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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
01/04/2022 |
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Data da última atualização: |
01/04/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PILLAI, V. V.; KEI, T. G.; GURUNG, S.; DAS, M.; SIQUEIRA, L. G. B.; CHEONG, S. H.; HANSEN, P. J.; SELVARAJ, V. |
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Afiliação: |
VIJU VIJAYAN PILLAI, Cornell University; TIFFANY G. KEI, Cornell University; SHAILESH GURUNG, Cornell University; MOUBANI DAS, Cornell University; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; SOON HON CHEONG, Cornell University; PETER J. HANSEN, University of Florida; VIMAL SELVARAJ, Cornell University. |
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Título: |
RhoA/ROCK signaling antagonizes bovine trophoblast stem cell self-renewal and regulates preimplantation embryo size and differentiation. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Development, v. 149, n. 7, dev200115, 2022. |
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DOI: |
https://doi.org/10.1242/dev.200115 |
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Idioma: |
Inglês |
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Conteúdo: |
Exponential proliferation of trophoblast stem cells (TSC) is crucial in Ruminantia to maximize numerical access to caruncles, the restricted uterine sites that permit implantation. When translating systems biology of the undifferentiated bovine trophectoderm, we uncovered that inhibition of RhoA/Rock promoted self-renewing proliferation and substantially increased blastocyst size. Analysis of transcripts suppressed by Rock inhibition revealed transforming growth factor β1 (TGFβ1) as a primary upstream effector. TGFβ1 treatment induced changes consistent with differentiation in bTSCs, a response that could be replicated by induced expression of the bovine ROCK2 transgene. Rocki could partially antagonize TGFβ1 effects, and TGFβ receptor inhibition promoted proliferation identical to Rocki, indicating an all-encompassing upstream regulation. Morphological differentiation included formation of binucleate cells and infrequent multinucleate syncytia, features we also localize in the in vivo bovine placenta. Collectively, we demonstrate a central role for TGFβ1, RhoA and Rock in inducing bTSC differentiation, attenuation of which is sufficient to sustain self-renewal and proliferation linked to blastocyst size and preimplantation development. Unraveling these mechanisms augments evolutionary/comparative physiology of the trophoblast cell lineage and placental development in eutherians. |
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Palavras-Chave: |
Blastocisto; Células-tronco; Trofoblasto. |
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Thesagro: |
Bovino; Embrião Animal; Reprodução Animal. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02295naa a2200289 a 4500
001 2141687
005 2022-04-01
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1242/dev.200115$2DOI
100 1 $aPILLAI, V. V.
245 $aRhoA/ROCK signaling antagonizes bovine trophoblast stem cell self-renewal and regulates preimplantation embryo size and differentiation.$h[electronic resource]
260 $c2022
520 $aExponential proliferation of trophoblast stem cells (TSC) is crucial in Ruminantia to maximize numerical access to caruncles, the restricted uterine sites that permit implantation. When translating systems biology of the undifferentiated bovine trophectoderm, we uncovered that inhibition of RhoA/Rock promoted self-renewing proliferation and substantially increased blastocyst size. Analysis of transcripts suppressed by Rock inhibition revealed transforming growth factor β1 (TGFβ1) as a primary upstream effector. TGFβ1 treatment induced changes consistent with differentiation in bTSCs, a response that could be replicated by induced expression of the bovine ROCK2 transgene. Rocki could partially antagonize TGFβ1 effects, and TGFβ receptor inhibition promoted proliferation identical to Rocki, indicating an all-encompassing upstream regulation. Morphological differentiation included formation of binucleate cells and infrequent multinucleate syncytia, features we also localize in the in vivo bovine placenta. Collectively, we demonstrate a central role for TGFβ1, RhoA and Rock in inducing bTSC differentiation, attenuation of which is sufficient to sustain self-renewal and proliferation linked to blastocyst size and preimplantation development. Unraveling these mechanisms augments evolutionary/comparative physiology of the trophoblast cell lineage and placental development in eutherians.
650 $aBovino
650 $aEmbrião Animal
650 $aReprodução Animal
653 $aBlastocisto
653 $aCélulas-tronco
653 $aTrofoblasto
700 1 $aKEI, T. G.
700 1 $aGURUNG, S.
700 1 $aDAS, M.
700 1 $aSIQUEIRA, L. G. B.
700 1 $aCHEONG, S. H.
700 1 $aHANSEN, P. J.
700 1 $aSELVARAJ, V.
