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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
05/02/2018 |
Data da última atualização: |
06/12/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SILVA, A. S.; SEYFRIED, M.; CAMPESTRINI, L. H.; BAGGIO, S. F. Z.; MINHO, A. P.; MOLENTO, M. B.; MAURER, J. B. B. |
Afiliação: |
Andressa Soldera Silva, UFPR; Melina Seyfried, UFPR; Luciano Henrique Campestrini, UFPR; Selma Faria Zawadzki Baggio, UFPR; ALESSANDRO PELEGRINE MINHO, CPPSE; Marcelo Beltrão Molento, UFPR; Juliana Bello Baron Maurer, UFPR. |
Título: |
Assessment of anthelmintic activity and bio-guided chemical analysis of Persea americana seed extracts. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Veterinary Parasitology, v. 251, p. 34-43, feb. 2018. |
DOI: |
10.1016/j.vetpar.2017.12.019 |
Idioma: |
Inglês |
Conteúdo: |
The aim of this study was to characterize the extracts and fractions of Persea americana Mill. (Avocado) seeds and to determine the composition and the in vitro anthelmintic activity against third-stage larvae (L3) of Haemonchus contortus. The fresh (F) and dried (H) avocado seeds (PA) were subjected to extraction with hot water (W-PAF, W-PAH), ethanol (E-PAF, E-PAH) or methanol 70% (v/v), and partition with solvents of increasing polarity [n-hexane (H-PAF, H-PAH), chloroform (C-PAF, C-PAH), ethyl acetate (Ea-PAF, Ea-PAH), and n-butanol (B-PAF, B-PAH)], yielding a total of 14 extracts/fractions. After considering the yield, water solubility, and the preliminary results of the larval migration test (LMT), the E-PAF, E-PAH, H-PAF, and H-PAH were selected for further experiments. E-PAH presented an efficiency concentration of 50% (EC50) of 36 μg/mL on the LMT. E-PAH showed the greatest efficiency when its EC50 was compared to the other fractions (E-PAF = 147 μg/mL; H-PAF = 801 μg/mL; H-PAH = 77 μg/mL). After that, the E-PAH was chemically characterized, considering its quantitative polyphenolic and flavonoid contents by colorimetric and chromatographic techniques. E-PAH presented 50, 38, and 24 mg/g of dry matter of total phenol, condensed tannins (CT), and flavonoid contents, respectively. Using high performance liquid chromatography (HPLC) analysis, E-PAH had shown to have epicatechin (4.7 μg/mL), rutin (2.8 μg/mL), and chlorogenic acid (1.4 μg/mL) as its main constituents besides quercetin. These isolated compounds were evaluated using the LMT in order to relate the composition to the anthelmintic activity observed for E-PAH. Quercetin (EC50 = 7.8 μg/mL) and epicatechin (EC50 = 10 μg/mL) presented a higher efficiency than rutin (EC50 = 30 μg/mL). Chlorogenic acid was also tested with the LMT but did not present a significant efficiency. According to the results, the phenolic composition of E-PAH and the EC50 values obtained for the isolated phenols, it can be suggested that, besides the CT content, the presence of epicatechin and rutin contributed to the larvicidal activity of E-PAH. In conclusion, avocado seeds may be used as a source of polyphenols with promising anthelmintic applications. MenosThe aim of this study was to characterize the extracts and fractions of Persea americana Mill. (Avocado) seeds and to determine the composition and the in vitro anthelmintic activity against third-stage larvae (L3) of Haemonchus contortus. The fresh (F) and dried (H) avocado seeds (PA) were subjected to extraction with hot water (W-PAF, W-PAH), ethanol (E-PAF, E-PAH) or methanol 70% (v/v), and partition with solvents of increasing polarity [n-hexane (H-PAF, H-PAH), chloroform (C-PAF, C-PAH), ethyl acetate (Ea-PAF, Ea-PAH), and n-butanol (B-PAF, B-PAH)], yielding a total of 14 extracts/fractions. After considering the yield, water