E.; SHAFF, J.; ALVES, V. Molecular approaches to rhizosphere research: identification and characterization of aluminum tolerance genes and their use to improve acid soil tolerance. In: INTERNATIONAL CONFERENCE RHIZOSPHERE , 2., 2007, Montpellier. Book of abstracts... Montpellier: INRA, 2007.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 7. | | ALVES, V. M. C.; GUIMARAES, C. T.; SHAFF, J.; KOCHIAN, L.; PARENTONI, S. N.; SCHAFFERT, R. E.; MAGALHÃES, J. Molecular and physiological analysis of aluminum tolerance in maize recombinant inbreds. In: INTERNATIONAL PLANT NUTRITION COLLOQUIUM, 16., 2009, Sacramento, California. Proceedings... Davis: University of California, 2009.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 8. | | LIU, J.; LUO, X.; SHAFF, J.; LIANG, C.; JIA, X.; LI, Z.; MAGALHAES, J.; KOCHIAN, L. V. A promoter-swap strategy between the AtALMT and AtMATE genes increased Arabidopsis aluminum resistance and improved carbon-use efficiency for aluminum resistance. The Plant Journal, Oxford, v. 71, p. 327-337, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 9. | | CANÇADO, G. M. A.; PINEROS, M. A.; MARON, L. G.; SHAFF, J.; CAMARGO, S. R.; MENOSSI, M.; ALVES, V. M. C.; KOCHIAN, L. V. Cloning and characterization of an ALMT1 homologue gene in Maize. In: INTERNATIONAL PLANT & ANIMAL GENOMES CONFERENCE, 15., 2007, San Diego, CA. [Proceedings...]. [S. l.: s.n.], 2007.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 10. | | KOCHIAN, L. V.; MAGALHAES, J. V. de; LIU, J.; GUIMARAES, C. T.; ALVES, V. M. C.; MARON, L.; SHAFF, J.; LYI, M.; SCHAFFERT, R. E. Elucidating the molecular determinants of aluminum tolerance in sorghum and maize. In: INTERNATIONAL PLANT & ANIMAL GENOMES CONFERENCE, 15., 2007, San Diego, CA. [Proceedings...]. [S. l.: s.n.], 2007.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 11. | | LIU, J.; MAGALHAES, J. V. de; GUIMARAES, C. T.; LANA, U. G. de P.; HOEKENGA, O.; SHAFF, J.; PINEROS, M.; WANG, Y.; SCHAFFERT, R. E.; KOCHIAN, L. Molecular cloning and characterization of AltSB, a major aluminum tolerance gene in sorghum. In: INTERNATIONAL PLANT & ANIMAL GENOMES CONFERENCE, 15., 2007, San Diego, CA. [Proceedings...]. [S. l.: s.n.], 2007.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 12. | | MATONYEI, T. K.; CHEPROT, R. K.; LIU, J.; PIÑEROS, M. A.; SHAFF, J. E.; GUDU, S.; WERE, B.; MAGALHAES, J. V.; KOCHIAN, L. V. Physiological and molecular analysis of aluminum tolerance in selected Kenyan maize lines. Plant and Soil, Dordrecht, v. 377, p. 357-367, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 13. | | MARON, L. G.; PIÑEROS, M. A.; GUIMARAES, C. T.; MAGALHAES, J. V. de; PLEIMAN, J. K.; MAO, C.; SHAFF, J.; BELICUAS, S. N. J; KOCHIAN, L. V. Two functionally distinct members of the MATE (multi-drug and toxic compound extrusion) family of transporters potentially underlie two major aluminum tolerance QTLs in maize. The Plant Journal, Oxford, v. 61, n. 5, p. 728-740, 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 14. | | ALVES, V. M. C.; MAGALHAES, J. V. de; SHAFF, J. E.; PINEROS, M. A.; PURCINO, A. A. C.; PARENTONI, S. N.; PITTA, G. V. E.; PRATES, H. T.; SCHAFFERT, R. E.; KOCHIAN, L. V. Exsudação de ácidos orgânicos como mecanismo de tolerância a alumínio em milho. In: CONGRESSO BRASILEIRO DE FISIOLOGIA VEGETAL, 8., 2001, Ilhéus. Anais... Ilhéus: SBFV, 2001. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 15. | | MAGALHAES, J. V. de; KOCHIAN, L.; LIU, J.; GUIMARAES, C.; LANA, U.; ALVES, V.; WANG, Y.; SCHAFFERT, R. E.; HOEKENGA, O.; PINEROS, M.; SHAFF, J.; KLEIN, P.; CARNEIRO, N.; COELHO, C.; TRICK, H.; CANIATO, F.; KRESOVICH, S.; MITCHELL, S. Positional cloning and association analysis of a mate gene that confers aluminum tolerance in sorghum via the AltSB locus. In: INTERNATIONAL PLANT & ANIMAL GENOMES CONFERENCE, 16., 2008, San Diego, CA. [Proceedings...]. [S. l.: s.n.], 2008. p. 13.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 16. | | HUFNAGEL, B.; SOUSA, S. M. de; ASSIS, L.; GUIMARAES, C. T.; LEISER, W.; AZEVEDO, G. C.; NEGRI, B.; LARSON, B. G.; SHAFF, J. E.; PASTINA, M. M.; BARROS, B. A.; WELTZIEN, E.; RATTUNDE, H. F. W.; VIANA, J. H.; CLARK, R. T.; FALCÃO, A.; GAZAFFI, R.; GARCIA, A. A. F.; SCHAFFERT, R. E.; KOCHIAN, L. V.; MAGALHAES, J. V. Duplicate and conquer: multiple homologs of PHOSPHORUS-STARVATION TOLERANCE1 enhance phosphorus acquisition and sorghum performance on low-phosphorus soils. Plant Physiology, Bethesda, v. 166, p. 659-677, Oct. 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 17. | | MAGALHAES, J. V. de; LIU, J.; GUIMARAES, C. T.; LANA, U. G. de P.; ALVES, V. M. C.; WANG, Y-H.; SCHAFFERT, R. E.; HOEKENGA, O. A.; PINEROS, M. A.; SHAFF, J. E.; KLEIN, P. E.; CARNEIRO, N. P.; COELHO, C. M.; TRICK, H. N.; KOCHIAN, L. V. A gene in the multidrug and toxic compound extrusion (MATE) family confers aluminum tolerance in sorghum. Nature Genetics, New York, v. 39, n. 9, p. 1156-1161, 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| Registros recuperados : 17 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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