773 $tDevelopment$gv. 149, n. 7, dev200115, 2022.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Registro Completo
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Biblioteca(s): |
Embrapa Solos. |
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Data corrente: |
12/12/2019 |
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Data da última atualização: |
16/01/2020 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
WEBBER, D. C.; MARQUES, F. A.; OLIVEIRA NETO, M. B. de; BARROS, A. H. C.; SILVA, M. S. L. da. |
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Afiliação: |
DANIEL CHAVES WEBBER, CNPS; FLAVIO ADRIANO MARQUES, CNPS; MANOEL BATISTA DE OLIVEIRA NETO, CNPS; ALEXANDRE HUGO CEZAR BARROS, CNPS; MARIA SONIA LOPES DA SILVA, CNPS. |
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Título: |
Site selection for underground dams using spatial multi-criteria evaluation in the semi-arid region of the state of Alagoas, Brazil. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
In: INTERNATIONAL SYMPOSIUM ON MANAGED AQUIFER RECHARGE, 10., 2019, Madrid. Managed aquifer recharge: local solutions to the global water crisis: proceedings... Madrid: TRAGSA, 2019. p. 236-244. ISMAR 10. |
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Idioma: |
Inglês |
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Conteúdo: |
A spatial multi-criteria evaluation is proposed to identify suitable locations for underground dams (UD) in the semi-arid state of Alagoas, Brazil. This is a strategic program, which involves a national agency, Embrapa, and the State Government of Alagoas. Suitable areas are identified by combining spatial information on topography, hydrography, pedology, and geology. The topography (slope) and hydrography line vectors were extracted treating a Digital Elevation Model. The pedological data were classified using soil attributes including salinity and sodicity. The geological data were classified according their origin and structural/tectonic factors. The pluviometry data from 78 stations were classified and, subsequently, interpolated. Each map following a basic Geographic Information System analysis was divided into three categories. The "High suitability" category was given to locations that contain ideal conditions; the "Moderate" category indicates suitable regions, but may require further analysis focused on the element which indicated a low rating note, and finally, the "Low Suitability" category, indicates environmental limitations under the criteria presented here, although may still be considered if a more detailed investigation is performed. The methodology proved to be accurate for the scale of investigation as the results coincided well with the areas where the most efficient UDs are already in operation. |
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Palavras-Chave: |
Avaliação multicritério; Seleção de sites; Semiárido. |
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Thesagro: |
Barragem Subterrânea; Sistema de Informação Geográfica. |
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Thesaurus NAL: |
Dams (hydrology); Geographic information systems. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/206927/1/Site-selection-for-underground-dams-using-spatial-multi-criteria-evaluation-in-the-semi-arid-2019.pdf
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Marc: |
LEADER 02421nam a2200241 a 4500
001 2116784
005 2020-01-16
008 2019 bl uuuu u00u1 u #d
100 1 $aWEBBER, D. C.
245 $aSite selection for underground dams using spatial multi-criteria evaluation in the semi-arid region of the state of Alagoas, Brazil.$h[electronic resource]
260 $aIn: INTERNATIONAL SYMPOSIUM ON MANAGED AQUIFER RECHARGE, 10., 2019, Madrid. Managed aquifer recharge: local solutions to the global water crisis: proceedings... Madrid: TRAGSA, 2019. p. 236-244. ISMAR 10.$c2019
520 $aA spatial multi-criteria evaluation is proposed to identify suitable locations for underground dams (UD) in the semi-arid state of Alagoas, Brazil. This is a strategic program, which involves a national agency, Embrapa, and the State Government of Alagoas. Suitable areas are identified by combining spatial information on topography, hydrography, pedology, and geology. The topography (slope) and hydrography line vectors were extracted treating a Digital Elevation Model. The pedological data were classified using soil attributes including salinity and sodicity. The geological data were classified according their origin and structural/tectonic factors. The pluviometry data from 78 stations were classified and, subsequently, interpolated. Each map following a basic Geographic Information System analysis was divided into three categories. The "High suitability" category was given to locations that contain ideal conditions; the "Moderate" category indicates suitable regions, but may require further analysis focused on the element which indicated a low rating note, and finally, the "Low Suitability" category, indicates environmental limitations under the criteria presented here, although may still be considered if a more detailed investigation is performed. The methodology proved to be accurate for the scale of investigation as the results coincided well with the areas where the most efficient UDs are already in operation.
650 $aDams (hydrology)
650 $aGeographic information systems
650 $aBarragem Subterrânea
650 $aSistema de Informação Geográfica
653 $aAvaliação multicritério
653 $aSeleção de sites
653 $aSemiárido
700 1 $aMARQUES, F. A.
700 1 $aOLIVEIRA NETO, M. B. de
700 1 $aBARROS, A. H. C.
700 1 $aSILVA, M. S. L. da
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