solubility, and the preliminary results of the larval migration test (LMT), the E-PAF, E-PAH, H-PAF, and H-PAH were selected for further experiments. E-PAH presented an efficiency concentration of 50% (EC50) of 36 μg/mL on the LMT. E-PAH showed the greatest efficiency when its EC50 was compared to the other fractions (E-PAF = 147 μg/mL; H-PAF = 801 μg/mL; H-PAH = 77 μg/mL). After that, the E-PAH was chemically characterized, considering its quantitative polyphenolic and flavonoid contents by colorimetric and chromatographic techniques. E-PAH presented 50, 38, and 24 mg/g of dry matter of total phenol, condensed tannins (CT), and flavonoid contents, respectively. Using high performance liquid chromatography (HPLC) analysis, E-PAH had shown to have epicatechin (4.7... Mostrar Tudo |
Palavras-Chave: |
Avocado. |
Thesagro: |
Haemonchus Contortus. |
Thesaurus Nal: |
epicatechin. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 03149naa a2200241 a 4500 001 2087019 005 2018-12-06 008 2018 bl uuuu u00u1 u #d 024 7 $a10.1016/j.vetpar.2017.12.019$2DOI 100 1 $aSILVA, A. S. 245 $aAssessment of anthelmintic activity and bio-guided chemical analysis of Persea americana seed extracts.$h[electronic resource] 260 $c2018 520 $aThe aim of this study was to characterize the extracts and fractions of Persea americana Mill. (Avocado) seeds and to determine the composition and the in vitro anthelmintic activity against third-stage larvae (L3) of Haemonchus contortus. The fresh (F) and dried (H) avocado seeds (PA) were subjected to extraction with hot water (W-PAF, W-PAH), ethanol (E-PAF, E-PAH) or methanol 70% (v/v), and partition with solvents of increasing polarity [n-hexane (H-PAF, H-PAH), chloroform (C-PAF, C-PAH), ethyl acetate (Ea-PAF, Ea-PAH), and n-butanol (B-PAF, B-PAH)], yielding a total of 14 extracts/fractions. After considering the yield, water solubility, and the preliminary results of the larval migration test (LMT), the E-PAF, E-PAH, H-PAF, and H-PAH were selected for further experiments. E-PAH presented an efficiency concentration of 50% (EC50) of 36 μg/mL on the LMT. E-PAH showed the greatest efficiency when its EC50 was compared to the other fractions (E-PAF = 147 μg/mL; H-PAF = 801 μg/mL; H-PAH = 77 μg/mL). After that, the E-PAH was chemically characterized, considering its quantitative polyphenolic and flavonoid contents by colorimetric and chromatographic techniques. E-PAH presented 50, 38, and 24 mg/g of dry matter of total phenol, condensed tannins (CT), and flavonoid contents, respectively. Using high performance liquid chromatography (HPLC) analysis, E-PAH had shown to have epicatechin (4.7 μg/mL), rutin (2.8 μg/mL), and chlorogenic acid (1.4 μg/mL) as its main constituents besides quercetin. These isolated compounds were evaluated using the LMT in order to relate the composition to the anthelmintic activity observed for E-PAH. Quercetin (EC50 = 7.8 μg/mL) and epicatechin (EC50 = 10 μg/mL) presented a higher efficiency than rutin (EC50 = 30 μg/mL). Chlorogenic acid was also tested with the LMT but did not present a significant efficiency. According to the results, the phenolic composition of E-PAH and the EC50 values obtained for the isolated phenols, it can be suggested that, besides the CT content, the presence of epicatechin and rutin contributed to the larvicidal activity of E-PAH. In conclusion, avocado seeds may be used as a source of polyphenols with promising anthelmintic applications. 650 $aepicatechin 650 $aHaemonchus Contortus 653 $aAvocado 700 1 $aSEYFRIED, M. 700 1 $aCAMPESTRINI, L. H. 700 1 $aBAGGIO, S. F. Z. 700 1 $aMINHO, A. P. 700 1 $aMOLENTO, M. B. 700 1 $aMAURER, J. B. B. 773 $tVeterinary Parasitology$gv. 251, p. 34-43, feb. 2018.
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Embrapa Pecuária Sudeste (CPPSE) |
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Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
07/07/1999 |
Data da última atualização: |
06/06/2022 |
Tipo da produção científica: |
Comunicado Técnico/Recomendações Técnicas |
Autoria: |
CARVALHO FILHO, O. M. de. |
Afiliação: |
ORLANDO MONTEIRO DE CARVALHO FILHO, CPATSA. |
Título: |
Silagem de leucena e de gliricídia como fontes protéicas em dietas para vacas em lactação tendo como volumoso a palma forrageira semi-desidratada. |
Ano de publicação: |
1999 |
Fonte/Imprenta: |
Petrolina: Embrapa Semi-Árido, 1999. |
Páginas: |
6 p. |
Série: |
(Embrapa Semi-Árido. Comunicado Técnico, 82). |
Idioma: |
Português |
Conteúdo: |
Silagens de leucena e de gliricidia, associadas a ureia, como fontes proteicas alternativas para formulacao de dietas de custo minimo para vacas em lactacao, em situacoes emergenciais de seca prolongada. Com base nos resultados preliminares, existe a possibilidade do uso exclusivo e emergencial da palma forrageira semi-desidratada como volumosos para vacas mesticas A/Z em lactacao, propiciando medias de leite de l0kg/vaca/dia, com perda de peso, aceitavel por curto periodo, quando associada ao fornecimento de silagens de leucena ou gliricidia (estatisticamente similares entre si), enriquecidas com ureia, permitindo formulacao de dietas a baixo custo. |
Palavras-Chave: |
Bovino de leite; Brasil; Cow milk; Feed legumes; Feeding; Forage palm; Forage palms; Gado de leite; Green manure; Leucena; Opuntia spp; Palma; Pernambuco; Petrolina; Roughage; Volumoso. |
Thesagro: |
Alimentação; Alimentação Na Seca; Dieta; Dieta Isoproteica; Espécie; Forragem; Gado Leiteiro; Gliricidia; Lactação; Leguminosa Forrageira; Leucaena; Leucaena Leucocephala; Nutrição; Nutrição Animal; Palma Forrageira; Produção animal; Silagem; Uréia; Vaca; Vaca Leiteira; Valor Nutritivo; Volumosos. |
Thesaurus NAL: |
animal nutrition; Animal production; cows; dairy cattle; dairy cows; diet; drought; feeds; Gliricidia sepium; lactation; milk production; Opuntia; silage; urea. |
Categoria do assunto: |
-- L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CPATSA/8798/1/COT82.pdf
|
Marc: |
LEADER 02583nam a2200757 a 4500 001 1134141 005 2022-06-06 008 1999 bl uuuu u0uu1 u #d 100 1 $aCARVALHO FILHO, O. M. de 245 $aSilagem de leucena e de gliricídia como fontes protéicas em dietas para vacas em lactação tendo como volumoso a palma forrageira semi-desidratada. 260 $aPetrolina: Embrapa Semi-Árido$c1999 300 $a6 p. 490 $a(Embrapa Semi-Árido. Comunicado Técnico, 82). 520 $aSilagens de leucena e de gliricidia, associadas a ureia, como fontes proteicas alternativas para formulacao de dietas de custo minimo para vacas em lactacao, em situacoes emergenciais de seca prolongada. Com base nos resultados preliminares, existe a possibilidade do uso exclusivo e emergencial da palma forrageira semi-desidratada como volumosos para vacas mesticas A/Z em lactacao, propiciando medias de leite de l0kg/vaca/dia, com perda de peso, aceitavel por curto periodo, quando associada ao fornecimento de silagens de leucena ou gliricidia (estatisticamente similares entre si), enriquecidas com ureia, permitindo formulacao de dietas a baixo custo. 650 $aanimal nutrition 650 $aAnimal production 650 $acows 650 $adairy cattle 650 $adairy cows 650 $adiet 650 $adrought 650 $afeeds 650 $aGliricidia sepium 650 $alactation 650 $amilk production 650 $aOpuntia 650 $asilage 650 $aurea 650 $aAlimentação 650 $aAlimentação Na Seca 650 $aDieta 650 $aDieta Isoproteica 650 $aEspécie 650 $aForragem 650 $aGado Leiteiro 650 $aGliricidia 650 $aLactação 650 $aLeguminosa Forrageira 650 $aLeucaena 650 $aLeucaena Leucocephala 650 $aNutrição 650 $aNutrição Animal 650 $aPalma Forrageira 650 $aProdução animal 650 $aSilagem 650 $aUréia 650 $aVaca 650 $aVaca Leiteira 650 $aValor Nutritivo 650 $aVolumosos 653 $aBovino de leite 653 $aBrasil 653 $aCow milk 653 $aFeed legumes 653 $aFeeding 653 $aForage palm 653 $aForage palms 653 $aGado de leite 653 $aGreen manure 653 $aLeucena 653 $aOpuntia spp 653 $aPalma 653 $aPernambuco 653 $aPetrolina 653 $aRoughage 653 $aVolumoso
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Embrapa Semiárido (CPATSA) |
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